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A Trp574 to Leu Amino Acid Substitution in the ALS Gene of Annual Bluegrass (Poa annua) Is Associated with Resistance to ALS-Inhibiting Herbicides

Published online by Cambridge University Press:  20 January 2017

J. Scott McElroy ,
Michael L. Flessner ,
Zhuoyu Wang ,
Fenny Dane ,
Robert H. Walker  and
Glenn R. Wehtje
J. Scott McElroy*
Affiliation:
Department of Agronomy and Soils, 201 Funchess Hall, Auburn University, Auburn, AL 36849
Michael L. Flessner
Affiliation:
Department of Agronomy and Soils, 201 Funchess Hall, Auburn University, Auburn, AL 36849
Zhuoyu Wang
Affiliation:
Department of Horticulture, 101 Funchess Hall, Auburn University, Auburn, AL 36849
Fenny Dane
Affiliation:
Department of Horticulture, 101 Funchess Hall, Auburn University, Auburn, AL 36849
Robert H. Walker
Affiliation:
Department of Agronomy and Soils, 201 Funchess Hall, Auburn University, Auburn, AL 36849
Glenn R. Wehtje
Affiliation:
Department of Agronomy and Soils, 201 Funchess Hall, Auburn University, Auburn, AL 36849
*
Corresponding author's Email: jsm0010@auburn.edu
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Abstract

Annual bluegrass is commonly controlled by acetolactate synthase (ALS)-inhibiting herbicides in managed turfgrass. An annual bluegrass population with suspected resistance to ALS-inhibiting herbicides was collected from Grand National Golf Course in Opelika, AL (GN population). Subsequent testing confirmed resistance of the GN population to foramsulfuron, trifloxysulfuron, bispyribac-sodium (bispyribac), and imazaquin when compared to a susceptible population collected locally at Auburn University (AU population). Sequencing of the ALS gene revealed a point mutation resulting in an amino acid substitution at Trp574. Cloning of the ALS gene surrounding the Trp574 region yielded two distinct ALS gene sequences: one producing Trp574 and one producing Leu574. Trp574 to Leu has been previously correlated with resistance to ALS-inhibiting herbicides. Both AU and GN gene sequences contained other similar silent and missense mutations. This research confirms resistance of annual bluegrass to ALS-inhibiting herbicides with Trp574 to Leu amino acid substitution being the most likely mode of resistance based on past literature.

Keywords

Bispyribacforamsulfuronimazaquintrifloxysulfuronannual bluegrass, Poa annua L.Acetolactate synthase-inhibiting herbicidesherbicide resistanceimidazolinonepyrimidinyl benzoatessulfonylureatarget-site mutationsturfgrass management
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 61 , Issue 1 , March 2013 , pp. 21 - 25
Copyright
Copyright © Weed Science Society of America 

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