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Genetic diversity of high-molecular-weight glutenin subunit compositions in bread wheat landraces originated from Turkey

Published online by Cambridge University Press:  16 November 2016

Ridvan Temizgul ,
Mikail Akbulut  and
Domenico Lafiandra
Ridvan Temizgul*
Affiliation:
Department of Biology, Erciyes University, Faculty of Science, 38039 Kayseri, Turkey
Mikail Akbulut
Affiliation:
Department of Biology, Erciyes University, Faculty of Science, 38039 Kayseri, Turkey
Domenico Lafiandra
Affiliation:
Department of Agrobiology and Agrochemistry, University of Tuscia, Viterbo, Italy
*
*Corresponding author. E-mail: rtemizgul@erciyes.edu.tr
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Abstract

Focusing on 116 bread wheat landraces, this study investigated high molecular weight glutenin allele polymorphism, gene diversity, genetic variation and linkage disequilibrium (LD) in Glu-1 loci. To identify gluten alleles, sodium dodesyl sulphate-polyacrylamide, gel electrophoresis was used and for statistical analyses POPGENE software was employed. The results indicated that average genetic variation (h) was the highest in Glu-B1 (0.6421) and the lowest in Glu-A1 locus (0.4548); genetic similarity ratio (I) was the highest in Glu-B1 (1.4170); the highest average genetic diversity (Ht) was observed in Glu-B1 (0.6575) and the lowest diversity was observed in Glu-A1 (0.4558). It was also observed that genetic diversity in Glu-1 locus was largely due to intra-population variations. Inter-population gene flow was also calculated as 4.0051. Marmara and Southeastern Anatolia regions, the results further indicated, had the highest (2.8691) and lowest (0.1694) heterozygosity. Genetic erosion risk for Turkish bread wheat landraces was also seen to be high. Considering the mutual analyses of subunits of nationwide wheat landraces, it is possible to speculate about a limited migration between the landraces. LD of the landraces was largely because of this limited migration and/or epistatic natural selection. Since Turkey is known as the gene centre for major cereals including wheat, barley, rye and oat, where they diversified and spread throughout the world, studying the gluten allele diversity of Turkish bread wheat landraces is important. In addition, this study has revealed the applicability of LD, and neutrality tests to gluten protein diversity for the first time.

Keywords

allele frequencygenetic diversitygenetic similarityHMW glutenlinkage disequilibrium
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 1 , February 2018 , pp. 28 - 38
Copyright
Copyright © NIAB 2016 

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References

Autran, JC and Feillet, P (1985) Genetic and Technological basis of Protein Quality for Durum Wheat in Pasta. Protein Evaluation in Cereals and Legumes, Thessaloniki 2324 October 1985, Commission of the European Communities, Report EUR 10404 EN, pp. 59–71.Google Scholar
Branlard, G, Autran, JC and Monneveux, P (1989) High molecular weight glutenin subunits in durum wheat (Triticum durum). Theoretical and Applied Genetics 78: 353–358.Google Scholar
Brown, AHD, Feldman, MW and Nevo, E (1980) Multilocus structure of natural populations of Hordeum spontaneum. Genetics 96: 523–536.CrossRefGoogle ScholarPubMed
Brown, AHD and Feldman, MW (1981) Population structure of multilocus associations. Proceedings of the National Academy of Sciences 78: 5913–5916.Google Scholar
Caballero, L, Martin, MA and Alvarez, JB (2009) Genetic diversity for seed storage proteins in Lebanon and Turkey populations of wild diploid wheat (Triticum Urartu Thum. Ex Gandil.). Genetic Resources and Crop Evolution 56: 1117–1124.Google Scholar
Carrillo, JM, Vazquez, JF and Orellana, J (1990) Relationship between gluten strength and glutenin proteins in durum wheat cultivars. Plant Breeding 104: 325–333.CrossRefGoogle Scholar
Cong, H, Takata, K, Zong, YF, Ikeda, TM, Yanaka, M, Nagamine, T and Fujimaki, H (2005) Novel highmolecularweight glutenin subunits at the Glu-D1 locus in wheat landraces from the Xinjiang District of China and relationship with winter habit. Breeding Sciences 55: 459–463.Google Scholar
Cooke, RJ (1995) Allelic variability at the Glu-1 loci in wheat varieties. Plant Varieties and Seeds 8: 97–106.Google Scholar
Dubcovsky, J and Dvorak, J (2007) Genome plasticity a key factor in the success of polyploid wheat under domestication. Science 316: 1862–1866.Google Scholar
Ellstrand, NC, Prentice, HC and Hancock, JF (1999) Gene flow and introgression from domesticated plants into their wild relatives. Annual Review of Ecology and Systematics 30: 539–563.Google Scholar
Ewens, WJ (1972) The sampling theory of selectively neutral alleles. Theoretical Population Biology 3: 87–112.Google Scholar
Feldman, M (2001) Origin of cultivated wheat. In: Bonjean, AP and Angus, WJ (eds) The World Wheat Book: a History of Wheat Breeding. Paris, France: Lavoisier Publishing, pp. 3–56.Google Scholar
Gao, X, Appelbee, MJ, Mekuria, GT, Chalmers, KJ and Mather, DE (2012) A second over expression allele at the Glu-B1 high molecular weight glutenin locus of wheat: sequence characterisation and functional effects. Theoretical and Applied Genetics 124: 333–343.Google Scholar
Gianibelli, MC, Echaide, M, Larroque, OR, Carrillo, JM and Dubcovsky, J (2002) Biochemical and molecular characterization of Glu-1 loci in Argentinean wheat cultivars. Euphytica 128: 61–73.CrossRefGoogle Scholar
Gregova, E, Hermuth, J, Kraic, J and Dotlacil, L (1999) Protein heterogeneity in European wheat landraces and obsolete cultivars. Additional information. Genetic Resources and Crop Evolution 46: 521–528.CrossRefGoogle Scholar
Gregova, E, Hermuth, J, Kraic, J and Dotlacil, L (2006) Protein heterogeneity in European wheat landraces and obsolete cultivars. Additional information II. Genetic Resources and Crop Evolution 53: 867–871.Google Scholar
Gupta, RB, Bekes, F and Wrigley, CW (1991) Prediction of physical dough properties from glutenin subunit composition in bread wheats: correlation study. Cereal Chemistry 68: 328–333.Google Scholar
Heun, M, SchaferPregl, R, Klawan, D, Castagna, R, Accerbi, M, Borghi, B and Salamini, F (1997) Site of Einkorn wheat domestication identified by DNA fingerprinting. Science 278: 1312–1314.Google Scholar
Kaplan, M, Akar, T, Kamalak, A and Bulut, S (2014) Use of diploid and tetraploid hulled wheat genotypes for animal feeding. Turkish Journal of Agriculture and Forestry 38: 838–846.Google Scholar
Lagudah, ES, Flood, RG and Halloran, GM (1987) Variation in high molecular weight glutenin subunits in landraces of hexaploid wheat from Afghanistan. Euphytica 36: 3–9.Google Scholar
Lerner, SE, Kolman, MA and Rogers, WJ (2009) Quality and endosperm storage protein variation in Argentinean grown bread wheat. I. Allelic diversity and discrimination between cultivars. Journal of Cereal Science 49: 337–345.Google Scholar
Lewontin, RC (1972) Testing the theory of natural selection. Nature 236: 181–182.CrossRefGoogle Scholar
Li, F, Jiang, X, Wei, Y, Xia, G and Liu, S (2012) Characterization of a novel type of HMW subunit of glutenin from Australopyrum retrofractum. Gene 492: 65–70.Google Scholar
Liu, CY and Shepherd, KW (1991) Variation of LMW glutenin subunits in durum wheat cultivars associated with quality. In: Proceeding Conference of the Cereals International 913 September 1991, Brisbane, Australia, pp. 263–266.Google Scholar
Manifesto, MM, Schlatter, AR, Hopp, HE, Suarez, EY and Dubcovsky, J (2001) Quantitative evaluation of genetic diversity in wheat germoplasm using molecular markers. Crop Science 41: 682–690.Google Scholar
Manly, BFJ (1985) Spurious Test Results Due to Isolation By Distance. In: BFJ Manly (eds) The statistics of natural selection. London: Chapman & Hall, pp. 186–195.Google Scholar
Margiotta, B, Colaprico, G and Lafiandra, D (1987) Variation for protein components associated with quality in durum wheat lines and varieties. In: Proceeding of the 3rd International Workshop Gluten Proteins 69 May 1987, Budapest, pp. 314–330.Google Scholar
Marshall, DR and Brown, AHD (1975) Optimum sampling strategies in genetic conservation. In: Franked, OH and Hawkes, JG (eds) Crop Genetic Resources for Today and Tomorrow. Cambridge, London: Cambridge University Press, pp. 53–80.Google Scholar
McDermott, JM and McDonald, BA (1993) Gene flow in plant pathosystems. Annual Review of Phytopathology 31: 353–373.CrossRefGoogle Scholar
McIntosh, RA, Hart, GE and Gale, MD (1994) Catalogue of gene symbols for wheat (supplement). Annual Wheat Newsletter 40: 362–375.Google Scholar
Nakamura, H (2000) Allelic variation at high molecular weight glutenin subunit loci, Glu-A1, Glu-B1 and Glu-D1, in Japanese and Chinese hexaploid wheats. Euphytica 112: 187–193.Google Scholar
Nei, M (1972) Genetic distances between populations. The American Naturalist 106: 283–292.CrossRefGoogle Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences USA 70: 3321–3323.Google Scholar
Nei, M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.Google Scholar
Nei, M (1987) Molecular Evolutionary Genetics. New York: Columbia University Press, pp. 176–187.CrossRefGoogle Scholar
Ohta, T (1982) Linkage disequilibrium with the island model. Genetics 101: 139–155.CrossRefGoogle ScholarPubMed
Payne, PI (1987) Genetics of wheat storage protein and the effect of allelic variation on bread making quality. Annual Review of Plant Physiology 38: 141–153.Google Scholar
Payne, PI and Lawrence, GJ (1983) Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1, Glu-D1, which code for the high molecular weight subunits of glutenin in hexaploid wheat. Cereal Research Communication 11: 29–35.Google Scholar
Payne, PI, Holt, LM and Law, CN (1981) Structural and genetical studies of the high molecular subunits of wheat glutenin. I. Allelic variation in subunits amongst varieties of wheat. Theoretical and Applied Genetics 60: 229–236.Google Scholar
Payne, PI, Roberts, MS and Holt, LM (1986) Locations of genes controlling the D group of LMW glutenin subunits on the chromosome 1D of bread wheat. Genetic Resources 47: 175–179.Google Scholar
Porceddu, E, Ceoloni, C, Lafiandra, D, Tanzarella, OA and Mugnozza, GTS (1988) Genetic resources and plant breeding: problems and prospects. In: Proceeding 7th International Wheat Genetics Symposium, 13–19 July 1988, Cambridge, UK, pp. 7–22.Google Scholar
Sherwin, BW (2010) Entropy and information approaches to genetic diversity and its expression: genomic geography. Entropy 12: 1765–1798.Google Scholar
Smouse, PE and Ward, RH (1978) A comparison of the genetic infra-structure of the Ye'cuana and Yanomama: a likelihood analysis of genotypic variation among populations. Genetics 88: 611–631.Google Scholar
Terasawa, Y, Kawahara, T, Sasakuma, T and Sasanuma, T (2009) Evaluation of the genetic diversity of an Afghan wheat collection based on morphological variation, HMW glutenin subunit polymorphisms, and AFLP. Breeding Sciences 59: 361–371.Google Scholar
Terasawa, Y, Takara, K, Hirano, H, Kato, K, Kawahara, T, Saskuma, T and Sasanuma, T (2010) Genetic variation of high molecular weight glutenin subunit composition in Asian wheat. Genetic Resources and Crop Evolution 58: 283–289.Google Scholar
Turchetta, T, Ciaffi, M, Porceddu, E and Lafiandra, D (1995) Relationship between electrophoretic pattern of storage proteins and gluten strength in durum wheat landraces from Turkey. Plant Breeding 114: 406–412.Google Scholar
Van Hintum, TJL, Elings, A (1991) Assessment of glutenin and phenotypic diversity of Syrian durum wheat landraces in relation to their geographical origin. Euphytica 55: 209–215.Google Scholar
Vavilov, NI (1951) The origin, variation, immunity and breeding of cultivated plants. In: Selected writing of N.I. Vavilov, translated from the Russian by K. Starr Chester. Chronica Botanica 13: 13–66.Google Scholar
Weir, B (1979) Inferences about linkage disequilibrium. Biometrics 35: 235–254.Google Scholar
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