Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-22T10:57:19.489Z Has data issue: false hasContentIssue false
  • English
  • Français

Turnover of the Soil Organic Matter Amino Acid Fraction Investigated by 13C and 14C Signatures of Carboxyl Carbon

Published online by Cambridge University Press:  14 December 2016

Christine Hatté ,
Claude Noury ,
Louay Kheirbeik  and
Jérôme Balesdent
Christine Hatté*
Affiliation:
Laboratoire des Sciences du Climat et de l’Environnement, LSCE/IPSL, UMR8212 CEA-CNRS-UVSQ, Université Paris Saclay, 91198 Gif-sur-Yvette, France
Claude Noury
Affiliation:
Laboratoire des Sciences du Climat et de l’Environnement, LSCE/IPSL, UMR8212 CEA-CNRS-UVSQ, Université Paris Saclay, 91198 Gif-sur-Yvette, France
Louay Kheirbeik
Affiliation:
Aix Marseille Univ, CNRS, IRD, College de France, INRA, CEREGE, Aix-en-Provence, France
Jérôme Balesdent
Affiliation:
Aix Marseille Univ, CNRS, IRD, College de France, INRA, CEREGE, Aix-en-Provence, France
*
*Corresponding author. Email: Christine.Hatte@lsce.ipsl.fr.
Get access Rights & Permissions [Opens in a new window]

Abstract

Nitrogenous compounds of soil organic matter constitute a major N reservoir on Earth. Both the world food protein supply produced by agriculture and the global contamination by reactive nitrogen species rely on the dynamics of these compounds. To investigate their dynamics, we used both natural 13C labeling and accelerator mass spectrometry (AMS) 14C dating of the α-carboxyl amino carbon, which is specific of the amino acid fraction that was extracted from bulk soil organic matter by ninhydrin hydrolysis. We applied this isotopic approach to investigate the age of carboxyl carbon in a maize-cultivated Cambisol chronosequence. Based on a few measurements, we demonstrate the feasibility of this new compound-specific method of investigation of soil carbon dynamics. We show that soil organic matter amino acids can be split into two very distinct dynamic compartments: the majority having a mean age of a few years and a minority having a mean carbon age of several millennia. The latter fraction can be either strongly stabilized in soils, or can arise from microbial utilization of old carbon resources, as predicted by the priming effect theory.

Keywords

soilsgeochemistryradiocarbonstable isotopes
Type
Cosmogenic Isotopes in Studies of Soil Dynamics
Information
Radiocarbon , Volume 59 , Special Issue 2: Proceedings of the 22nd International Radiocarbon Conference (Part 1 of 2) , April 2017 , pp. 473 - 481
Copyright
© 2016 by the Arizona Board of Regents on behalf of the University of Arizona 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Selected Papers from the 2015 Radiocarbon Conference, Dakar, Senegal, 16–20 November 2015

References

REFERENCES

Apostel, C, Dippold, M, Glaser, B, Kuzyakov, Y. 2013. Biochemical pathways of amino acids in soil: assessment by position-specific labeling and 13C-PLFA analysis. Soil Biology and Biochemistry 67:31–40.Google Scholar
Balesdent, J, Mariotti, A. 1996. Measurement of soil organic matter turnover using 13C natural abudances. In: Boutton TW, Yamasaki S-I, editors. Mass Spectrometry in Soils. New York: Marcel Dekker. p 83–111.Google Scholar
Bol, R, Poirier, N, Balesdent, J, Gleixner, G. 2009. Molecular turnover time of soil organic matter in particle-size fractions of an arable soil. Rapid Communications in Mass Spectrometry 23(16):2551–2558.Google Scholar
Dignac, MF, Bahri, H, Rumpel, C, Rasse, DP, Bardoux, G, Balesdent, J, Girardin, C, Chenu, C, Mariotti, A. 2005. Carbon-13 natural abundance as a tool to study the dynamics of lignin monomers in soil: an appraisal at the Closeaux experimental field (France). Geoderma 128(1–2):3–17.CrossRefGoogle Scholar
Dippold, M, Biryukov, M, Kuzyakov, Y. 2014. Sorption affects amino acid pathways in soil: implications from position-specific labeling of alanine. Soil Biology and Biochemistry 72:180–192.Google Scholar
Fontaine, S, Barot, S, Barré, P, Bdioui, N, Mary, B, Rumpel, C. 2007. Stability of organic carbon in deep soil layers controlled by fresh carbon supply. Nature 450(7167):277–280.Google Scholar
Friedel, JK, Scheller, E. 2002. Composition of hydrolysable amino acids in soil organic matter and soil microbial biomass. Soil Biology and Biochemistry 34(3):315–325.Google Scholar
Geisseler, D, Horwath, WR. 2014. Investigating amino acid utilization by soil microorganisms using compound specific stable isotope analysis. Soil Biology and Biochemistry 74:100–105.Google Scholar
Geisseler, D, Horwath, WR, Joergensen, RG, Ludwig, B. 2010. Pathways of nitrogen utilization by soil microorganisms – a review. Soil Biology and Biochemistry 42(12):2058–2067.Google Scholar
Gleixner, G, Bol, R, Balesdent, J. 1999. Molecular insight into soil carbon turnover. Rapid Communications in Mass Spectrometry 13(13):1278–1283.3.0.CO;2-N>CrossRefGoogle ScholarPubMed
Gleixner, G, Poirier, N, Bol, R, Balesdent, J. 2002. Molecular dynamics of organic matter in a cultivated soil. Organic Geochemistry 33(3):357–366.Google Scholar
Gruber, N, Galloway, JN. 2008. An Earth-system perspective of the global nitrogen cycle. Nature 451(7176):293–296.Google Scholar
Holub, SM, Lajtha, K. 2004. The fate and retention of organic and inorganic 15N-nitrogen in an old-growth forest soil in Western Oregon. Ecosystems 7(4):368–380.CrossRefGoogle Scholar
Hua, Q, Barbetti, M, Rakowski, AZ. 2013. Atmospheric radiocarbon for the period 1950–2010. Radiocarbon 56(4):2059–2072.Google Scholar
Jull, AJT, Burr, GS, Beck, JW, Donahue, DJ, Biddulph, D, Hatheway, AL, Lange, TE, McHargue, LR. 2003. Accelerator mass spectrometry at Arizona: geochronology of the climate record and connections with the ocean. Journal of Environmental Radioactivity 69(1–2):3–19.Google Scholar
Jull, AJT, Burr, GS, McHargue, LR, Lange, TE, Lifton, NA, Beck, JW, Donahue, DJ, Lal, D. 2004. New frontiers in dating of geological, paleoclimatic and anthropological applications using accelerator mass spectrometric measurements of 14C and 10Be in diverse samples. Global and Planetary Change 41(3–4):309–323.Google Scholar
Keeling, CI, Nelson, DE, Slessor, KN. 1999. Stable carbon isotope measurement of the carboxyl carbons in bone collagen. Archaeometry 41(1):151–164.Google Scholar
Kheirbeik, L, Hatté, C, Balesdent, J. 2016. Labelled microbial culture as a calibration medium for 13C-isotope measurement of derivatized compounds: application to tert-butyldimethylsilyl amino acids. Rapid Communications in Mass Spectrometry 30(18):1991–2001.Google Scholar
Kleber, M, Sollins, P, Sutton, R. 2007. A conceptual model of organo-mineral interactions in soils: self-assembly of organic molecular fragments into zonal structures on mineral surfaces. Biogeochemistry 85(1):9–24.CrossRefGoogle Scholar
Kramer, AW, Doane, TA, Horwath, WR, van Kessel, C. 2002. Short-term nitrogen-15 recovery vs. long-term total soil N gains in conventional and alternative cropping systems. Soil Biology and Biochemistry 34(1):43–50.Google Scholar
Marsh, K, Mulvaney, R, Sims, GK. 2003. A technique to recover tracer as carboxyl-carbon and alpha-nitrogen from amino acids in soil hydrolysates. Journal of AOAC International 86(6):1106–1111.Google Scholar
Mengel, K. 1996. Turnover of organic nitrogen in soils and its availability to crops. Plant and Soil 181(1):83–93.Google Scholar
Reimer, PJ, Brown, TA, Reimer, RW. 2004. Discussion: reporting and calibration of post-bomb 14C data. Radiocarbon 46(3):1299–1304.Google Scholar
Savidge, WB, Blair, NE. 2005. Intramolecular carbon isotopic composition of monosodium glutamate: biochemical pathways and product source identification. Journal of Agricultural and Food Chemistry 53(2):197–201.Google Scholar
Schmidt, MWI, Torn, MS, Abiven, S, Dittmar, T, Guggenberger, G, Janssens, IA, Kleber, M, Kögel-Knaber, I, Lehmann, J, Manning, DAC, Nannipieri, P, Rasse, DP, Weiner, S, Trumbore, SE. 2011. Persistence of soil organic matter as an ecosystem property. Nature 478(7367):49–56.Google Scholar
Schulten, H-R, Schnitzer, M. 1998. The chemistry of soil organic nitrogen: a review. Biology and Fertility of Soils 26(1):1–15.CrossRefGoogle Scholar
Senwo, ZN, Tabatabai, MA. 1998. Amino acid composition of soil organic matter. Biology and Fertility of Soils 26(3):235–242.Google Scholar
Stevenson, FJ. 1982. Origin and Distribution of Nitrogen in Soil. Agronomy Monograph - Nitrogen in Agricultural Soils. American Society of Agronomy, Crop Science Society of America, Soil Science Society of America. p 1–42.Google Scholar
Takebayashi, Y, Koba, K, Sasaki, Y, Fang, Y, Yoh, M. 2010. The natural abundance of 15N in plant and soil-available N indicates a shift of main plant N resources to NO3 - from NH4 + along the N leaching gradient. Rapid Communications in Mass Spectrometry 24(7):1001–1008.Google Scholar
Tisnérat-Laborde, N, Valladas, H, Kaltnecker, E, Arnold, M. 2003. AMS radiocarbon dating of bones at LSCE. Radiocarbon 45(3):409–419.CrossRefGoogle Scholar
Vieublé Gonod, L, Jones, DL, Chenu, C. 2006. Sorption regulates the fate of the amino acids lysine and leucine in soil aggregates. European Journal of Soil Science 57(3):320–329.Google Scholar