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Development of an embryo germination protocol for shy-seeded grape (Vitis vinifera L.)

Published online by Cambridge University Press:  22 June 2021

Ashwini P. Benke Open the ORCID record for Ashwini P. Benke [Opens in a new window] ,
Ram Krishna ,
Roshni R. Samarth ,
Shweta S. Dhumal ,
Waquar A. Ansari ,
Poonam V. Shelke ,
Somnath S. Dukare  and
Major Singh
Ashwini P. Benke*
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Ram Krishna
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Roshni R. Samarth*
Affiliation:
ICAR-National Research Centre for Grapes, Pune, Maharashtra412307, India
Shweta S. Dhumal
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Waquar A. Ansari
Affiliation:
Department of Botany, Banaras Hindu University, Varanasi, Uttar Pradesh221005, India
Poonam V. Shelke
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Somnath S. Dukare
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Major Singh
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
*
*Corresponding authors. E-mail:ashwini.ashwini.chavan@gmail.com; sroshnir@gmail.com
*Corresponding authors. E-mail:ashwini.ashwini.chavan@gmail.com; sroshnir@gmail.com
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Abstract

Acquisition and germination of seeds are the most desired targets for the improvement of vegetatively propagated crops. In the present study, we developed a potential embryo germination protocol for the Red Globe grape cultivar having a low seed germination rate. Three grape berries at different developmental stages, viz. 50, 60 and 70 days after flowering (DAF), were selected for in-vitro embryo germination. Three growth media, namely Emershad and Ramming (ER), Nitsch and Nitsch (NN) and Murashige and Skoog (MS), and plant growth regulators (benzyl amino purine (BA), 0.5, 0.7 and 0.9 mg/l; indole butyric acid (IBA), 1.0, 1.5 and 2.0 mg/l; and gibberellic acid (GA), 0.1, 0.3 and 0.9 mg/l) were screened individually in different combinations with three amino acids, namely cysteine, glutamine and proline (2.0 μmol/l each). The maximum embryos germination percentage recorded at 70 DAF was 63.33, 47.78 and 45.56% in ER, NN and MS media, respectively, supplemented with 0.9 mg/l BA, 2.0 mg/l IBA, 0.9 mg/l GA and 2.0 μmol glutamine. Glutamine was found to have the most significant impact, and it improved the rescued embryos germination. The present study provides a potential recipe for a medium that can facilitate efficient germination of grape embryos.

Keywords

benzyl amino purineembryo rescuegrapegibberellic acidVitis vinifera
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 3 , June 2021 , pp. 252 - 260
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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