Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-23T03:32:35.715Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic polymorphisms among and between blast disease resistant and susceptible finger millet, Eleusine coracana (L.) Gaertn.

Published online by Cambridge University Press:  08 March 2016

Dipnarayan Saha Open the ORCID record for Dipnarayan Saha [Opens in a new window] ,
Rajeev Singh Rana ,
Lalit Arya ,
Manjusha Verma ,
M. V. Channabyre Gowda  and
Hari D. Upadhyaya
Dipnarayan Saha*
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
Rajeev Singh Rana
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
Lalit Arya
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
Manjusha Verma
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
M. V. Channabyre Gowda
Affiliation:
All India Co-ordinated Small Millets Improvement Project, ICAR, GKVK, Bengaluru-560065, India
Hari D. Upadhyaya
Affiliation:
International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Hyderabad 502324, India
*
*Corresponding author. E-mail: dipsaha72@yahoo.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Fungal blast disease is one of the major constraints in finger millet production. Breeding for disease resistance in finger millet, needs characterization of genetic polymorphism among and between the resistant and susceptible genotypes. In total, 67 finger millet genotypes, which are resistant or susceptible to fungal blast disease, were analysed using sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers to assess genetic variations and select diverse parents. Twelve each of SRAP and SSR primers produced 95.1 and 93.1% polymorphic bands and grouped them into unweighted pair-group method with arithmetic average clusters. Two of the finger millet genotypes, IE 4709 (blast resistant) and INDAF 7 (susceptible) were distinguished as most diverse genotypes as parents. Several genotype-specific bands observed with SSR primers are potential in developing genotype-specific markers. A high genetic diversity within the resistant and susceptible genotypes, rather than between them, was revealed through Nei's gene diversity (h) index and analysis of molecular variance. The finding helps us to understand the extent of genetic polymorphism between blast disease resistant and susceptible finger millet genotypes to exploit in resistance breeding programs.

Keywords

finger milletgenetic variabilityresistant and susceptible genotypessequence related amplified polymorphism (SRAP)simple sequence repeat (SSR)
Type
Research Article
Information
Plant Genetic Resources , Volume 15 , Issue 4 , August 2017 , pp. 355 - 365
Copyright
Copyright © NIAB 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

Present address: Division of Crop Improvement, ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.

References

Arya, L, Verma, M, Gupta, VK and Seetharam, A (2013) Use of genomic and genic SSR markers for assessing genetic diversity and population structure in Indian and African finger millet (Eleusine coracana (L.) Gaertn.) germplasm. Plant Systematics and Evolution 299: 1395–1401.CrossRefGoogle Scholar
Babu, BK, Senthil, N, Gomez, SM, Biji, KR, Rajendraprasad, NS, Satheesh Kumar, S and Babu, RC (2007) Assessment of genetic diversity among finger millet (Eleusine coracana (L.) Gaertn.) accessions using molecular markers. Genetic Resources and Crop Evolution 54: 399–404.CrossRefGoogle Scholar
Babu, TK, Thakur, RP, Reddy, PN, Upadhyaya, HD, Girish, AG and Sarma, NDRK (2012) Development of a field screening technique and identification of blast resistance in finger millet core collection. Indian Journal of Plant Protection 40: 45–51.Google Scholar
Babu, TK, Thakur, RP, Upadhyaya, HD, Reddy, PN, Sharma, R, Girish, AG and Sarma, NDRK (2013) Resistance to blast (Magnaporthe grisea) in a mini-core collection of finger millet germplasm. European Journal of Plant Pathology 135: 299–311.CrossRefGoogle Scholar
Babu, BK, Dinesh, P, Agrawal, PK, Sood, S, Chandrashekara, C, Bhatt, JC and Kumar, A (2014) Comparative genomics and association mapping approaches for blast resistant genes in finger millet using SSRs. PLoS ONE 9: e99182. doi: 10.1371/journal.pone.0099182 CrossRefGoogle ScholarPubMed
Chethan, S and Malleshi, NG (2007) Finger millet polyphenols: characterization and their nutraceutical potential. American Journal of Food Technology 2: 618–629.CrossRefGoogle Scholar
Dida, MM and Devos, KM (2006) Finger millet. In: Kole, C (ed.) Cereals and Millets. NY, New York: Springer, pp. 333–343.CrossRefGoogle Scholar
Dida, MM, Srinivasachary Ramakrishnan, S, Bennetzen, JL, Gale, MD and Devos, KM (2007) The genetic map of finger millet, Eleusine coracana . Theoretical and Applied Genetics 114: 321–332.CrossRefGoogle ScholarPubMed
Dida, MM, Wanyera, N, Harrison Dunn, ML, Bennetzen, JL and Devos, KM (2008) Population structure and diversity in finger millet (Eleusine coracana) germplasm. Tropical Plant Biology 1: 131–141.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15.Google Scholar
Fakrudin, B, Shashidhar, HE, Kulkarni, RS and Hittalmani, S (2004) Genetic diversity assessment of finger millet, Eleusine coracana (Gaertn.), germplasm through RAPD analysis. PGR Newsletter 138: 50–54.Google Scholar
Gowda, J, Suvarna, , Somu, G, Bharathi, S and Mathur, PN (2007) Formation of core set in finger millet (Eleusine coracana (L.) Gaertn.) germplasm using geographical origin and morpho-agronomic characters. Indian Journal of Plant Genetic Resources 20: 38–42.Google Scholar
Lenne, JM, Takan, JP, Wanyera, N, Manyasa, EO, Mgonja, MA, Okwadi, J, Brown, AE and Sreenivasaprasad, S (2007) Finger millet blast management: a key entry point for fighting malnutrition and poverty in East Africa. Outlook on Agriculture 36: 101–108. http://dx.doi.org/10.5367/000000007781159994 CrossRefGoogle Scholar
Li, G and Quiros, CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103: 455–461. doi: 10.1007/s001220100570 CrossRefGoogle Scholar
Malambane, G, Jaisil, P, Sanitchon, J, Suriharn, B and Jothityangkoon, D (2013) Evaluation of genetic variation among finger millet (Eleusine coracana L. Gaertn) accessions using RAPD markers. SABRAO Journal of Breeding and Genetics 45: 231–239.Google Scholar
Mantel, N (1967) The detection of disease clustering and generalized regression approach. Cancer Research 27: 209–220.Google ScholarPubMed
Mantur, SG, Vishwanath, S and Anilkumar, TB (2001) Evaluation of finger millet genotypes for resistance to blast. Indian Phytopathology 54: 38–41.Google Scholar
Mbithi-Mwikya, S, Ooghe, W, Van Camp, J, Nagundi, D and Huyghebaert, A (2000) Amino acid profile after sprouting, autoclaving and lactic acid fermentation of finger millet (Eleusine coracana) and kidney beans (Phaseolus vulgaris L.). Journal of Agricultural Food Chemistry 48: 3081–3085.CrossRefGoogle ScholarPubMed
Mohammadi, SA and Prasanna, BM (2003) Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Science 43: 1235–1248.CrossRefGoogle Scholar
Nagaraja, A, Jagadish, PS, Ashok, EG and Krishne Gowda, KT (2007) Avoidance of finger millet blast by ideal sowing time and assessment of varietal performance under rainfed production situations in Karnataka. Journal of Mycopathological Research 46: 109–111.Google Scholar
Nagaraja, A, Kumar, B, Raguchander, T, Hota, AK, Patro, TSSK, Gowda, D., Savita, E and Gowda, MVC (2012) Impact of disease management practices on finger millet blast and grain yield. Indian Phytopathology 65: 356–359.Google Scholar
Nakayama, H, Nagamine, T and Hayashi, N (2005) Genetic variation of blast resistance in foxtail millet (Setaria italica (L.) P. Beauv.) and its geographic distribution. Genetic Resources and Crop Evolution 52: 863–868.CrossRefGoogle Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of National Academy of Sciences of the United States of America 70: 3321–3323.CrossRefGoogle ScholarPubMed
Nirgude, M, Babu, BK, Shambhavi, Y, Singh, UM, Upadhyaya, HD and Kumar, A (2014) Development and molecular characterization of genic molecular markers for grain protein and calcium content in finger millet (Eleusine coracana (L.) Gaertn.). Molecular Biology Reporter 41: 1189–200.CrossRefGoogle ScholarPubMed
Panwar, P, Nath, M, Yadav, VK and Kumar, A (2010) Comparative evaluation of genetic diversity using RAPD, SSR and cytochrome P450 gene based markers with respect to calcium content in finger millet (Eleusine coracana L. Gaertn.). Journal of Genetics 89: 121–133.CrossRefGoogle ScholarPubMed
Peakall, R and Smouse, PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.CrossRefGoogle Scholar
Prajapati, VP, Sabalpara, AN and Pawar, DM (2013) Assessment of yield loss due to finger millet blast caused by Pyricularia grisea (Cooke) Sacc. Trends in Biosciences 6: 876–878.Google Scholar
Prevost, A and Wilkinson, MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics 98: 107–112.CrossRefGoogle Scholar
Rajanna, MP, Rangaswamy, BR, Basavaraju, MK, Karegowda, C and Ramaswamy, GR (2000) Evaluation of finger millet genotypes for resistance to blast caused by Pyricularia grisea Sacc. Plant Disease Research 15: 199–201.Google Scholar
Rao, ANS (1990) Estimates of losses in finger millet (Eleusine coracana) due to blast disease (Pyricularia grisea). Mysore Journal of Agricultural Sciences 24: 57–60.Google Scholar
Roldan-Ruiz, I, Dendauw, J, Van Bockstaele, E, Depicker, A and De Loose, M (2000) AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding 6: 125–134.CrossRefGoogle Scholar
Rohlf, FJ (1998) NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.0 User Guide. Applied Biostatistics Inc, Setauket, New York, pp. 37.Google Scholar
Salimath, SS, de Oliveira, AC, Godwin, ID and Bennetzen, JL (1995) Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers. Genome 38: 757–763.Google ScholarPubMed
Singh, P and Raghuvanshi, RS (2012) Finger millet for food and nutritional security. African Journal of Food Sciences 64: 77–84.Google Scholar
Sneath, P and Sokal, R (1973) Numerical Taxonomy. San Francisco: WH Freeman.Google Scholar
Takan, JP, Chipili, J, Muthumeenakshi, S, Talbot, NJ, Manyasa, EO, Bandyopadhyay, R, Sere, Y, Nutsugah, SK, Talhinhas, P, Hossain, M, Brown, AE and Sreenivasaprasad, S (2012) Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction. Molecular Biotechnology 50: 145–158.CrossRefGoogle ScholarPubMed
Tomar, J, Saini, N, Goyal, BS, Tripathi, N, Shrivastava, AN, Verma, RK and Tiwari, S (2011) Assessment of genetic diversity among rhizoctonia root rot resistant soybean genotypes. Journal of Food Legumes 24: 267–272.Google Scholar
Upadhyaya, HD, Sarma, NDRK, Ravishankar, CR, Albrecht, T, Narasimhudu, Y, Singh, SK, Varshney, SK, Reddy, VG, Singh, S, Dwivedi, SL, Wanyera, N, Oduori, COA, Mgonja, MA, Kisandu, DB, Parzies, HK and Gowda, CLL (2010) Developing a mini-core collection in finger millet using multilocation data. Crop Science 50: 1924–1931.CrossRefGoogle Scholar
Upadhyaya, HD, Ramesh, S, Sharma, S, Singh, SK, Varshney, RK, Sarma, NDRK, Ravishankar, CR, Narasimhudu, Y, Reddy, VG, Sahrawat, KL, Dhanalakshmi, TN, Mgonja, MA, Parzies, HK, Gowda, CLL and Singh, S (2011) Genetic diversity for grain nutrients contents in a core collection of finger millet (Eleusine coracana (L.) Gaertn.) germplasm. Field Crops Research 121: 42–52.CrossRefGoogle Scholar
Yap, IV and Nelson, RJ (1996) Winboot: A Program for Performing Bootstrap Analysis of Binary Data to Determine the Confidence of UPGMA-Based Dendrograms. Manilla, Phillipines: IRRI.Google Scholar
Yeh, FC and Boyle, TJB (1999) POPGENE version 1.3.2: Microsoft window-based freeware for population genetic analysis. http://www.ualberta.ca/~fyeh/index.htm Google Scholar
Zeng, B, Zhang, Y, Huang, L, Jiang, X, Luo, D and Yin, G (2014) Genetic diversity of orchard grass (Dactylis glomerata L.) germplasms with resistance to rust diseases revealed by start codon targeted (SCoT) markers. Biochemical Systematics and Ecology 54: 96–102.CrossRefGoogle Scholar
Supplementary material: File

Saha supplementary material

Tables S1 and S2

Download Saha supplementary material(File)
File 27.6 KB