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The impact of genetics and environment on the polar fraction metabolome of commercial Brassica napus seeds: a multi-site study

Published online by Cambridge University Press:  05 August 2019

Djawed Bennouna Open the ORCID record for Djawed Bennouna [Opens in a new window] ,
Jean-Christophe Avice ,
Clément Rosique ,
Ljubica Svilar ,
Célia Pontet ,
Jacques Trouverie ,
Frédéric Fine ,
Xavier Pinochet ,
Karl Fraser  and
Jean-Charles Martin
Djawed Bennouna
Affiliation:
Aix Marseille Université, INSERM, INRA, C2VN, BioMeT, Marseille, France
Jean-Christophe Avice
Affiliation:
Normandie Université, UNICAEN, INRA, SFR Normandie Végétal (FED4277), UMR 950 Ecophysiologie Végétale et Agronomie, F-14032 Caen, France
Clément Rosique
Affiliation:
Aix Marseille Université, INSERM, INRA, C2VN, BioMeT, Marseille, France
Ljubica Svilar
Affiliation:
Aix Marseille Université, INSERM, INRA, C2VN, BioMeT, Marseille, France CriBioM, Criblage Biologique Marseille, Faculté de Médecine de la Timone, Marseille, France
Célia Pontet
Affiliation:
Terres Inovia, Paris, France
Jacques Trouverie
Affiliation:
Normandie Université, UNICAEN, INRA, SFR Normandie Végétal (FED4277), UMR 950 Ecophysiologie Végétale et Agronomie, F-14032 Caen, France
Frédéric Fine
Affiliation:
Terres Inovia, Paris, France
Xavier Pinochet
Affiliation:
Terres Inovia, Paris, France
Karl Fraser
Affiliation:
Food Nutrition & Health Team, Food & Bio-Based Products Group, AgResearch Grasslands Research Centre, Palmerston North, New Zealand Riddet Institute, Massey University, Palmerston North 4442, New Zealand
Jean-Charles Martin*
Affiliation:
Aix Marseille Université, INSERM, INRA, C2VN, BioMeT, Marseille, France
*
*Author for correspondence: Jean-Charles Martin, Email: jean-charles.martin@univ-amu.fr
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Abstract

This study was designed to elucidate the biological variation in expression of many metabolites due to environment, genotype, or both, and to investigate the potential utility of metabolomics to supplement compositional analysis for the design of a new resilient cultivar of Brassica napus that can be steady in phytochemicals in different regions in France. Eight rapeseed varieties, grown in eight regions of France, were compared using a non-targeted metabolomics approach. The statistical analysis highlighted the distance and closeness between the samples in terms of both genotypes and geographical regions. A major environmental impact was observed on the polar metabolome, with different trends, depending on the varieties. Some varieties were very sensitive to the environment, while others were quite resilient. The identified secondary metabolites were mapped into the KEGG pathway database to reveal the most sensitive target proteins susceptible to environmental influences. A glucosyl-transferase encoded by the UGT84A1 gene involved in the biosynthesis of phenylpropanoid was identified. This protein could be rate limiting/promoting in this pathway depending on environmental conditions. The metabolomics approach used in this study demonstrated its efficiency to characterize the environmental influence on various cultivars of Brassica napus seeds and may help identify targets for crop improvement.

Keywords

Brassica napusdata processingenvironmentliquid chromatographymass spectrometrymetabolomics
Type
Research Paper
Information
Seed Science Research , Volume 29 , Issue 3 , September 2019 , pp. 167 - 178
Copyright
Copyright © Cambridge University Press 2019 

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