Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-27T02:02:58.408Z Has data issue: false hasContentIssue false
  • English
  • Français

Osmo-priming in tomato seeds down-regulates genes associated with stress response and leads to reduction in longevity

Published online by Cambridge University Press:  29 July 2021

Ana C.P. Petronilio Open the ORCID record for Ana C.P. Petronilio [Opens in a new window] ,
Thiago B. Batista Open the ORCID record for Thiago B. Batista [Opens in a new window]  and
Edvaldo A. Amaral da Silva Open the ORCID record for Edvaldo A. Amaral da Silva [Opens in a new window]
Ana C.P. Petronilio
Affiliation:
Department of Crop Science, College of Agricultural Sciences, Sao Paulo State University (UNESP), Botucatu, São Paulo, Brazil
Thiago B. Batista
Affiliation:
Department of Crop Science, College of Agricultural Sciences, Sao Paulo State University (UNESP), Botucatu, São Paulo, Brazil
Edvaldo A. Amaral da Silva*
Affiliation:
Department of Crop Science, College of Agricultural Sciences, Sao Paulo State University (UNESP), Botucatu, São Paulo, Brazil
*
*Correspondence: Edvaldo A. Amaral da Silva, E-mail: amaral.silva@unesp.br
Get access Rights & Permissions [Opens in a new window]

Abstract

Tomato seeds subjected to osmo-priming show fast and more uniform germination. However, osmo-priming reduces seed longevity, which is a complex seed physiological attribute influenced by several mechanisms, including response to stress. Thus, to have new insights as to why osmo-primed tomato seeds show a short life span, we performed a transcript analysis during their priming. For that, we performed gene expression studies of the heat-shock protein family genes that were previously reported to be associated with the enhancement of longevity in primed tomato seeds. Physiological assays of germination, vigour and longevity tests were used to support the data. The results show that the short life span of osmo-primed tomato seeds is related to the decrease in the expression of transcripts associated with response to stress during the priming treatment. These results are important because they add information regarding which seed longevity mechanisms are impacted by the priming treatment. In parallel, it will allow the use of these genes as markers to monitor longevity in osmo-primed tomato seeds.

Keywords

conservationprimed seedsSolanum lycopersicumstress response
Type
Research Paper
Information
Seed Science Research , Volume 31 , Issue 3 , September 2021 , pp. 211 - 216
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors have contributed equally to this study.

References

Anese, S, da Silva, EAA, Davide, AC, Rocha Faria, JM, Soares, GCM, Matos, ACB and Toorop, PE (2011) Seed priming improves endosperm weakening, germination, and subsequent seedling development of Solanum lycocarpum St. Hil. Seed Science and Technology 39, 125–139.10.15258/sst.2011.39.1.11CrossRefGoogle Scholar
Batista, TB, Fernandez, JG, da Silva, TA, Maia, J and da Silva, EAA (2020) Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment. AoB Plants 12, 1–10.Google Scholar
Bewley, JD, Bradford, KJ, Hilhorst, HWM and Nonogaki, H (2013) Seeds: physiology of development, germination and dormancy. New York, Springer.10.1007/978-1-4614-4693-4CrossRefGoogle Scholar
Bradford, KJ (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Horticulture Science 21, 1105–1112.Google Scholar
Bruggink, GT, Ooms, JJJ and van der Toorn, P (1999) Induction of longevity in primed seeds. Seed Science Research 9, 49–53.CrossRefGoogle Scholar
Buitink, J, Hemminga, MA and Hoekstra, FA (2000) Is there a role for oligosaccharides in seed longevity? An assessment of intracellular glass stability. Plant Physiology 122, 1217–1224.10.1104/pp.122.4.1217CrossRefGoogle Scholar
Chen, F and Bradford, KJ (2000) Expression of an expansin is associated with endosperm weakening during tomato seed germination. Plant Physiology 124, 1265–1274.10.1104/pp.124.3.1265CrossRefGoogle ScholarPubMed
Chen, F, Dahal, P and Bradford, KJ (2001) Two tomato expansin genes show divergent expression and localization in embryos during seed development and germination. Plant Physiology 127, 928–936.10.1104/pp.010259CrossRefGoogle ScholarPubMed
Ellis, RH and Roberts, EH (1980) Improved equations for the prediction of seed longevity. Annals of Botany 45, 13–30.10.1093/oxfordjournals.aob.a085797CrossRefGoogle Scholar
Fan, F, Yang, X, Cheng, Y, Kang, Y and Chai, X (2017) The DnaJ gene family in pepper (Capsicum annuum L.): comprehensive identification, characterization and expression profiles. Frontiers in Plant Science 8, 689.10.3389/fpls.2017.00689CrossRefGoogle ScholarPubMed
Guo, M, Liu, J, Ma, X, Luo, D, Gong, Z and Lu, M (2016) The plant heat stress transcription factors (HSFs): structure, regulation, and function in response to abiotic stresses. Frontiers in Plant Science 7, 1–13.CrossRefGoogle ScholarPubMed
Gurusinghe, S, Powell, ALT and Bradford, KJ (2002) Enhanced expression of BiP is associated with treatment that extend storage longevity of primed tomato seeds. Journal of the American Society for Horticultural Science 127, 528–534.10.21273/JASHS.127.4.528CrossRefGoogle Scholar
Kaur, H, Petla, BP, Kamble, NU, Singh, A, Rao, V, Salvi, P, Ghosh, S and Majee, M (2015) Differentially expressed seed aging responsive heat shock protein OsHSP18.2 implicates in seed vigor, longevity and improves germination and seedling establishment under abiotic stress. Frontiers in Plant Science 6, 713.10.3389/fpls.2015.00713CrossRefGoogle ScholarPubMed
Kaur, H, Petla, BP and Majee, M (2016) Small heat shock proteins: roles in development, desiccation tolerance and seed longevity, pp. 3–18 in Asea, A; Kaur, P and Calderwood, S (Eds) Heat shock proteins and plants. Cham, Springer International Publishing Switzerland.10.1007/978-3-319-46340-7_1CrossRefGoogle Scholar
Joosen, RVL, Kodde, J, Willems, LAJ, Ligterink, W, van der Plas, LHW and Hilhorst, HWM (2010) Germinator: a software package for high-throughput scoring and curve fitting of Arabidopsis seed germination. Plant Journal 62, 148–159.10.1111/j.1365-313X.2009.04116.xCrossRefGoogle ScholarPubMed
Leprince, O, Pellizzaro, A, Berriri, S and Buitink, J (2017) Late seed maturation: drying without dying. Journal of Experimental Botany 68, 827–841.Google ScholarPubMed
Lima, JJP, Buitink, J, Lalanne, D, Rossi, RF, Pelletier, S, da Silva, EAA and Leprince, O (2017) Molecular characterization of the acquisition of longevity during seed maturation in soybean. PLoS ONE 12, 1–25.Google Scholar
Liu, Y, Bino, RJ, van der Burg, WJ, Groot, SPC and Hilhorst, HWM (1996) Effects of osmotic priming on dormancy and storability of tomato (Lycopersicon esculentum Mill.) seeds. Seed Science Research 6, 49–55.CrossRefGoogle Scholar
Nonogaki, H, Gee, OH and Bradford, KJ (2000) A germination-specific endo-β-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds. Plant Physiology 123, 1235–1245.10.1104/pp.123.4.1235CrossRefGoogle ScholarPubMed
Prieto-Dapena, P, Castano, R, Almoguera, C and Jordano, J (2006) Improved resistance to controlled deterioration in transgenic seeds. Plant Physiology 142, 1102–1112.CrossRefGoogle ScholarPubMed
Ruijter, JM, Pfaffl, MW, Zhao, S, Spiess, AN, Boggy, G, Blom, J, Rutledge, RG, Sisti, D, Lievens, A, De Preter, K, Derveaux, S, Hellemans, J and Vandesompele, J (2013) Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications. Methods 59, 32–46.10.1016/j.ymeth.2012.08.011CrossRefGoogle ScholarPubMed
Sano, N and Seo, M (2019) Cell cycle inhibitors improve seed storability after priming treatments. Journal of Plant Research 132, 263–271.10.1007/s10265-018-01084-5CrossRefGoogle ScholarPubMed
Toorop, PE, Van Aelst, AC and Hilhorst, HWM (2008) Endosperm cap weakening and endo-β-mannanase activity during priming of tomato (Lycopersicon esculentum cv. Moneymaker) seeds are initiated upon crossing a threshold water potential. Seed Science Research 8, 483–492.10.1017/S0960258500004451CrossRefGoogle Scholar
Wang, W, He, A, Peng, S, Huang, J, Cui, K and Nie, L (2018) The effect of storage condition and duration on the deterioration of primed rice seeds. Frontiers in Plant Science 9, 1–17.Google ScholarPubMed
Zhang, L, Hu, W, Gao, Y, Pan, H and Zhang, Q (2018) A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis. Genetics and Molecular Biology 41, 3, 649–660.10.1590/1678-4685-gmb-2017-0206CrossRefGoogle ScholarPubMed
Zinsmeister, J, Leprince, O and Buitink, J (2020) Molecular and environmental factors regulating seed longevity. Biochemical Journal 477, 305–323.10.1042/BCJ20190165CrossRefGoogle ScholarPubMed