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Dormancy breakage and germination are tightly controlled by hypoxic submergence water on Echinochloa crus-galli seeds from an accession resistant to anaerobic germination

Published online by Cambridge University Press:  14 April 2020

Juliana Echeverry Holguín ,
María Crepy ,
Gustavo G. Striker  and
Federico P.O. Mollard Open the ORCID record for Federico P.O. Mollard [Opens in a new window]
Juliana Echeverry Holguín
Affiliation:
Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina
María Crepy
Affiliation:
INTA Concepción del Uruguay-CONICET, Concepción del Uruguay, Entre Ríos, Argentina
Gustavo G. Striker
Affiliation:
Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina IFEVA, Universidad de Buenos Aires-CONICET, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Hwy, Crawley, WA6009, Australia
Federico P.O. Mollard*
Affiliation:
Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina IFEVA, Universidad de Buenos Aires-CONICET, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina
*
Correspondence: Federico P.O. Mollard, E-mail: fmollard@ifeva.edu.ar
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Abstract

In wetlands, dormancy may be a key functional trait enabling seeds to avoid underwater germination, which could be lethal for seedling establishment. Our objectives were to find out (i) if shallow dormant (i.e. conditionally dormant) Echinochloa crus-galli seeds from an anaerobic germination resistant accession can break dormancy under hypoxic submergence and (ii) if underwater germination can be restored in scarified, non-dormant seeds. Shallow dormant E. crus-galli seeds perceived diurnally alternating temperatures (AT) and red light (R) pulses (i.e. dormancy-breaking cues) under hypoxic submergence; however, an inhibitory far-red light pulse given at the end of the 4-d inundation period demonstrated that most of the seeds (85%) were unable to break dormancy. Scarified E. crus-galli seeds, which did not express dormancy under drained conditions, were unable to germinate under hypoxic submergence, despite being exposed to dormancy-breaking cues (AT + R). Lastly, the temporal window for germination sensitivity to the inhibitory action of hypoxia, once dormancy-breaking signals have been applied, is progressively lost and bounded to approximately 18 h for half of the seed lot. These results highlight the importance of dormancy as a trait enabling E. crus-galli seeds to avoid underwater germination, a risky scenario for seedling emergence and establishment in this facultative hydrophyte.

Keywords

Barnyard grassdiurnally alternating temperaturefloodinghypoxiaphytochromeseed dormancywetlands
Type
Research Paper
Information
Seed Science Research , Volume 30 , Special Issue 4: Functional Seed Ecology , December 2020 , pp. 262 - 267
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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