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Genetic combining, heterosis analysis for horticultural traits in tomato (Solanum lycopersicum L.) using ToLCV-resistant lines and molecular validation of Ty genes

Published online by Cambridge University Press:  07 January 2022

Rahul Chandel Open the ORCID record for Rahul Chandel [Opens in a new window] ,
A. T. Sadashiva ,
Kundapura V. Ravishankar Open the ORCID record for Kundapura V. Ravishankar [Opens in a new window] ,
Anjan Das Open the ORCID record for Anjan Das [Opens in a new window] ,
Bichhinna Maitri Rout Open the ORCID record for Bichhinna Maitri Rout [Opens in a new window]  and
Saurabh Singh Open the ORCID record for Saurabh Singh [Opens in a new window]
Rahul Chandel*
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
A. T. Sadashiva
Affiliation:
Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru 560089, India
Kundapura V. Ravishankar
Affiliation:
Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Bengaluru 560089, India
Anjan Das
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
Bichhinna Maitri Rout
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
Saurabh Singh
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
*
Author for correspondence: Rahul Chandel, E-mail: rahulchandel02@gmail.com
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Abstract

Tomato is a pivotal vegetable crop worldwide concerning human nutrition, economy and in service to biotechnology. Tomato leaf curl virus (ToLCV) is a begomovirus transmitted through the whitefly (Bemisia tabaci) and is responsible for severe losses in tomato production. In this context, the current investigation was carried out to determine heterotic combiners with high yield, resistance to ToLCV and mode of gene action for economically important traits. For this, 11 prescreened inbred lines were crossed in a half diallel fashion to generate 55 F1 hybrids. The 55 crosses with 11 parents and commercial checks were evaluated for different horticultural traits and resistance to ToLCV. The molecular validation with SCAR markers TG0302 and SCAR1 confirmed the presence of ToLCV-resistant genes in parents and their crosses. The hybrid IIHR-2902 × IIHR-2852 showed the presence of both Ty-2 and Ty-3 alleles in the homozygous state. The estimation of σSCA2 and σGCA2 was significant. It also indicated that the genetic control of target traits was under additive and non-additive gene effects. The values of σA/D2 along with σGCA2/σSCA2 found to be less than unity indicates the preponderance of non-additive gene action in the expression of the studied traits except for percent disease incidence. The parental line IIHR-2919 was the best combiner for fruit and yield traits. The cross combinations IIHR-2913 × IIHR-2898 exhibited significantly higher economic heterosis for yield along with the presence of Ty-2 and Ty-3 genes. The study paves the way for breeding high yielding and ToLCV-resistant hybrids in tomato.

Keywords

Heterosisleaf curl virusmolecular validationtomatoTy genesyield traits
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 6 , December 2021 , pp. 512 - 521
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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References

Ahmad, S, Quamruzzaman, AKM and Nazimuddin, M (2009) Combining ability estimates of tomato (Solanum lycopersicum) in late summer. SAARC Journal of Agriculture 7, 43–56.Google Scholar
Allard, RW (1960) Principles of Plant Breeding. New York: John Wiley & Sons.Google Scholar
Bai, Y and Lindhout, P (2007) Domestication and breeding of tomatoes: what have we gained and what can we gain in the future? Annals of Botany 100, 1085–1094.CrossRefGoogle ScholarPubMed
Baker, RJ (1978) Issues in diallel analysis. Crop Science 18, 533–536.CrossRefGoogle Scholar
Bansal, P, Banga, S and Banga, SS (2012) Heterosis as investigated in terms of polyploidy and genetic diversity using designed Brassica juncea amphiploid and its progenitor diploid species. PLoS One 7, e29607.CrossRefGoogle ScholarPubMed
Barbieri, M, Acciarri, N, Sabatini, E, Sardo, L, Accotto, GP and Pecchioni, N (2010) Introgression of resistance to two Mediterranean virus species causing tomato yellow leaf curl into a valuable traditional tomato variety. Journal of Plant Pathology 92, 485–493.Google Scholar
Bhatt, RP, Biswas, VR and Kumar, N (2001) Heterosis, combining ability and genetics for vitamin C, total soluble solids and yield in tomato (Lycopersicon esculentum) at 1700 m altitude. The Journal of Agricultural Science 137, 71–75.CrossRefGoogle Scholar
Chandel, R, Sadashiva, AT and Singh, AT (2019) Evaluation of tomato parental lines for leaf curl disease resistance and its validation through molecular markers. Indian Journal of Horticulture 76, 678–682.CrossRefGoogle Scholar
Chattopadhyay, A, Dutta, S, Dutta, P and Hazra, P (2011) Studies on heterobeltiosis, combining ability and gene action in tomato (Solanum lycopersicum). International Journal of Plant Breeding 5, 88–93.Google Scholar
Das, A, Yadav, RK, Choudhary, H, Singh, S, Khade, YP and Chandel, R (2020) Determining genetic combining ability, heterotic potential and gene action for yield contributing traits and yellow vein mosaic virus (YVMV) resistance in Okra. Plant Genetic Resources: Characterization and Utilization 1, 1–4.Google Scholar
Dhadde, SA, Patil, RV, Dharmatti, PR and Bhat, R (2009) Pooling favourable genes and enhanced heterosis through three way crosses involving potential sour tomato (Solanum lycopersicum) hybrids. Karnataka Journal of Agricultural Science 22, 1062–1068.Google Scholar
Dhatt, AS and Singh, S (2008) Genetics and potential of tomato ripening mutants under north-western plains of India. Vegetable Science 35, 14–20.Google Scholar
Dong, P, Han, K, Siddique, MI, Kwon, JK, Zhao, M and Wang, F (2016) Gene-based markers for the tomato yellow leaf curl virus resistance gene Ty-3. Plant Breeding and Biotechnology 4, 79–86.Google Scholar
Đorđević, R, Zečević, B, Zdravković, J, Živanović, T and Todorović, G (2010) Inheritance of yield components in tomato. Genetika 42, 575–583.Google Scholar
Fan, S, Brzeska, J, Keyzer, M and Halsema, A (2013) From Subsistence to Profit: Transforming Smallholder Farms. NW, Washington, DC, USA: International Food Policy Research Institute.CrossRefGoogle Scholar
FAOSTAT (2019) Food and Agriculture Organization of the United Nations (FAO) FAOSTAT Database.Google Scholar
Gerszberg, A, Hnatuszko-Konka, K, Kowalczyk, T and Kononowicz, AK (2014) Tomato (Solanum lycopersicum L.) in the service of biotechnology. Plant Cell, Tissue and Organ Culture 120, 881–902.CrossRefGoogle Scholar
Hannan, MM, Biswas, MK, Ahmed, MB, Hossain, M and Islam, R (2007 a) Combining ability analysis of yield and yield components in tomato (Lycopersicum esculentum Mill.). Turkish Journal of Botany 6, 559–563.Google Scholar
Hanson, P, Lu, SF, Wang, JF, Chen, W, Kenyon, L and Tan, CW (2016) Conventional and molecular marker-assisted selection and pyramiding of genes for multiple disease resistance in tomato. Scientia Horticulturae 201, 346–354.CrossRefGoogle Scholar
Hayes, HK, Immer, FR and Smith, DC (1965) Methods of Plant Breeding. New Delhi, India: Biotech Books, p. 552.Google Scholar
Horowitz, AR, Kontsedalov, S, Khasdan, V and Ishaaya, I (2005) Biotypes B and Q of Bemisia tabaci and their relevance to neonicoti-noid and pyriproxyfen resistance. Archives of Insect Biochemistry and Physiology 58, 216–225.CrossRefGoogle ScholarPubMed
Kaushal, A, Sadashiva, AT, Krishna, RM, Srinivasa, RE, Singh, TH, Sriram, S, Dhananjay, MV, Venugopalan, R and Ravishankar, KV (2020) Assessment of the effectiveness of Ty genes in tomato against tomato leaf curl Bangalore virus. Plant Pathology 69, 1777–1786.CrossRefGoogle Scholar
Kaushik, P and Dhaliwal, MS (2018) Diallel analysis for morphological and biochemical traits in tomato cultivated under the influence of tomato leaf curl virus. Agronomy 8, 153.CrossRefGoogle Scholar
Kumar, R, Srivastava, K, Singh, NP, Vasistha, NK, Singh, RK and Singh, MK (2013) Combining ability analysis for yield and quality traits in tomato (Solanum lycopersicum L.). Journal of Agricultural Sciences 5, 213–218.Google Scholar
Mohanty, BK and Mishra, RS (1999) Studies on heterosis for yield and yield attributes in pumpkin (Cucurbita moschata Duch. Ex, Poir.). Indian Journal of Horticulture 56, 171–178.Google Scholar
Muniyappa, V, Jalikop, SH, Saikia, AK, Channarayappa, SG, Ishwarabhat, A and Ramappa, HK (1991) Reaction of Lycopersicon cultivars and wild accessions to tomato leaf curl virus. Euphytica 56, 37–41.CrossRefGoogle Scholar
Munshi, AD and Verma, VK (1999) Combining ability in muskmelon (Cucumis melo). Indian Journal of Agricultural Sciences 69, 214–216.Google Scholar
Murray, MG and Thompson, WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8, 4321–4326.CrossRefGoogle ScholarPubMed
Naveen, G, Cheema, DS and Dhatt, AS (2008) Genetics of yield, quality and shelf life characteristics in tomato under normal and late planting conditions. Euphytica 159, 275–288.Google Scholar
Ohnishi, J, Yamaguchi, H and Saito, A (2016) Analysis of the mild strain of tomato yellow leaf curl virus, which overcomes Ty-2 gene-mediated resistance in tomato line H24. Archives of Virology 161, 2207–2217.CrossRefGoogle ScholarPubMed
Pandiarana, N, Chattopadhyay, A, Seth, T, Shende, VD, Dutta, S and Hazra, P (2015) Heterobeltiosis, potence ratio and genetic control of processing quality and disease severity traits in tomato. New Zealand Journal of Crop and Horticultural Science 43, 282–293.CrossRefGoogle Scholar
Prabhandakavi, P, Kumar, R, Palicherla, SR, Ramchandran, E and Pinnamaneni, R (2018) Occurrence and molecular detection of bipartite begomovirus on tomato in southern India. Archives of Phytopathology and Plant Protection 51, 951–955.CrossRefGoogle Scholar
Prasanna, HC, Kashyap, SP, Krishna, R, Sinha, DP, Reddy, S and Malathi, VG (2015) Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids. Euphytica 204, 407–418.CrossRefGoogle Scholar
Rattan, P and Chadha, S (2009) Gene action studies for yield and its contributing characters. Biological Forum-An International Journal 1, 8–10.Google Scholar
Salimath, PM and Bahl, PN (1985) Heterosis and combining ability for earliness in chickpea (Cicer arietinum L.). Indian Journal of Genetics and Plant Breeding 45, 97–100.Google Scholar
Sekhar, L, Prakash, BG, Salimath, PM, Hiremath, PC, Sridevi, O and Patil, AA (2010) Implications of heterosis and combining ability among productive single cross hybrids in tomato. Electronic Journal of Plant Breeding 1, 706–711.Google Scholar
Shankarappa, KS, Sriharsha, KT, Rangaswamy, DS, Aswathanarayana, HA, Prameela, RS, Kulkarni, V, Muniyappa, A, Mohanrao, MN and Maruthi, MN (2008) Development of tomato hybrids resistant to tomato leaf curl virus disease in South India. Euphytica 164, 531–539.CrossRefGoogle Scholar
Shende, VD, Seth, T, Mukherjee, S and Chattopadhyay, A (2012) Breeding tomato (Solanum lycopersicum L.) for higher productivity and better processing qualities. SABRAO Journal Breeding and Genetics 44, 302–321.Google Scholar
Shull, GH (1948) What is ‘heterosis’? Genetics 33, 439.CrossRefGoogle Scholar
Singh, AK and Asati, BS (2011) Combining ability and heterosis studies in tomato under bacterial wilt condition. Bangladesh Journal of Agricultural Research 36, 313–318.CrossRefGoogle Scholar
Singh, RK, Rai, N, Singh, M, Singh, SN and Srivastava, K (2014) Genetic analysis to identify good combiners for ToLCV resistance and yield components in tomato using interspecific hybridization. Journal of Genetics 93, 623–629.CrossRefGoogle ScholarPubMed
Singh, S, Dey, SS, Bhatia, R, Kumar, R, Sharma, K and Behera, TK (2019) Heterosis and combining ability in cytoplasmic male sterile and doubled haploid based Brassica oleracea progenies and prediction of heterosis using microsatellites. PLoS One 14, e0210772.CrossRefGoogle ScholarPubMed
Smith, KM (1935) A new virus disease of the tomato. Annals of Applied Biology 22, 731–741.CrossRefGoogle Scholar
Solieman, TH (2009) Diallel analysis of five tomato cultivars and estimation of some genetic parameters for growth and yield characters. Alexandria Science Exchange Journal 30, 274–288.Google Scholar
Solieman, TH, El-Gabry, MA and Abido, AI (2013) Heterosis, potence ratio and correlation of some important characters in tomato (Solanum lycopersicum L.). Scientia Horticulturae 150, 25–30.CrossRefGoogle Scholar
Vasudeva, RS and Samraj J, (1948) Leaf curl disease of tomato. Phytopathology 38, 364–369.Google Scholar
Vijeth, S, Dhaliwal, MS, Jindal, SK and Sharma, A (2018) Evaluation of tomato hybrids for resistance to leaf curl virus disease and for high-yield production. Horticulture, Environment, and Biotechnology 59, 699–709.CrossRefGoogle Scholar
Yang, X, Caro, M, Hutton, SF, Scott, JW, Guo, Y and Wang, X (2014) Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato. Molecular Breeding 34, 749–760.Google ScholarPubMed
Yordanov, M (1983) Heterosis in tomato. In: Frankel R (Ed.) Monograph Theoretical and Applied Genetics, vol. 6, pp. 189–219.Google Scholar
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