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Resistance to Glyphosate in Junglerice (Echinochloa colona) from California

Published online by Cambridge University Press:  20 January 2017

Rocío Alarcón-Reverte ,
Alejandro García ,
Jaime Urzúa  and
Albert J. Fischer
Rocío Alarcón-Reverte
Affiliation:
Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616
Alejandro García
Affiliation:
Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616
Jaime Urzúa
Affiliation:
Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616
Albert J. Fischer*
Affiliation:
Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616
*
Corresponding author's E-mail: ajfischer@ucdavis.edu
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Abstract

A suspected glyphosate-resistant (R) junglerice population was collected from a glyphosate-R corn field near Durham in northern California where glyphosate had been applied at least twice a year for over 6 yr. Based on the amount of glyphosate required to reduce growth by 50% (ED50), the R population was 6.6 times more R than the susceptible (S) standard population. Based on the glyphosate concentration that inhibits EPSPS by 50% based on shikimate accumulation (I50) in leaf discs, R plants were four times more R than S plants. By 3 d after treatment with 0.42 kg ae ha−1 glyphosate, the S population had accumulated approximately five times more shikimate than the R population. No differences in [14C]-glyphosate uptake and translocation were detected between R and S plants. However, partial sequencing of the EPSPS gene revealed a mutation in R plants causing a proline to serine change at EPSPS position 106 (P106S). Our results reveal the first case of a P106S target site mutation associated with glyphosate resistance in junglerice.

Keywords

Glyphosatejunglerice, Echinochloa colona (L.) LinkEPSPS5-enolpyruvylshikimate-3-phosphate synthaseshikimate accumulationtarget site mutationglyphosate leaf uptakeglyphosate translocation
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 61 , Issue 1 , March 2013 , pp. 48 - 54
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Current address: Colegio de Postgraduados Campus Montecillo, Montecillo, Estado de México, México. C.P. 56230.

References

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