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Evaluating anthranilate synthase as a herbicide target

Published online by Cambridge University Press:  12 June 2017

Daniel L. Siehl ,
Mani V. Subramanian ,
Eric W. Walters ,
Jonathan H. Blanding ,
Thierry Niderman  and
Christian Weinmann
Daniel L. Siehl*
Affiliation:
Novartis Crop Protection, 975 California Avenue, Palo Alto, CA 94304-1104
Mani V. Subramanian
Affiliation:
Novartis Crop Protection, Paro Alto, CA 943041104
Eric W. Walters
Affiliation:
Novartis Crop Protection, Paro Alto, CA 943041104
Jonathan H. Blanding
Affiliation:
Novartis Crop Protection, Paro Alto, CA 943041104
Thierry Niderman
Affiliation:
Novartis Crop Protection, R-1040. P7A, Basel, Switzerland CH-4002
Christian Weinmann
Affiliation:
Novartis Crop Protection, R-1040. P7A, Basel, Switzerland CH-4002
*
dan.siehl@cp.novartis.com.
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Abstract

Attempts to discover new active ingredients and target sites within the aromatic pathway have resulted in the synthesis of potent enzyme inhibitors, but no herbicides. As an aid in identifying a new target for inhibitor design and screening, we have determined the mode of action of a compound (6-methyl anthranilate) that exhibits noncommercial levels of herbicidal activity. Our evidence suggests that 6-methyl anthranilate is converted in vivo, by traversing the tryptophan biosynthetic sequence, to 4-methyl tryptophan, which inhibits anthranilate synthase. Inhibitors synthesized by design and those found by target-based screening converged on analogs of tryptophan and anthranilate. None, however, was more herbicidal than 6-methyl anthranilate.

Keywords

Anthranilate synthase6-methyl anthranilateherbicide targetaromatic biosynthesis
Type
Symposium
Information
Weed Science , Volume 45 , Issue 5 , October 1997 , pp. 628 - 633
Copyright
Copyright © 1997 by the Weed Science Society of America 

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