Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-26T02:57:16.589Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of pre-storage hydration treatments on germination performance, moisture content, DNA synthesis and controlled deterioration tolerance of tomato (Lycopersicon esculentum Mill.) seeds

Published online by Cambridge University Press:  19 September 2008

Jaap G. van Pijlen ,
Steven P. C. Groot ,
H. Lieke Kraak ,
Jan H. W. Bergervoet  and
Raoul J. Bino
Jaap G. van Pijlen
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
Steven P. C. Groot*
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
H. Lieke Kraak
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
Jan H. W. Bergervoet
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
Raoul J. Bino
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
*
*Correspondnce
Get access Rights & Permissions [Opens in a new window]

Abstract

Storage of tomato seeds under deteriorating conditions reduced the rate of germination, the uniformity of germination, total germination and the proportion of normal seedlings. Pre-storage humidification and hydropriming of seeds resulted in a significant increase in resistance to deterioration. In contrast, osmoprimed seeds were more deterioration sensitive. Humidification or hydropriming for one day did not allow nuclei to enter the S phase of the cell cycle. Osmopriming strongly increased the percentage of nuclei with replicated DNA in the embryonic root tip, indicating initiation of the cell cycle and progression towards the G2 phase. The interaction between the prestorage treatments, cell cycle progression and deterioration resistance, is discussed. Based on the changes in moisture content equilibrium and cell cycle activity it is hypothesized that the beneficial effects of pre-storage humidification and hydropriming were related to metabolic activities induced by the partial hydration and that the adverse effects of osmopriming were caused by a decrease in DNA repair activity due to progression in the cell cycle.

Keywords

DNA replicationgerminationhydration treatmentshysteresismoisture contentrelative humidityseed deteriorationseed storagetomato seed
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 6 , Issue 2 , June 1996 , pp. 57 - 63
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alvarado, A.D. and Bradford, K.J. (1988) Priming and storage of tomato seeds (Lycopersicon esculentum Mill.) seeds. 1. Effect of storage temperature on germination rate and viability. Seed Science and Technology 16, 601–612.Google Scholar
Ashraf, M. and Bray, C.M. (1993) DNA synthesis in osmoprimed leek (Allium porrum L.) seeds and evidence of repair and replication. Seed Science Research 3, 15–23.CrossRefGoogle Scholar
Basu, R.M. (1994) An appraisal of research on wet and dry physiological seed treatments and their applicability with special references to tropical and sub-tropical countries. Seed Science and Technology 22, 107–126Google Scholar
Bekendam, J., van Pijlen, J.G. and Kraak, H.L. (1987) The effect of priming on the rate and uniformity of germination of endive seed. Acta Horticulturae 215, 209–218.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994) Seeds: Physiology of development and germination (2nd edition) New York, Plenum Press.CrossRefGoogle Scholar
Bino, R.J., De Vries, J.N., Kraak, H.L. and van Pijlen, J.G. (1992) Flow cytometric determinations of nuclear replication stages in tomato seeds during priming and germination. Annals of Botany 72, 231–236.CrossRefGoogle Scholar
Bino, R.J., Lanteri, S., Verhoeven, H.A. and Kraak, H.L. (1993) Flow cytometric determinations of nuclear replication stages in seed tissues. Annals of Botany 72, 181–187.CrossRefGoogle Scholar
Burgass, R.W. and Powell, A.A. (1984) Evidence for repair processes in invigoration of seeds. Annals of Botany 53, 753–757.CrossRefGoogle Scholar
Cheah, K.S.E. and Osborne, D.J. (1978) DNA lesions occur with loss of viability in embryos of ageing rye seeds. Nature 272, 593–599.CrossRefGoogle Scholar
Chen, C.C. and Morey, R.V. (1989) Equilibrium relative humidity (ERH) relationship for yellow-dent corn. Transactions of the ASEA 32, 999–1006.CrossRefGoogle Scholar
De Castro, R.D., Zheng, X., Bergervoet, J.H.W., De Vos, C.H.R. and Bino, R.J. (1995) β-Tubulin accumulation and DNA replication in imbibing tomato seeds. Plant Physiology 109, 499–504.CrossRefGoogle ScholarPubMed
Elder, R.H. and Osborne, D.J. (1993) Function of DNA synthesis and DNA repair in the survival of embryos during early germination and in dormancy. Seed Science Research 3, 43–53.CrossRefGoogle Scholar
Ibrahim, A.E., Roberts, E.H. and Murdoch, A.J. (1983) Viability of lettuce seeds. II. Survival and osmotically controlled storage. Journal of Experimental Botany 34, 631–640.CrossRefGoogle Scholar
ISTA (1993) International rules for seed testing. Seed Science and Technology 21, 25–30, 37–41.Google Scholar
Justice, O.L. and Bass, L.N. (1978) Principles and practices of seed storage. Agricultural Handbook 506. Washington, USA, US Government Printing Office.Google Scholar
Lanteri, S., Kraak, H.L., De Vos, C.H.R. and Bino, R.J. (1993) Effects of osmotic preconditioning on nuclear replication activity in seeds of pepper (Capsicum annuum L.). Physiologia Plantarum 89, 433–440.CrossRefGoogle Scholar
Liu, Y., Bergervoet, J.H.W., de Vos, C.H., Hilhorst, H.W.M., Kraak, H.L., Karssen, CM. and Bino, R.J. (1994) Nuclear replication activities during imbibition of abscisic acid–and gibberellin deficient tomato (Lycopersicon esculentum Mill.) seeds. Planta 194, 368–373.CrossRefGoogle Scholar
Michel, B.E. and Kaufmann, M.R. (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiology 51, 914–916.CrossRefGoogle ScholarPubMed
Mitra, R. and Basu, R.M. (1979) Seed treatment for viability, vigor and productivity of tomato. Scientia Horticulturae 11, 368–373.CrossRefGoogle Scholar
Murray, A.W. (1992) Creative blocks: cell–cycle checkpoints and feedback controls. Nature 359, 599–604.CrossRefGoogle ScholarPubMed
Nellist, M.E. and Hughes, M. (1973) Physical and biological processes in the drying of seed. Seed Science and Technology 1, 613–643.Google Scholar
Osborne, D.J. (1982) Deoxyribonucleic acid integrity and repair in seed germination: the importance on viability and survival. pp 435–463 in Khan, A.A. (Ed.). The physiology and biochemistry of seed development and germination. Amsterdam, Elsevier.Google Scholar
Petruzelli, L. (1986) Wheat viability at high moisture content under hermetic and aerobic storage conditions. Annals of Botany 58, 259–265.CrossRefGoogle Scholar
Redfearn, M., Clarke, N.A., Osborne, D.J., Halmer, P. and Thomas, T.H. (1995) Nucleic acid integrity in relation to seed vigour in sugar beet. Book of Abstracts, Fifth International Workshop on Seeds,11–15 September 1995,Reading.Google Scholar
Saracco, E, Bino, R.J., Bergervoet, J.W.H. and Lanteri, S. (1995) Influence of priming-induced nuclear replication activity on storability of pepper (Capsicum annuum L.) seed. Seed Science Research 5, 25–29.CrossRefGoogle Scholar
Savino, G., Haigh, P.M. and De Leo, P. (1979) Effects of presoaking upon seed vigour and viability during storage. Seed Science and Technology 7, 57–64.Google Scholar
Saxena, P.K. and King, J. (1989) Isolation of nuclei and their transplantation into plant protoplasts, pp 328–342 in Bajaj, E.P.S. (Ed.). Biotechnology in agriculture and forestry. Plant protoplasts and genetic engineering II. New York, USA, Springer Verlag.Google Scholar
Sybenga, J. (1972) General cytogenetics. Amsterdam, Holland, North-Holland Publishing Company.Google Scholar
Weges, R. (1987) Physiological analysis of methods to relieve dormancy of lettuce seeds. Thesis. Wageningen Agricultural University, Netherlands.Google Scholar