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Genetic diversity of native cultivated cacao accessions (Theobroma cacao L.) in Nicaragua

Published online by Cambridge University Press:  27 November 2012

Erwin Aragon ,
Claudia Rivera ,
Helena Korpelainen ,
Aldo Rojas ,
Paula Elomaa  and
Jari P. T. Valkonen
Erwin Aragon
Affiliation:
Centro Nicaragüense de Investigación Agropecuaria y Biotecnología (CNIAB), Instituto Nicaragüense de Tecnología Agropecuaria (INTA), Km 14.1 North Highway, Managua, Nicaragua
Claudia Rivera
Affiliation:
Centro Nicaragüense de Investigación Agropecuaria y Biotecnología (CNIAB), Instituto Nicaragüense de Tecnología Agropecuaria (INTA), Km 14.1 North Highway, Managua, Nicaragua
Helena Korpelainen*
Affiliation:
Department of Agricultural Sciences, University of Helsinki, PO Box 27, FI-00014, Finland
Aldo Rojas
Affiliation:
Centro Nicaragüense de Investigación Agropecuaria y Biotecnología (CNIAB), Instituto Nicaragüense de Tecnología Agropecuaria (INTA), Km 14.1 North Highway, Managua, Nicaragua
Paula Elomaa
Affiliation:
Department of Agricultural Sciences, University of Helsinki, PO Box 27, FI-00014, Finland
Jari P. T. Valkonen
Affiliation:
Department of Agricultural Sciences, University of Helsinki, PO Box 27, FI-00014, Finland
*
*Corresponding author. E-mail: helena.korpelainen@helsinki.fi
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Abstract

A total of 60 farmers' cacao accessions (Theobroma cacao L.) from Nicaragua were investigated using microsatellite markers to reveal their genetic composition and to identify potentially resistant genotypes against the black pod disease caused by Phytophthora palmivora. These accessions were compared with 21 breeders' accessions maintained locally, two Criollo accessions from Costa Rica and two accessions from Ecuador. The analyses showed a low level of differentiation among groups of farmers' accessions (FST = 0.06) and that six Nicaraguan accessions were genetically closely related to the two Criollo accessions used as a reference. In addition, seven distinct genotypes were found to have allelic composition that may indicate linkage to resistance alleles, thus being potential parental lines in future breeding programmes.

Keywords

farmers' and breeders' accessionsgenetic differentiationgenetic diversitymicrosatellitePhytophthora palmivoraresistance
Type
Short Communication
Information
Plant Genetic Resources , Volume 10 , Issue 3 , December 2012 , pp. 254 - 257
Copyright
Copyright © NIAB 2012

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References

Brown, JS, Phillips-Mora, W, Power, EJ, Krol, C, Cervantes-Martinez, C, Motamayor, JC and Schnell, RJ (2007) Mapping QTLs for resistance to frosty pod and black pod diseases and horticultural traits in Theobroma cacao L. Crop science 47: 1851–1858.CrossRefGoogle Scholar
Cuatrecasas, JA (1964) Cocoa and its allies: a taxonomic revision of the genus Theobroma. Contributions from the US National Herbarium 35: 32–46.Google Scholar
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15.Google Scholar
Lachenaud, P and Zhang, D (2008) Genetic diversity and population structure in wild stands of cacao trees (Theobroma cacao L.) in French Guiana. Annals of Forest Science 65: 310.Google Scholar
Lanaud, C, Risterucci, A, Pieretti, I, Falque, M, Bouet, A and Lagoda, PJL (1999) Isolation and characterization of microsatellites in Theobroma cacao L. Molecular Ecology 8: 2141–2152.Google Scholar
Lanaud, C, Risterucci, AM, Pieretti, I, N'Goran, JAK and Fargeas, D (2004) Characterisation and genetic mapping of resistance and defence gene analogs in cocoa (Theobroma cacao L.). Molecular Breeding 13: 211–227.CrossRefGoogle Scholar
Motamayor, JC, Lachenaud, P, da Silva e Mota, JW, Loor, R, Kuhn, DN, Brown, JS and Schnell, RJ (2008) Geographic and genetic population differentiation of the Amazonian chocolate tree (Theobroma cacao L). PLoS One 3: e3311.CrossRefGoogle ScholarPubMed
Pugh, T, Fouet, O, Risterucci, AM, Brottier, P, Abouladze, M, Deletrez, C, Courtois, B, Clement, D, Larmande, P, N'Goran, JAK and Lanaud, C (2004) A new cacao linkage map based on codominant markers: development and integration of 201 new microsatellite markers. Theoretical and Applied Genetics 108: 1151–1161.Google Scholar
Sereno, ML, Albuquerque, PSB, Vencovsky, R and Figueira, A (2006) Genetic diversity and natural population structure of cacao (Theobroma cacao L.) from the Brazilian Amazon evaluated by microsatellite markers. Conservation Genetics 7: 13–24.Google Scholar
Spence, JA (1961) Probable mechanism of resistance of varieties of cocoa to black pod disease caused by Phytophthora palmivora (Butl.) Butl. Nature 192: 278.CrossRefGoogle Scholar
Trognitz, B, Scheldeman, X, Hansel-Hohl, K, Kuant, A, Grebe, H and Hermann, M (2011) Genetic population structure of cacao plantings within a young production area in Nicaragua. PLoS One 6: e16056.Google Scholar
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