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Effects of Diphenylether Herbicides on Reactions of Mitochondria and Chloroplasts

Published online by Cambridge University Press:  12 June 2017

D. E. Moreland ,
W. J. Blackmon ,
H. G. Todd  and
F. S. Farmer
D. E. Moreland
Affiliation:
Crops Research Division, Agr. Res. Serv., U. S. Dep. of Agr., Crop Science Department, North Carolina State University, Raleigh, North Carolina
W. J. Blackmon
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, North Carolina
H. G. Todd
Affiliation:
Crops Research Division, Agr. Res. Serv., U. S. Dep. of Agr., Crop Science Department, North Carolina State University, Raleigh, North Carolina
F. S. Farmer
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, North Carolina
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Abstract

Effects of three diphenylether herbicides [2,4-dichlorophenyl-p-nitrophenyl ether (nitrofen); 2,4,6-trichlorophenyl-4′-nitrophenyl ether (hereinafter referred to as MC-1478); and 2,4′-dinitro-4-trifluoromethyl-diphenylether (hereinafter referred to as C-6989)] were measured on phosphorylation and electron transport in spinach (Spinacia oleracea L.) chloroplasts, and mung bean (Phaseolus aureus L., var. Jumbo) and white potato tuber (Solarium tuberosum L.) mitochondria. All of the diphenylethers acted primarily as inhibitors of chloroplast noncyclic electron transport, and the coupled photophosphorylation. The compounds ranked in the following decreasing order of inhibitory effectiveness: MC-1478 ≥ C-6989 >> nitrofen. A site of action close to light reaction II was suggested. At high molar concentrations, marginal interference with cyclic electron transport or phosphorylation was obtained. In mitochondria, MC-1478 and nitrofen acted primarily as electron transport inhibitors with malate, NADH, and succinate as substrates. MC-1478 was a slightly stronger inhibitor than nitrofen. Only slight stimulation of ADP-limited oxygen uptake was obtained during the oxidation of NADH and succinate; whereas, strong inhibition of oxygen uptake was obtained with malate. C-6989 also weakly stimulated ADP-limited oxygen uptake with NADH and succinate but differed from the two chlorinated diphenylethers in that electron transport was not inhibited when ADP was present in excess. Interference with ATP generation could be one of the mechanisms through which the phytotoxicity of diphenylether herbicides is expressed.

Type
Research Article
Information
Weed Science , Volume 18 , Issue 5 , September 1970 , pp. 636 - 642
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. Avron, M. and Neumann, J. 1968. Photophosphorylation in chloroplasts. Annu. Rev. Plant Physiol. 19:137–166.CrossRefGoogle Scholar
2. Baltscheffsky, H. 1963. Inhibition of photophosphorylation in isolated spinach chloroplasts by lower aliphatic straight-chain alcohols. Acta Chem. Scand. 17:S308–S312.CrossRefGoogle Scholar
3. Bonner, W. D. Jr. 1967. A general method for the preparation of plant mitochondria, p. 126–133. In Estabrook, R. W. and Pullman, M. E. (ed.) Methods in Enzymology, vol. 10. Academic Press, New York.Google Scholar
4. Chance, B. and Williams, G. R. 1955. Respiratory enzymes in oxidative phosphorylation. III. The steady state. J. Biol. Chem. 217:409–427.CrossRefGoogle ScholarPubMed
5. Chance, B., Williams, G. R., and Hollunger, G. 1963. Inhibition of electron and energy transfer in mitochondria. III. Spectroscopic and respiratory effects of uncoupling agents. J. Biol. Chem. 278:439–444.CrossRefGoogle Scholar
6. Ferrari, T. E. and Moreland, D. E. 1969. Effects of 3,5-dihalogenated-4-hydroxybenzonitriles on the activity of mitochondria from white potato tubers. Plant Physiol. 44:429–434.CrossRefGoogle ScholarPubMed
7. Gromet-Elhanan, Z. 1968. The inhibition of photoreactions of chloroplasts by ioxynil. Biochem. Biophys. Res. Commun. 30:28–31.CrossRefGoogle ScholarPubMed
8. Gromet-Elhanan, Z. 1968. Energy-transfer inhibitors and electron transport inhibitors in chloroplasts. Arch. Biochem. Biophys. 123:447–456.CrossRefGoogle ScholarPubMed
9. Ikuma, H. and Bonner, W. D. Jr. 1967. Properties of higher plant mitochondria. I. Isolation and some characteristics of tightly-coupled mitochondria from dark-grown mung bean hypocotyls. Plant Physiol. 42:67–75.CrossRefGoogle ScholarPubMed
10. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–275.CrossRefGoogle ScholarPubMed
11. Mason, G. W. and Brooker, E. G. 1968. A new diphenyl ether for weed control in field brassica crops. New Zealand Weed and Pest Cont. Conf. 21:163–172.CrossRefGoogle Scholar
12. Matsunaka, S. 1969. Activation and inactivation of herbicides by higher plants. Residue Rev. 25:45–58.Google Scholar
13. Matsunaka, S. 1969. Acceptor of light energy in photoactivation of diphenylether herbicides. J. Agr. Food Chem. 17:171–175.CrossRefGoogle Scholar
14. Moreland, D. E. and Blackmon, W. J. 1970. Effects of 3,5-dibromo-4-hydroxybenzaldehyde 0-(2,4-dinitrophenyl)oxime on reactions of mitochondria and chloroplasts. Weed Sci. 18: 419–426.CrossRefGoogle Scholar
15. Taussky, H. H. and Shorr, E. 1953. A microcolorimetric method for the determination of inorganic phosphorous. J. Biol. Chem. 202:675–685.CrossRefGoogle Scholar
16. Vernon, L. P. and Avron, M. 1965. Photosynthesis. Annu. Rev. Biochem. 34:269–296.CrossRefGoogle ScholarPubMed
17. Witt, H. T., Döring, G., Rumberg, B., Schmidt-Mende, P., Siggel, U., and Stiehl, H. H. 1967. Electron transport in photosynthesis. Brookhaven Symp. Biol. 19:161–167.Google Scholar