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Contributions to the electroretinogram of currents originating in proximal retina

Published online by Cambridge University Press:  02 June 2009

Laura J. Frishman ,
Paul A. Sieving  and
Roy H. Steinberg
Laura J. Frishman
Affiliation:
Departments of Physiology and Ophthalmology, University of California, San Francisco
Paul A. Sieving
Affiliation:
Departments of Physiology and Ophthalmology, University of California, San Francisco
Roy H. Steinberg
Affiliation:
Departments of Physiology and Ophthalmology, University of California, San Francisco
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Abstract

We have investigated responses in proximal retina of the cat that contribute to two kinds of electroretinogram (ERG) recordings: (1) the pattern ERG, a light-adapted response and (2) the threshold and near threshold ERG, a dark-adapted response (Sieving et al., 1986a, 1986b; Sieving & Steinberg, 1985). In intraretinal, extracellular recordings, two negative-going responses were identified that are maximal around the inner plexiform layer, and distinct from PII, which is maximal in distal retina: under light-adapted conditions, a spatially tuned response at light onset and light offset, the “M-wave” (previously described in cold-blooded animals by Karwoski & Proenza (1977, 1980)), and under dark-adapted conditions, the scotopic threshold response, or “STR,” a response at light onset. The results under dark-adapted conditions are examined in more detail here.

The STR is a very sensitive response whose threshold is 1.5–2.0 log units below that of the dc-component of PII and therefore well below the threshold of the a-, b-, and c-waves. It saturates about 2.4 log units below rod saturation. The STR contributes a negative-going potential to the dark-adapted ERG that is dominant near threshold; while PII (dc-component and b-wave) contributes a positive-going potential that is dominant at higher intensities (Sieving et al., 1986b). Investigation of the mechanism of the proximal retinal responses that contribute to the ERG supports a K+-Müller cell hypothesis of their origin.

Keywords

ElectroretinogramRetinaCat retinaMüller cellRetinal [K+]o
Type
Research Article
Information
Visual Neuroscience , Volume 1 , Issue 3 , May 1988 , pp. 307 - 315
Copyright
Copyright © Cambridge University Press 1988

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