Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-21T12:57:16.590Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of an embryo germination protocol for shy-seeded grape (Vitis vinifera L.)

Published online by Cambridge University Press:  22 June 2021

Ashwini P. Benke Open the ORCID record for Ashwini P. Benke [Opens in a new window] ,
Ram Krishna ,
Roshni R. Samarth ,
Shweta S. Dhumal ,
Waquar A. Ansari ,
Poonam V. Shelke ,
Somnath S. Dukare  and
Major Singh
Ashwini P. Benke*
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Ram Krishna
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Roshni R. Samarth*
Affiliation:
ICAR-National Research Centre for Grapes, Pune, Maharashtra412307, India
Shweta S. Dhumal
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Waquar A. Ansari
Affiliation:
Department of Botany, Banaras Hindu University, Varanasi, Uttar Pradesh221005, India
Poonam V. Shelke
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Somnath S. Dukare
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
Major Singh
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Pune, Maharashtra410505, India
*
*Corresponding authors. E-mail:ashwini.ashwini.chavan@gmail.com; sroshnir@gmail.com
*Corresponding authors. E-mail:ashwini.ashwini.chavan@gmail.com; sroshnir@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Acquisition and germination of seeds are the most desired targets for the improvement of vegetatively propagated crops. In the present study, we developed a potential embryo germination protocol for the Red Globe grape cultivar having a low seed germination rate. Three grape berries at different developmental stages, viz. 50, 60 and 70 days after flowering (DAF), were selected for in-vitro embryo germination. Three growth media, namely Emershad and Ramming (ER), Nitsch and Nitsch (NN) and Murashige and Skoog (MS), and plant growth regulators (benzyl amino purine (BA), 0.5, 0.7 and 0.9 mg/l; indole butyric acid (IBA), 1.0, 1.5 and 2.0 mg/l; and gibberellic acid (GA), 0.1, 0.3 and 0.9 mg/l) were screened individually in different combinations with three amino acids, namely cysteine, glutamine and proline (2.0 μmol/l each). The maximum embryos germination percentage recorded at 70 DAF was 63.33, 47.78 and 45.56% in ER, NN and MS media, respectively, supplemented with 0.9 mg/l BA, 2.0 mg/l IBA, 0.9 mg/l GA and 2.0 μmol glutamine. Glutamine was found to have the most significant impact, and it improved the rescued embryos germination. The present study provides a potential recipe for a medium that can facilitate efficient germination of grape embryos.

Keywords

benzyl amino purineembryo rescuegrapegibberellic acidVitis vinifera
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 3 , June 2021 , pp. 252 - 260
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akkurt, M, Keskin, N, Shidfar, M, Shidfar, AC and Çakir, A (2013) Effects of some treatments prior to strati cation on germination in Kalecik Karası (Vitis vinifera L.) seeds. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 3: 9–13.Google Scholar
Ban, Y, Mitani, N, Sato, A, Kono, A and Hayashi, T (2016) Genetic dissection of quantitative trait loci for berry traits in interspecific hybrid grape (Vitis labruscana×Vitis vinifera). Euphytica 211: 295–310.CrossRefGoogle Scholar
Celik, H (2001) Effect of bottom heating, germination medium and gibberellic acid treatments on germination of Isabella (Vitis labrusca L.) grape seeds. Pakistan Journal of Biological Sciences 4: 953–957.Google Scholar
Deng, Q, Penner, MH and Zhao, Y (2011) Chemical composition of dietary fiber and polyphenols of five different varieties of wine grape pomace skins. Food Research International 44: 2712–2720.CrossRefGoogle Scholar
Doulati-Baneh, H, Mohammadi, SA, Labra, M, De Mattia, F, Bruni, I, Mezzasalma, V and Abdollahi, R (2015) Genetic characterization of some wild grape populations (Vitis vinifera subsp. sylvestris) of Zagros mountains (Iran) to identify a conservation strategy. Plant Genetic Resources 13: 27.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.Google Scholar
Emershad, RL and Ramming, DW (1984) In-ovulo embryo culture of Vitis vinifera LCV ‘Thompson seedless’. American Journal of Botany 71: 873–877.CrossRefGoogle Scholar
Emershad, RL, Ramming, DW and Serpe, MD (1989) In ovulo embryo development and plant formation from stenospermic genotypes of Vitis vinifera. American Journal of Botany 76: 397–402.CrossRefGoogle Scholar
Ergenoglu, F, Tangolar, SGKS and Gök, S (1996) The effects of some pre-treatments for promoting germination of grape seeds. Acta Horticulturae 441: 207–212.Google Scholar
Ergül, A, Perez-Rivera, G, Söylemezoglu, G, Kazan, K and Arroyo-Garcia, R (2011) Genetic diversity in Anatolian wild grapes (Vitis vinifera subsp. sylvestris) estimated by SSR markers. Plant Genetic Resources 9: 375.CrossRefGoogle Scholar
FAOSTAT (2018) http://www.fao.org/.Google Scholar
Guo, YS, Gao, XY, Zhao, YH and Guo, XW (2004) Preliminary report on the embryo rescue technique in Venus Seedless grape. Sino-overseas Grapevine Wine 3: 6–8.Google Scholar
Guo, Y, Zhao, Y, Li, K, Liu, Z, Lin, H, Guo, X and Li, C (2011) In vitro embryo rescue culture of F1 progenies from crosses between tetraploid grape and Vitis amurensis Rupr. African Journal of Agricultural Research 6: 4906–4909.Google Scholar
Ji, W, Li, ZQ, Zhou, Q, Yao, WK and Wang, YJ (2013) Breeding new seedless grape by means of in vitro embryo rescue. Genetics and Molecular Research 12: 859–869.CrossRefGoogle ScholarPubMed
Jiao, Y, Li, Z, Xu, K, Guo, Y, Zhang, C, Li, T, Jiang, Y, Liu, G and Xu, Y (2018) Study on improving plantlet development and embryo germination rates in in vitro embryo rescue of seedless grapevine. New Zealand Journal of Crop and Horticultural Science 46: 39–53.CrossRefGoogle Scholar
Ledbetter, CA and Shonnard, CB (1990) Improved seed development and germination of stenospermic grapes by plant growth regulators. Journal of Horticultural Sciences 65: 269–274.CrossRefGoogle Scholar
Li, G, Wang, G and Wang, Y (2013) Embryo rescue to obtain progeny from seedless grapes (Vitis vinifera L.)×wild Chinese Vitis species. Journal of Food Agriculture and Environment 11: 1058–1062.Google Scholar
Li, Z, Li, T, Wang, Y and Xu, Y (2015) Breeding new seedless grapes using in ovulo embryo rescue and marker-assisted selection. In Vitro Cellular and Developmental Biology Plant 51: 241–248.CrossRefGoogle Scholar
Li, S, Li, Z, Zhao, Y, Zhao, J, Luo, Q and Wang, Y (2020) New disease-resistant, seedless grapes are developed using embryo rescue and molecular markers. 3 Biotech 10: 4.CrossRefGoogle ScholarPubMed
Llobera, A and Cañellas, J (2007) Dietary fibre content and antioxidant activity of Manto Negro red grape (Vitis vinifera): pomace and stem. Food Chemistry 101: 659–666.CrossRefGoogle Scholar
Melyan, G, Sahakyan, A and Harutyunyan, A (2015) Micropropagation of grapevine (Vitis vinifera L.) seedless cultivar ‘Parvana’ through lateral bud development. Vitis 54: 253–255.Google Scholar
Morinaga, K (2001) Grape production in Japan. In Papademetrious, MK and Dent, FJ (1st eds) Grape Production in the Asia-Pacific Region. Bangkok, Thailand: FAO Regional Office for Asia and the Pacific, pp. 38–52.Google Scholar
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologiae Plantarum 15: 473497.Google Scholar
Nitsch, JP and Nitsch, C (1969) Haploid plants from pollen grains. Science (New York, N.Y.) 163: 85–87.CrossRefGoogle ScholarPubMed
Selim, HH, Ibrahim, FA, Fayek, MA, El-Deen, SS and Gamal, NM (2016) Effect of different treatments on germination of Romi red grape seeds. Vitis 20: 115.Google Scholar
Singh, Z and Brar, SS (1992) In vivo development of ovule in seedless and seeded cultivars of grapes (Vitis vinifera L.): a particular reference to in ovulo embryo culture. Vitis 31: 77–82.Google Scholar
Singh, NV, Singh, SK and Singh, AK (2011) Standardization of embryo rescue technique and bio-hardening of grape hybrids (Vitis vinifera L.) using arbuscular mycorrhizal fungi (AMF) under sub-tropical conditions. Vitis 50: 115–118.Google Scholar
Srivastava, S, Krishna, R, Sinha, RP and Singh, M (2017) TDZ-induced plant regeneration in Brassica oleracea L. var. botrytis: effect of antioxidative enzyme activity and genetic stability in regenerated plantlets. In Vitro Cellular and Developmental Biology Plant 53: 598–605.CrossRefGoogle Scholar
Tian, L and Wang, Y (2008) Seedless grape breeding for disease resistance by using embryo rescue. Vitis 47: 15.Google Scholar
Tian, L, Wang, Y, Niu, L and Tang, D (2008) Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp.: I. In vitro embryo rescue and plant development. Scientia Horticulturae 117: 136–141.CrossRefGoogle Scholar
Uzun, Hİ, Nuray, ÖZER, Akkurt, M, Cengiz, ÖZER, Aydin, S and Akturk, B (2019) Crossing of alphonse lavallee and regent grape cultivars for downy mildew resistant genotypes. 1. Seed germination and seedling growth. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 29: 72–78.Google Scholar
Verma, JP, Jaiswal, DK, Krishna, R, Prakash, S, Yadav, J and Singh, V (2018) Characterization and screening of thermophilic Bacillus strains for developing plant growth promoting consortium from hot spring of Leh and Ladakh region of India. Frontiers in Microbiology 9: 1293.CrossRefGoogle ScholarPubMed
Wang, L, Hu, X, Jiao, C, Li, Z, Fei, Z, Yan, X and Wang, X (2016) Transcriptome analyses of seed development in grape hybrids reveals a possible mechanism influencing seed size. BMC Genomics 17: 1–15.CrossRefGoogle ScholarPubMed
Yadav, M, Jain, S, Bhardwaj, A, Nagpal, R, Puniya, M, Tomar, R and Yadav, H (2009) Biological and medicinal properties of grapes and their bioactive constituents: an update. Journal of Medicinal Food 12: 473–484.CrossRefGoogle ScholarPubMed
Yang, J and Xiao, YY (2013) Grape phytochemicals and associated health benefits. Critical Reviews in Food Science and Nutrition 53: 1202–1225.CrossRefGoogle ScholarPubMed