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Seed development, testa structure and precocious germination of Chinese cabbage (Brassica rapa subsp. pekinensis)

Published online by Cambridge University Press:  19 September 2008

Chengwei Ren  and
J. Derek Bewley
Chengwei Ren
Affiliation:
Department of Botany, University of Guelph, Ontario N1G 2W1, Canada
J. Derek Bewley*
Affiliation:
Department of Botany, University of Guelph, Ontario N1G 2W1, Canada
*
* E-mail dbewley@uoguelph.ca
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Abstract

On the basis of embryo and seed colour, morphology, accumulation of fresh and dry weight, seed development of Chinese cabbage (Brassica rapa subsp. pekinensis) can be divided into 10 discrete but contiguous stages. Precocious germination (PG) occurs in the seeds of a Chinese cabbage mutant on the parent plant mainly during the maturation period (from stage 5 to 8), with either the radicle or the cotyledon protruding out of the testa. All plants of the mutant line produce some PG seeds, but among the seeds, only 18% (maximum) germinate precociously. The developing mutant seeds have higher water contents and lower dry weights than those of the wild-type and are less tolerant of desiccation. The testa structure of Chinese cabbage seed is similar to that of other Brassica species. In developing seeds, it consists of the epidermis, subepidermis, palisade layer and pigmented layers, while in the mature dry seeds, all the cell layers except the palisade layer are crushed into non-cell structures and are stacked on the outer and inner faces of the palisade layer; the aleurone layer is fused to the testa. An alteration in testa structure of the mutant seeds (both non-germinated and precociously-germinated seeds) is probably related to PG. The secondary cell wall materials are less, or not deposited on the radial and basal walls of the palisade cells on the adaxial side of the testa, which could result in a weaker mechanical restraint, thus leading to PG.

Keywords

Brassica rapaChinese cabbageprecocious germinationseed developmenttesta structure
Type
Physiology & Biochemistry
Information
Seed Science Research , Volume 8 , Issue 3 , September 1998 , pp. 385 - 398
Copyright
Copyright © Cambridge University Press 1998

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References

Aung, L.H., Ball, A. and Kushad, M. (1990) Developmental and nutritional aspects of chayote (Sechium edule, Cucurbitaceae). Economic Botany 44, 157–164.CrossRefGoogle Scholar
Balakrishnan, K., Balamohan, T.N., Veerannah, L., Kulasekaran, M. and Shanmughavelu, K.G. (1986) Seed development and maturation in papaya. Progressive Horticulture 18, 68–70.Google Scholar
Bevilacqua, L.R., Roti-Michelozzi, G., Riggio-Bevilacqua, L.R. (1989) Seed dormancy in Vicia loiseleurii. Observations on the seed coat. Bollettino Societa Italiana di Biologia Sperimentale 65, 861–868.Google Scholar
Bewley, J.D. and Black, M. (1994) Seeds: Physiology of development and germination, 2nd edition. New York, Plenum Press.CrossRefGoogle Scholar
Bewley, J.D. and Oliver, M.J. (1992) Desiccation tolerance in vegetative plant tissues and seeds: protein synthesis in relation to desiccation and a potential role for protection and repair mechanisms. pp 141–160in Somero, G.N., Osmond, C.B., Bolis, C.L. (Eds) Water and life. Berlin, Germany, Springer Verlag.CrossRefGoogle Scholar
Bisgrove, S.R., Crouch, M.L. and Fernandez, D.E. (1995) Chimeric nature of precociously germinating Brassica napus embryos: mRNA accumulation patterns. Journal of Experimental Botany 46, 27–33.CrossRefGoogle Scholar
Boesewinkel, F.D. and Bouman, F. (1995) The seed: structure and function. pp 1–23in Kigel, J., Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Bouman, F. (1975) Integument initiation and testa development in some Cruciferae. Botanical Journal, Linnean Society 70, 213–229.CrossRefGoogle Scholar
Chiang, M.S. and Jacob, A. (1992) Inheritance of precocious seed germination on siliques of cabbage. Canadian Journal of Plant Science 72, 911–913.CrossRefGoogle Scholar
Corner, E.J.H. (1976) The seeds of dicotyledons. Vol. 1. Cambridge. UK, Cambridge University Press.Google Scholar
Crouch, M.L. and Sussex, I.M. (1981) Development and storage-protein synthesis in Brassica napus L. embryos in vivo and in vitro. Planta 153, 64–74.CrossRefGoogle ScholarPubMed
Cummings, D.P., Stuthman, D.D. and Green, C.E. (1978) Morphological mutations induced with ethyl methane sulfonate in oats. Journal of Heredity 69, 3–7.CrossRefGoogle Scholar
Dasgupta, S. and Mandal, R.K. (1993) Compositional changes and storage protein synthesis in developing seeds of Brassica campestris. Seed Science and Technology 21, 291–299.Google Scholar
Davis, A.R., Peterson, R.L. and Shuel, R.W. (1988) Vasculature and ultrastructure of the floral and stipular nectaries of Vicia faba (Leguminosae). Canadian Journal of Botany 66, 1435–1448.CrossRefGoogle Scholar
dos Santos, D. and Yamaguchi, M. (1979) Seed-sprouting in tomato fruits. Scientia Horticulturae 11, 131–139.CrossRefGoogle Scholar
Fountain, D.W. and Outred, H.A. (1990) Seed development in Phaseolus vulgaris L. cv. Seminole. II. Precocious germination in late maturation. Plant Physiology 93, 1089–1093.CrossRefGoogle ScholarPubMed
Fowler, D.B. and Downey, R.K. (1970) Lipid and morphological changes in developing rapeseed, Brassica napus. Canadian Journal of Plant Science 50, 233–247.CrossRefGoogle Scholar
Franke, W. and Lagos-Witte, S. (1993) Contributions to the biology of economic plants. 7. On the viviparity and tuber formation of chayote (Sechium edule). Angewandte Botanik 67, 209–212.Google Scholar
Groot, E.P. and van Caeseele, L.A. (1992) The development of the aleurone layer in canola (Brassica napus). Canadian Journal of Botany 71, 1193–1201.CrossRefGoogle Scholar
Groot, S.P.C. and Karssen, C.M. (1992) Dormancy and germination of abscisic acid-deficient tomato seeds: Studies with the sitiens mutant. Plant Physiology 99, 952–958.CrossRefGoogle ScholarPubMed
Gustafsson, A., Hagberg, A., Lundqvist, U. and Persson, G. (1969) A proposed system of symbols for the collection of barley mutants at Svalov. Hereditas 62, 409–414.CrossRefGoogle Scholar
Harris, W.M. (1987) Comparative ultrastructure of developing seed coats of “hard-seeded” and “soft-seeded” varieties of soybean, Glycine max (L.) Merr. Botanical Gazette 148, 324–331.CrossRefGoogle Scholar
Havey, M.J. (1991) Molecular characterization of the interspecific origin of viviparous onion. Journal of Heredity 82, 501–503.CrossRefGoogle Scholar
Havey, M.J. (1993) A putative donor of S–cytoplasm and its distribution among open-pollinated populations of onion. Theoretical and Applied Genetics 86, 128–134.CrossRefGoogle ScholarPubMed
Hilhorst, H.W.M. and Downie, B. (1995) Primary dormancy in tomato (Lycopersicon esculentum cv. Moneymaker): studies with the sitiens mutant. Journal of Experimental Botany 47, 89–97.CrossRefGoogle Scholar
Howell, G. and Prakash, N. (1990) Embryology and reproductive ecology of the Darling Lily, Crinum flaccidum Herbert. Australian Journal of Botany 38, 433–444.CrossRefGoogle Scholar
Hsiao, A.I. and McIntyre, G.I. (1988) Induction of vivipary in Avena fatua. Physiologia Plantarum 73, 128–133.CrossRefGoogle Scholar
Karssen, C.M., Brinkhorst-van der Swan, D.L.C., Breekland, A.E. and Koornneef, M. (1983) Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of Arabidopsis thaliana (L.) Heunh. Planta 157, 158–165.CrossRefGoogle Scholar
Karssen, C.M. and van Loon, L.C. (1992) Probing hormone action in developing seeds by ABA-deficient and - insensitive mutants. pp 43–53in Karssen, C.M., van Loon, L.C., Vreugdenhil, D. (Eds) Current plant science and biotechnology in agriculture — progress in plant growth regulation. London, Kluwer Academic Publishers.Google Scholar
Kermode, A.R. (1990) Regulatory mechanisms involved in the transition from seed development to germination. Critical Reviews in Plant Science 9, 155–159.CrossRefGoogle Scholar
Kermode, A.R. and Bewley, J.D. (1985) The role of maturation drying in the transition from seed development to germination. 1. Acquisition of desiccation-tolerance and germinability during development of Ricinus communis L. seeds. Journal of Experimental Botany 36, 1906–1915.CrossRefGoogle Scholar
Koornneef, M. and Jorna, M.L. (1982) The isolation of abscisic acid (ABA) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of Arabidopsis thaliana (L.) Heynh. Theoretical and Applied Genetics 61, 385–393.CrossRefGoogle ScholarPubMed
Koornneef, M., Habhartm, C.J., Hilhorst, H.W.M. and Karssen, C.M. (1989) In in vivo inhibition of seed development and reserve protein accumulation in recombinants of abscisic acid biosynthesis and responsiveness mutant in Arabidopsis thaliana. Plant Physiology 90, 462–469.CrossRefGoogle ScholarPubMed
Koornneef, M., Reuling, G. and Karssen, C.M. (1984) The isolation and characterization of abscisic acid insensitive mutants of Arabidopsis thaliana. Physiologia Plantarum 61, 377–383.CrossRefGoogle Scholar
Kriz, A.R., Wallace, M.S. and Paiva, R. (1990) Globulin gene expression in embryos of maize viviparous mutants: Evidence for regulation of the G1b1 gene by ABA. Plant Physiology 92, 538–542.CrossRefGoogle Scholar
Leon-Kloosterziel, K.M., Keijzer, C.J. and Koornneef, M. (1994) A seed shape mutant of Arabidopsis that is affected in integument development. Plant Cell 6, 385–392.CrossRefGoogle ScholarPubMed
Li, L.Y. (1949) Vivipary in some Chinese plants. Botanical Gazette 111, 358–359.CrossRefGoogle Scholar
Ma, F.S. and Zheng, Y.J. (1992) Embryo development of Brassica campestris ssp. Pekinensis. Acta Botanica Boreali Occidentalia Sinica 12, 104–110.Google Scholar
Maiti, R.K., Raju, P.S. and Bidinger, F.R. (1985) Studies on germinability and some aspects of pre-harvest physiology of sorghum grain. Seed Science and Technology 13, 27–35.Google Scholar
Manglesdorf, P.C. (1926) The genetics and morphology of some endosperm characters in maize. Connecticut Agricultural Experiment Station Bulletin (New Haven) 279, 513–614.Google Scholar
Maurya, K.R. (1987) A note on viviparous seed germination in jack fruit. Progressive Horticulture 19, 137–138.Google Scholar
McLean, R.C. and Cook, W.R.I. (1941) Plant science formulae. London, UK, MacMillan and Co. Ltd.Google Scholar
Meng, U. (1985) Light and scanning electron microscope studies to assess micro-morphological criteria for differences in sprouting of caryopses of four cultivars and two primitive forms of wheat. Landbauforschung Volkenrode 35, 191–204.Google Scholar
Murray, B.J.K. (1987) The regulation of soybean pod maturation. Dissertation Abstracts International. B, Sciences and Engineering 48, 318B.Google Scholar
Murthy, P.S.S., Babu, M.D. and Prasad, S.S.R. (1986) Viviparous seed germination in rice varieties at Maruteru. International Rice Research Newsletter 11, 3.Google Scholar
Neuffer, M.G., Jones, L. and Zuber, M.S. (1968) The mutation of maize. Madison, Wisconsin, USA, Crop Science Society Of America.CrossRefGoogle Scholar
Norton, G. and Harris, J.F. (1975) Compositional changes in developing rapeseed (Brassica napus L.). Planta 123, 163–174.CrossRefGoogle Scholar
Opena, R.T., Kuo, C.G. and Yoon, J.Y. (1988) Breeding and seed production of Chinese cabbage in the tropics and subtropics. Asian Vegetable Research and Development Centre, Taiwan, AVRDC Publication.Google Scholar
Picciarelli, P., Piaggesi, A. and Lorenzi, R. (1994) ABA metabolite levels in developing embryos of Sechium edule. Advances in Horticultural Science 8, 201–204.Google Scholar
Raghavan, V. (1986) Embryogenesis in angiosperms, a developmental and experimental study. pp 103–114in Barlow, P.W., Green, P.B., Wylie, C.C. (Eds) Developmental and cell biology, Series 17. Cambridge, UK, Cambridge University Press.Google Scholar
Rao, P. and Kamala, T. (1983) Induced vivipary in Brassica. Journal of Nuclear Agriculture and Biology 12, 80–81.Google Scholar
Raven, P.H., Evert, R.F. and Eichhorn, S.E. (1992) Biology of plants (5th edition). New York, Worth Publishers Inc.Google Scholar
Rivin, C.J. and Grudt, T. (1991) Abscisic acid and the developmental regulation of embryo storage proteins in maize. Plant Physiology 95, 358–365.CrossRefGoogle ScholarPubMed
Robertson, D.S. (1955) The genetics of vivipary in maize. Genetics 40, 745–760.CrossRefGoogle ScholarPubMed
Sarla, N. (1990) Occurrence of vivipary in synthesized Brassica carinata and its characterization by soluble proteins and esterases. Seed Science and Technology 18, 805–812.Google Scholar
Sasikala, S. and Kamala, T. (1985) Ethidium bromide (EB) induced mutants in gingelly (Sesamum indicum L.) cultivar ‘Vinayak’. Theoretical and Applied Genetics 70, 338–339.CrossRefGoogle ScholarPubMed
Schonbeck, M.W. and Bewley, J.D. (1981) Responses of the moss Tortula ruralis to desiccation treatments. I. Effects of minimum water content and rates of dehydration and rehydration. Canadian Journal of Botany 59, 2698–2706.CrossRefGoogle Scholar
Schulz, D., Bachthaler, E. and Kunz, U. (1991) Testa structure in Pelargonium zonale seeds. Gartenbau-wissenschaft 56, 118–126.Google Scholar
Sumner, M.J. and van Caeseele, L. (1987) Ovule development in Brassica campestris: a light microscope study. Canadian Journal of Botany 66, 2459–2469.CrossRefGoogle Scholar
Tal, M. and Nevo, Y. (1973) Abnormal stomatal behavior and root resistance and hormonal imbalance in three wilty mutants of tomato. Biochemical Genetics 8, 291–300.CrossRefGoogle ScholarPubMed
Valenti, G.S., Melone, L., Ferro, M. and Bozzini, A. (1989) Comparative studies on testa structure of “hard-seeded” and “soft-seeded” varieties of Lupinus angustifolius L. (Leguminosae) and on mechanisms of water entry. Seed Science and Technology 17, 563–581.Google Scholar
Vaughan, J.G. (1959) The testa of some Brassica seeds of oriental origin. Phytomorphology 9, 107–110.Google Scholar
Vaughan, J.G. and Whitehouse, J.M. (1971) Seed structure and the taxonomy of the Cruciferae. Botanical Journal, Linnean Society 64, 383–409.CrossRefGoogle Scholar
Welbaum, G.E., Tissaoui, T. and Bradford, K.J. (1990) Water relations of seed development and germination in muskmelon (Cucumis melo L.). III. Sensitivity of germination to water potential and abscisic acid during development. Plant Physiology 92, 1029–1037.CrossRefGoogle ScholarPubMed