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AFLP-based molecular characterization of Brassica rapa and diversity in Canadian spring turnip rape cultivars

Published online by Cambridge University Press:  01 April 2008

S. I. Warwick ,
T. James  and
K. C. Falk
S. I. Warwick*
Affiliation:
Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, C.E.F., Ottawa, Ontario K1A 0C6, Canada
T. James
Affiliation:
Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, C.E.F., Ottawa, Ontario K1A 0C6, Canada
K. C. Falk
Affiliation:
Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada
*
*Corresponding author. E-mail: warwicks@agr.gc.ca
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Abstract

Information on genetic diversity and genetic relationships among taxa of Brassica rapa (n = 10, AA genome) is currently limited. Grown for oil, vegetable and fodder use in Europe and Asia, previous studies have indicated western and eastern groups corresponding to independent centres of origin. This study evaluated patterns and levels of genetic diversity in 93 accessions [includes 25 Agriculture and Agri-Food Canada (AAFC) breeding lines (BL)] of B. rapa based on 307 amplified fragment length polymorphisms (AFLP), testing subspecific separateness and the affiliation of four previously unassigned AA genome species (B. perviridis, B. purpuraria, B. ruvo and B. septiceps). AFLP data revealed three main clusters (I, II, III) corresponding to European (I), Indian (III), and a mixed Asian/European/Indian (II) purported origins of the taxa, with several subclusters observed in I and II. Mean AFLP polymorphism levels for Asian, European, Indian and AAFC-BL accessions were 79, 74, 66 and 62%, respectively. Few of the subspecies formed unique clusters and some, particularly subspecies chinensis and pekinensis, were assigned to several clusters. AFLP-based genetic distance information can be used by breeders to select diverse genotypes for cultivar development and fingerprinting of genotypes/cultivars. For example, a single AFLP primer pair was sufficient to uniquely identify all breeding lines in the AAFC B. rapa breeding programme.

Keywords

AFLPBrassica rapagenetic diversitygenetic relatednesstaxonomy
Type
Research Article
Information
Plant Genetic Resources , Volume 6 , Issue 1 , April 2008 , pp. 11 - 21
Copyright
Copyright © NIAB 2008

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References

Bailey, LH (1922) The cultivated Brassicas. Genets Herbarum 1(2): 53–108.Google Scholar
Bailey, LH (1930) The cultivated Brassicas. Genets Herbarum 2(5): 210–267.Google Scholar
Bailey, LH (1940) The cultivated Brassicas. Genets Herbarum 4(9): 319–330.Google Scholar
Burton, WA, Ripley, VL, Potts, DA and Salisbury, PA (2004) Assessment of genetic diversity in selected breeding lines and cultivars of canola quality Brassica juncea and their implications for canola breeding. Euphytica 136: 181–192.CrossRefGoogle Scholar
Charcosset, A and Moreau, L (2004) Use of molecular markers for the development of new cultivars and the evaluation of genetic diversity. Euphytica 137: 81–94.CrossRefGoogle Scholar
Das, S, Rajagopal, J, Bhatia, S, Srivastava, PS and Lakshmikumaran, M (1999) Assessment of genetic variation within Brassica campestris cultivars using amplified fragment length polymorphism and random amplification of polymorphic DNA markers. Journal of Biosciences (Bangalore) 24: 433–440.CrossRefGoogle Scholar
Denford, KE and Vaughan, JG (1977) A comparative study of certain seed iso-enzymes in the ten chromosome complex of Brassica campestris and its allies. Annals of Botany 41: 411–418.CrossRefGoogle Scholar
Edwards, K, Johnstone, C and Thompson, C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research 19: 1349.CrossRefGoogle ScholarPubMed
Gladis, TH and Hammer, K (1992) Die Gaterslebener Brassica-Kollektion – Brassica juncea, B. napus, B. nigra und B. rapa. Feddes Reportorium 103: 469–507.CrossRefGoogle Scholar
Guo, JX, Zhou, NY, Ma, RC and Cao, MQ (2002) Genetic diversity in Brassica rapa revealed by AFLP molecular markers. Journal of Agricultural Biotechnology 10: 138–143.Google Scholar
Hanelt, P (1986) Cruciferae. In: Schultze-Motel, J (ed.) Rudolf Mansfelds Verzeichnis landwirtschaftlicher und gärtnerischer Kulturpflanzen (ohne Zierpflanzen), vol. 1. Berlin: Akademie-Verlag, pp. 272–332.Google Scholar
Harberd, DJ (1976) Cytotaxonomic studies of Brassica and related genera. In: Vaughan, JG, MacLeod, AJ and Jones, BMG (eds) The Biology and Chemistry of the Cruciferae. London: Academic Press, pp. 47–68.Google Scholar
Huh, MK and Huh, HW (2001) AFLP fingerprinting of Brassica campestris L. ssp. napus var. nippo-oleifera Makino from Korea. Korean Journal of Biological Science 5: 101–106.CrossRefGoogle Scholar
Lee, SH (1982) Vegetable crops growing in China. Scientia Horticulturae 17: 201–209.CrossRefGoogle Scholar
Li, CW (1981) The origin, evolution, taxonomy, and hybridization of Chinese cabbage. In: Talakar, NS and Griggs, TD (eds) Chinese Cabbage. Proceedings of the First International Symposium. Taiwan: AVRDC, pp. 1–10.Google Scholar
Lombard, V, Baril, CP, Dubreuil, P, Blouet, F and Zhang, D, (2000) Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration. Crop Science 40: 1417–1425.CrossRefGoogle Scholar
Mantel, N (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27: 209–220.Google Scholar
McGrath, JM and Quiros, CF (1991) Intersubspecies hybrids and their progeny in Brassica campestris. Journal of the American Society for Horticultural Science 116: 349–355.CrossRefGoogle Scholar
McGrath, JM and Quiros, CF (1992) Genetic diversity at isozyme and RFLP loci in Brassica campestris as related to crop type and geographical origin. Theoretical and Applied Genetics 83: 783–790.CrossRefGoogle ScholarPubMed
McNaughton, IH (1976) Turnip and relatives. In: Simmonds, NW (ed.) Evolution of Crop Plants. London: Longman Group, pp. 45–48.Google Scholar
Negi, MS, Sabharwal, V, Bhat, SR and Lakshmikumaran, M (2004) Utility of AFLP markers for the assessment of genetic diversity within Brassica nigra germplasm. Plant Breeding 123: 13–16.CrossRefGoogle Scholar
Nishi, S (1980) Differentiation of Brassica crops in Asia and the breeding of ‘Hakuran’ a newly synthesized leafy vegetable. In: Tsunoda, S, Hinata, K and Gómez-Campo, C (eds) Brassica Crops and Wild Allies. Tokyo: Japan Scientific Societies Press, pp. 133–150.Google Scholar
Olsson, G (1954) Crosses within the campestris group of the genus Brassica. Hereditas 40: 398–418.CrossRefGoogle Scholar
Prakash, S and Hinata, K (1980) Taxonomy, cytogenetics and origin of crop Brassicas, a review. Opera Botanica 55: 1–57.Google Scholar
Ren, J, McFerson, JR, Li, R, Kresovich, S and Lamboy, W (1995) Identities and relationships among Chinese vegetable Brassicas as determined by random amplified polymorphic DNA markers. Journal of the American Society of Horticultural Science 120: 548–555.CrossRefGoogle Scholar
Rohlf, FJ (1997) NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System version 2.1. Setauket, NY: Exeter Software, p. 7.Google Scholar
Seyis, F, Snowdon, RJ, Lühs, W and Friedt, W (2003) Molecular characterization of novel resynthesized rapeseed (Brassica napus) lines and analysis of their genetic diversity in comparison with spring rapeseed cultivars. Plant Breeding 122: 473–478.CrossRefGoogle Scholar
Simonsen, V and Heneen, WK (1995) Genetic variation within and among different cultivars and landraces of Brassica campestris L. and B. oleracea L. based on isozymes. Theoretical and Applied Genetics 91: 346–352.CrossRefGoogle Scholar
Snowdon, RJ and Friedt, W (2004) Molecular markers in Brassica oilseed breeding: current status and future possibilities. Plant Breeding 123: 1–8.CrossRefGoogle Scholar
Sobotka, R, Dolanska, L, Curn, V and Ovesna, J (2004) Fluorescence-based AFLPs occur as the most suitable marker system for oilseed rape cultivar identification. Journal of Applied Genetics 45: 161–173.Google ScholarPubMed
Song, KM, Osborn, TC and Williams, PH (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). 2. Preliminary analysis of subspecies within B. rapa (syn. campestris) and B. oleracea. Theoretical and Applied Genetics 76: 593–600.CrossRefGoogle ScholarPubMed
Specht, CE and Diederichsen, A (2001) Brassica. In: Hanelt, P (ed.) Mansfeld's Encyclopedia of Agricultural and Horticultural Crops. vol. 3. Berlin: Springer-Verlag, pp. 1435–1465.Google Scholar
Srivastava, A, Gupta, V, Pental, D and Pradhan, AK (2001) AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea. Theoretical and Applied Genetics 102: 193–199.CrossRefGoogle Scholar
Ten, QM (1980) Origin, distribution, evolution of Chinese Cabbage. Chinese Agricultural Science 13: 1–8.Google Scholar
Toxopeus, H, Tamagishi, H and Oost, EH (1987) A.cultivar group classification of Brassica rapa L., update 1987. Eucarpia Cruciferae Newsletter 12: 5–6.Google Scholar
Tsen, M and Lee, SH (1942) A preliminary study of Chinese cultivated brassicas. Hortus Sinicus Bulletin 2: 1–32.Google Scholar
Vos, P, Hogers, R, Bleeker, M, Reijans, M, van de Lee, T, Hornes, M, Frijters, A, Pot, J, Peleman, J, Kuiper, M and Zabeau, M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.CrossRefGoogle ScholarPubMed
Wang, JL, Dan, B, Hu, SY, Tu, JX, Luan, YF, Meng, X, Zhou, G, Nimazhuoma, and Tan, L (2002) RAPD analysis for the genetic diversity of Brassica rapa in Tibet. Acta Genetica Sinica 29: 1021–1027.Google ScholarPubMed
Warwick, SI, Francis, A and La Fleche, J (2000) Guide to Wild Germplasm of Brassica and Allied Crops (tribe Brassiceae, Brassicaceae). 2nd edn.Ottawa, Canada: Agriculture and Agri-Food Canada Research Branch Publication, ECORC. Available athttp://www.brassica.info/info/publications/guide-wild-germplasm.php.Google Scholar
Warwick, SI, Gugel, RK, McDonald, T and Falk, KC (2006) Genetic variation of Ethiopian mustard (Brassica carinata A. Braun) germplasm in western Canada. Genetic Resources and Crop Evolution 53: 297–312.CrossRefGoogle Scholar
Yamagishi, H, Yui, S and Ashizawa, M (1985) Classification of leafy vegetables in Brassica campestris L. in Japan. Eucarpia Cruciferae Newsletter 10: 10–11.Google Scholar
Zhao, J, Wang, X, Deng, B, Lou, P, Wu, J, Sun, R, Xu, Z, Vromans, J, Koorneef, M and Bonnema, G (2005) Genetic relationships within Brassica rapa as inferred from AFLP fingerprints. Theoretical and Applied Genetics 110: 1301–1314.CrossRefGoogle ScholarPubMed
Zhou, T, Lu, L, Yang, G and Al-Shehbaz, IA (2001) Brassicaceae. In: ZY, Wu and Raven, PH (eds) Flora of China, Brassicaceae through Saxifragaceae, vol. 8. Beijing/St. Louis: Science Press/Missouri Botanical Garden, pp. 1–193.Google Scholar