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Suppression of CtpA in Mouseearcress Produces a Phytotoxic Effect: Validation of CtpA as a Target for Herbicide Development

Published online by Cambridge University Press:  20 January 2017

Yun-Chia Sophia Chen ,
Brad J. Fabbri ,
Claire A. CaJacob ,
John C. Anderson  and
Stephen M. G. Duff
Yun-Chia Sophia Chen
Affiliation:
Monsanto Company, 700 Chesterfield Pkwy North, St. Louis, MO, 63198
Brad J. Fabbri
Affiliation:
Monsanto Company, 700 Chesterfield Pkwy North, St. Louis, MO, 63198
Claire A. CaJacob
Affiliation:
Monsanto Company, 700 Chesterfield Pkwy North, St. Louis, MO, 63198
John C. Anderson
Affiliation:
Monsanto Co, 2111 Piilani Hwy, P.O. Box 629, Kihei, HI 96753
Stephen M. G. Duff*
Affiliation:
Monsanto Company, 700 Chesterfield Pkwy North, St. Louis, MO, 63198
*
Corresponding author's E-mail: stephen.m.duff@monsanto.com
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Abstract

To validate carboxyterminal processing protease of D1 protein (CtpA) as a target for herbicide discovery, CtpA sense mRNAs were overexpressed to suppress the internal level of CtpA protein in mouseearcress plants. Using antibodies raised against recombinant CtpA protein, we demonstrated that we have generated transgenic mouseearcress plants with reduced levels of CtpA protein and plants with elevated levels of CtpA protein. Transgenic plants with severely reduced levels of CtpA protein exhibited a bleached and chlorotic phenotype and stunted growth. The mutant phenotypes were enhanced by bright illumination. However, plants with a slight reduction of CtpA protein did not exhibit the mutant phenotype and could not be distinguished from wild-type plants under normal growth conditions. Several CtpA enzyme inhibitors were shown to have herbicidal activity in planta. Interestingly, plants producing excessive amount of CtpA protein were shown to be resistant to these inhibitors. Our results suggest that CtpA is essential for plant growth and development, but a reduced amount of CtpA is sufficient to carry out its essential function. CtpA may be a good target for herbicide development, but very high levels of inhibition may be required to produce a herbicidal effect. In addition, overexpressing CtpA in target plants might provide a mechanism for producing plants resistant to the herbicide.

Keywords

CtpA, Carboxyterminal processing protease of D1 protein (also referred to as D1 protease)mouseearcress, Arabidopsis thaliana (L.) HeynhD1 proteasecosuppressionphytotoxicityherbicide targetmode of action
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 55 , Issue 4 , August 2007 , pp. 283 - 287
Copyright
Copyright © Weed Science Society of America 

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