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Comparison of genomic and EST-derived SSR markers in phylogenetic analysis of wheat

Published online by Cambridge University Press:  16 March 2011

Agata Gadaleta ,
Angelica Giancaspro ,
Silvana Zacheo ,
Domenica Nigro ,
Stefania Lucia Giove ,
Pasqualina Colasuonno  and
Antonio Blanco
Agata Gadaleta*
Affiliation:
Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola, 165/A, 70126 Bari, Italy
Angelica Giancaspro
Affiliation:
Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola, 165/A, 70126 Bari, Italy
Silvana Zacheo
Affiliation:
Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola, 165/A, 70126 Bari, Italy
Domenica Nigro
Affiliation:
Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola, 165/A, 70126 Bari, Italy
Stefania Lucia Giove
Affiliation:
Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola, 165/A, 70126 Bari, Italy
Pasqualina Colasuonno
Affiliation:
Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola, 165/A, 70126 Bari, Italy
Antonio Blanco
Affiliation:
Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Via Amendola, 165/A, 70126 Bari, Italy
*
*Corresponding author. E-mail: agata.gadaleta@agr.uniba.it
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Abstract

Microsatellite markers (simple sequence repeats, SSRs) are used for a wide range of crop genetic and breeding applications, including genetic diversity assessment, phylogenetic analysis, genotypic profiling and marker-assisted selection. Genomic SSR (gSSR) have attracted more attention because of abundance in plant genome, reproducibility, high level of polymorphism and codominant inheritance. Recently, the availability of data for expressed sequence tags (EST), has given more emphasis to EST-derived SSRs, which belong to the transcribed regions of DNA, and are expected to be more conserved and have a higher transferability rate across species than gSSR markers. In the present study, several gSSR and EST-SSR markers were investigated for their transferability and level of DNA polymorphism in different ancestral tetraploid and diploid Triticum and Aegilops species. The same gSSR and EST-SSR markers were also evaluated for their applicability in the phylogenetic analysis of wheat. Both gSSR and EST-SSR markers showed differences for the average transferability rate and the number of alleles/locus. Phylogenetic trees based on gSSR and EST-SSR markers were in accordance with phylogenetic relations based on cytogenetic and molecular analyses.

Keywords

expressed sequence tags-simple sequence repeatsgenomic simple sequence repeatsphylogenetic analysistransferabilitywheat
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 243 - 246
Copyright
Copyright © NIAB 2011

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References

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