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Light-induced GTPase activity and GTP[γS] binding in squid retinal photoreceptors

Published online by Cambridge University Press:  02 June 2009

Joel E. Brown ,
Anthony Combs ,
Karen Ackermann  and
Craig C. Malbon
Joel E. Brown
Affiliation:
Department of Ophthalmology and Visual Sciences, Washington University, St. Louis
Anthony Combs
Affiliation:
Department of Ophthalmology and Visual Sciences, Washington University, St. Louis
Karen Ackermann
Affiliation:
Department of Ophthalmology and Visual Sciences, Washington University, St. Louis
Craig C. Malbon
Affiliation:
Department of Pharmacology, Health Sciences Center, SUNY Stony Brook, Stony Brook
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Abstract

Illumination greatly increases the GTPase activity in homogenates of squid (Loligo) whole retinas or rhabdomeric membranes. Adenylylimidodiphosphate inhibits the light-insensitive (but not the light-sensitive) GTPase activity in these homogenates. Illumination also greatly increases the binding of GTP[γS] to the rhabdomeric membranes. This binding at saturating illuminations indicates that there are approximately 10–100 times more rhodopsin molecules than G-protein molecules in squid photoreceptors. Each light-activated rhodopsin molecule activates about 10 G-protein molecules which might provide amplification for the first stage of the phototransduction cascade.

Keywords

Invertebrate photoreceptor(s)PhototransductionGTPase activityGTP[γS] binding
Type
Research Articles
Information
Visual Neuroscience , Volume 7 , Issue 6 , December 1991 , pp. 589 - 595
Copyright
Copyright © Cambridge University Press 1991

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