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Genetic Diversity in the Brazilian Species of Adesmia DC (Leguminosae) as Assessed by RAPD

Published online by Cambridge University Press:  05 December 2024

Paula Menna Barreto Dias ,
Miguel Dall'Agnol  and
Maria Teresa Schifino-Wittmann
Paula Menna Barreto Dias
Affiliation:
Departamento de Plantas Forrageiras e Agrometeorologia, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Caixa Postal 15100, 91501-970 Porto Aiegre, RS, Brazil
Miguel Dall'Agnol
Affiliation:
Departamento de Plantas Forrageiras e Agrometeorologia, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Caixa Postal 15100, 91501-970 Porto Aiegre, RS, Brazil
Maria Teresa Schifino-Wittmann*
Affiliation:
Departamento de Plantas Forrageiras e Agrometeorologia, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Caixa Postal 15100, 91501-970 Porto Aiegre, RS, Brazil
*
* Corresponding author. E-mail: mtschif@ufrgs.br
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Abstract

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The 17 Brazilian species of Adesmia DC were analysed, using 20 primers, with regard to their randomly amplified polymorphic DNA (RAPD) patterns. From a total of 357 individuals analysed, the 20 primers produced 2249 fragments with molecular sizes ranging from 200 to 2700 bp, 56% of which were polymorphic. Average intra-population genetic similarity, estimated by Jaccard's coefficient, ranged from 0.35 in A. araujoi to 0.80 in A. punctata. Mean intra-specific genetic similarity varied greatly among species, ranging from 0.19 for A. tristis to 0.89 for A. arillata. Mean genetic similarity among the species, estimated by Dice's coefficient, was 0.56. RAPD markers were efficient at separating all the accessions analysed. The results obtained generally agreed with the partition of genetic variability expected according to the mode of reproduction.

Keywords

Adesmiaforage speciesgenetic variabilityRAPD
Type
Research Article
Information
Plant Genetic Resources , Volume 2 , Issue 1 , April 2004 , pp. 43 - 50
Copyright
© NIAB 2004

References

Alfenas, AC, Dusi, A, Zerbini Junior, FM, Robinson, IP, Micales, JA, Oliveira, JR, Dias, LAS, Scortichini, M, Bonde, MR, Alonso, SK, Junghans, TG and Brune, W (1998) Eletroforese de Isoenzimas e Proteínas Afins: Fundamentos e Aplicações em Plantas e Microorganismos. Viçosa: Editora UFV.Google Scholar
Bem Brahim, N, Salhi, A, Chtourou, N, Combes, D and Marrakchi, M (2002) Isozymic polymorphism and phylogeny of 10 Lathyrus species. Genetic Resources and Crop Evolution 49: 427–436.Google Scholar
Boldrini, II (1997) Campos do Rio Grande do Sul: caracterização fisionômica e problemtica ocupacional. Flora ilustrada do Rio Grande do Sul. Boletim do Instituto de Biociências 56. Porto Alegre: Editora da Universidade Federal do Rio Grande do Sul.Google Scholar
Brummer, EC, Bouton, JH and Kodiert, G (1995) Analysis of annual Medicago species using RAPD markers. Genome 38: 362–367.CrossRefGoogle ScholarPubMed
Buso, GSC, Rangel, PH and Ferreira, ME (1998) Analysis of genetic variability of South American wild rice populations (Oryza glumaepatula) with isozymes and RAPD markers. Molecular Ecology 1: 107–117.CrossRefGoogle Scholar
Buso, GSC, Lourenço, RT, Bianchetti, LB, Lins, TCL, Pozzobon, M, Amaral, ZPS and Ferreira, ME (2001) Espécies silvestres do gênero Capsicum coletadas na Mata Atlântica Brasileira e sua relação genética com espécies cultivadas de pimenta: uma primeira abordagem genética utilizando marcadores moleculares. Boletim Pesquisa Desenvolvimento 7: 1–22.Google Scholar
Campos-de-Quiroz, H and Ortega-Klose, F (2001) Genetic variability among elite red clover (Trifolium pratense L.) parents used in Chile as revealed by RAPD markers. Euphytica 122: 61–67.CrossRefGoogle Scholar
Chtourou-Ghorbel, N, Lauga, B, Ben Brahim, N, Combes, D and Marrakchi, M (2002) Genetic variation analysis in the genus Lathyrus using RAPD markers. Genetic Resources and Crop Evolution 49: 363–370.CrossRefGoogle Scholar
Coll, J and Zarza, A (1992) Leguminosas nativas promisoras: trebol polimorfo y babosita. Boletín de divulgación del INIA 22. Montevideo: INIA.Google Scholar
Crochemore, ML, Huyghe, C, Kerlan, MC, Durand, F and Julier, B (1996) Partitioning and distribution of RAPD variation in a set of populations of the Medicago sativa complex. Agronomie 16: 421–432.CrossRefGoogle Scholar
Croft, AM, Pang, ECK and Taylor, PWJ (1999) Molecular analysis of Lathyrus sativus L. (grasspea) and related Lathyrus species. Euphytica 107: 167–176.CrossRefGoogle Scholar
De Bustos, A, Casanova, C, Soler, C and Jouve, N (1998) RAPD variation in wild populations of four species of the genus Hordeum (Poaceae). Theoretical and Applied Genetics 96: 101–111.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1987) Isolation of plant DNA from fresh tissue. Focus 12: 13–15.Google Scholar
Gauer, L and Cavalli-Molina, S (2000) Genetic variation in natural populations of maté (Ilex paraguariensis A. St.-Hil., Aquifoliaceae) using RAPD markers. Heredity 84: 647–656.CrossRefGoogle ScholarPubMed
Gillies, ACM and Abbott, RJ (1998) Evaluation of random amplified polymorphic DNA for species identification and phylogenetic analysis in Stylosanthes (Fabaceae). Plant Systematics and Evolution 211: 201–216.CrossRefGoogle Scholar
Gustine, DL, Voigt, PW, Brummer, CE and Papadoulos, AY (2002) Genetic variation of RAPD markers for North American white clover collections and cultivars. Crop Science 42: 343–347.CrossRefGoogle Scholar
Harris, SA (1995) Systematics and randomly amplified polymorphic DNA in the genus Leucaena (Leguminosae, Mimosoideae). Plant Systematics and Evolution 197: 195–208.CrossRefGoogle Scholar
Kölliker, R, Stadelmann, FJ, Reidy, B and Nösberger, J (1999) Genetic variability of forage grass cultivars: a comparison of Festuca pratensis Huds., Lolium perenne L., and Dactylis glomerata L. Euphytica 106: 261–270.CrossRefGoogle Scholar
Kongkiatngam, P, Waterway, MJ, Fortin, MF and Coulman, BE (1995) Genetic variation within and between two cultivars of red clover (Trifolium pratense L.): comparisons of morphological, isozyme, and RAPD markers. Euphytica 84: 237–246.CrossRefGoogle Scholar
Mengoni, A, Gori, A and Bazzicalupo, M (2000) Use of RAPD and microsatellite (SSR) variation to assess genetic relationships among populations of tetraploid alfalfa, Medicago sativa. Plant Breeding 119: 311–317.CrossRefGoogle Scholar
Miotto, STS and Leitão Filho, HF (1993) Leguminosae-Faboideae, Gênero Adesmia DC. Flora ilustrada do Rio Grande do Sul. Boletim do Instituto de Biociências 52. Porto Alegre: Editora da Universidade Federal do Rio Grande do Sul.Google Scholar
Rodriguez, JM, Berke, T, Engle, L and Nienhuis, J (1999) Variation among and within Capsicum species revealed by RAPD markers. Theoretical and Applied Genetics 99: 147–156.CrossRefGoogle Scholar
Rohlf, J (2001) NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, Version 2.1. New York: Exeter Software.Google Scholar
Scheffer-Basso, SM, Jacques, AVA, Dall'Agnol, M, Riboldi, J and Castro, SMJ (2001) Availability and nutritive value of the wild leguminous Adesmia DC. and exotics Lotus L. Revista Brasileira de Zootecnia 30: 975–982.CrossRefGoogle Scholar
Sun, Q, Ni, Z, Liu, Z, Gao, J and Huang, T (1998) Genetic relationships and diversity among Tibetan wheat, common wheat and European spelt wheat revealed by RAPD markers. Euphytica 99: 205–211.CrossRefGoogle Scholar
Sweeney, PM and Danneberger, KT (1994) Random amplified polymorphic DNA in perennial ryegrass: a comparison of bulk samples vs. individuals. Hortscience 29: 624–626.CrossRefGoogle Scholar
Tedesco, SB, Dall'Agnol, M and Schifino-Wittmann, MT (1998) Observações sobre o modo de reprodução em Adesmia latifolia Spreng Vog. Ciência Rural 28: 141–142.CrossRefGoogle Scholar
Tedesco, SB, Dall'Agnol, M, Schifino-Wittmann, MT and Valls, JFM (2000) Mode of reproduction of Brazilian species of Adesmia (Leguminosae). Genetics and Molecular Biology 23: 475–478.CrossRefGoogle Scholar
Tedesco, SB, Schifino-Wittmann, M and Dall'Agnol, M (2002) Meiotic behaviour and pollen fertility in Brazilian species of Adesmia DC. (Leguminosae). Caryologia 55: 341–347.CrossRefGoogle Scholar
Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA and Tingey, SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.CrossRefGoogle ScholarPubMed
Yu, K and Pauls, KP (1993) Rapid estimation of genetic relatedness among heterogeneous populations of alfalfa by random amplification of bulked DNA samples. Theoretical and Applied Genetics 86: 788–794.CrossRefGoogle ScholarPubMed