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Glyphosate Tolerance Mechanism in Italian Ryegrass (Lolium multiflorum) from Mississippi

Published online by Cambridge University Press:  20 January 2017

Vijay K. Nandula ,
Krishna N. Reddy ,
Daniel H. Poston ,
Agnes M. Rimando  and
Stephen O. Duke
Vijay K. Nandula*
Affiliation:
Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776
Krishna N. Reddy
Affiliation:
USDA-ARS, Southern Weed Science Research Unit, P.O Box 350, Stoneville, MS 38776
Daniel H. Poston
Affiliation:
Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776
Agnes M. Rimando
Affiliation:
USDA-ARS, Natural Products Utilization Research Unit, P.O. Box 8048, University, MS 38677
Stephen O. Duke
Affiliation:
USDA-ARS, Natural Products Utilization Research Unit, P.O. Box 8048, University, MS 38677
*
Corresponding author's E-mail: vknandula@yahoo.com
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Abstract

A threefold glyphosate tolerance was identified in two Italian ryegrass populations, T1 and T2, from Mississippi. Laboratory experiments were conducted to characterize the mechanism of glyphosate tolerance in these populations. The T1 population absorbed less 14C-glyphosate (43% of applied) compared to the susceptible (S) population (59% of applied) at 48 h after treatment (HAT). The T2 population absorbed 14C-glyphosate at levels (56% of applied at 48 HAT) that were similar to both T1 and S populations, but tended to be more comparable to the S population. The amount of 14C-glyphosate that remained in the treated leaf was significantly higher in both T1 (67% of absorbed) and T2 (65% of absorbed) populations compared to the S population (45% of absorbed) at 48 HAT. The amount of 14C-glyphosate that moved out of treated leaf to shoot and root was lower in both T1 (25% of absorbed in shoot and 9% of absorbed in root) and T2 (25% of absorbed in shoot and 11% of absorbed in root) populations compared to the S population (40% of absorbed in shoot and 16% of absorbed in root) at 48 HAT. There were no differences in epicuticular wax mass among the three populations. Treating a single leaf with glyphosate solution at the field use rate (0.84 kg ae ha−1) as 10 1-µl droplets killed the S plant but not the T1 and T2 plants (33 and 55% shoot fresh-weight reduction, respectively). Shikimic acid accumulated rapidly at higher levels in glyphosate-treated leaf segments of the S population compared to the T1 population up to 100 µM glyphosate. However, above 500 µM glyphosate, the levels of shikimate were similar in both the S and T1 populations. Furthermore, shikimic acid content was three- to sixfold more in whole plants of the S population treated with 0.22 kg ae ha−1 glyphosate compared to the T1 and T2 populations. No degradation of glyphosate to aminomethylphosphonic acid was detected among the tolerant and susceptible populations. These results indicate that tolerance to glyphosate in the T1 population is partly due to reduced absorption and translocation of glyphosate and in the T2 population it is partly due to reduced translocation of glyphosate.

Keywords

GlyphosateItalian ryegrass, Lolium multiflorum Lam.Absorptionglyphosate resistanceherbicide resistancemetabolismshikimatetranslocationweed resistance
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 3 , June 2008 , pp. 344 - 349
Copyright
Copyright © Weed Science Society of America 

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