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A Rare Ile-2041-Thr Mutation in the ACCase Gene Confers Resistance to ACCase-inhibiting Herbicides in Shortawn Foxtail (Alopecurus aequalis)

Published online by Cambridge University Press:  08 February 2017

Wenlei Guo ,
Lele Zhang ,
Hengzhi Wang ,
Qi Li ,
Weitang Liu  and
Jinxin Wang
Wenlei Guo
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Lele Zhang
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Hengzhi Wang
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Qi Li
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Weitang Liu
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
Jinxin Wang*
Affiliation:
Graduate Student, Graduate Student, Graduate Student, Graduate Student, Lecturer and Professor, Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai’an 271018, People’s Republic of China
*
*Corresponding author’s E-mail: wangjx@sdau.edu.cn
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Abstract

Understanding the mechanism of herbicide resistance is fundamental for designing sustainable weed control strategies and exploiting herbicides rationally. Shortawn foxtail is a problem grass weed infesting several important crops in China. The repeated use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides has resulted in herbicide resistance in this weed. The ACCase gene of resistant individuals of a shortawn foxtail population (JSLS-1) has an Ile-2041-Thr mutation. F2 generation seeds, originated from the same heterozygous plant, were harvested, and two homozygous mutant (JSLS-1RR) and wild (JSLS-1SS) populations for the Ile-2041-Thr mutation were obtained. In whole plants, the JSLS-1RR population conferred high resistance to fenoxaprop and clodinafop, moderate resistance to haloxyfop, low resistance to pinoxaden, and no obvious resistance to clethodim and sethoxydim, compared with JSLS-1SS and a proven susceptible population (HNXY-1). A derived cleaved amplified polymorphic sequence (dCAPS) marker was developed to rapidly detect the rare Ile-2041-Thr mutation in the shortawn foxtail population. This is the first report of the cross-resistance pattern of Ile-2041-Thr mutation, and the robust dCAPS marker could quickly detect this mutation in shortawn foxtail.

Keywords

Clethodimclodinafopfenoxaprophaloxyfoppinoxadensethoxydimshortawn foxtail, Alopecurus aequalis Sobol.ACCasecross-resistancedCAPSgene mutationresistance mechanism
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 65 , Issue 2 , March 2017 , pp. 239 - 246
Copyright
© Weed Science Society of America, 2017 

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Footnotes

Associate Editor: Franck E. Dayan, Colorado State University

References

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