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Target-site mutation and enhanced metabolism confer resistance to thifensulfuron-methyl in a multiple-resistant redroot pigweed (Amaranthus retroflexus) population

Published online by Cambridge University Press:  14 December 2020

Yi Cao ,
Shouhui Wei ,
Hongjuan Huang ,
Wenyu Li ,
Chaoxian Zhang  and
Zhaofeng Huang Open the ORCID record for Zhaofeng Huang [Opens in a new window]
Yi Cao
Affiliation:
Master’s Degree Student, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Shouhui Wei
Affiliation:
Associate Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Hongjuan Huang
Affiliation:
Associate Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Wenyu Li
Affiliation:
Research Assistant, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Chaoxian Zhang
Affiliation:
Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Zhaofeng Huang*
Affiliation:
Associate Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
*
Author for correspondence: Zhaofeng Huang, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing100193, China. (Email: huangzhaofeng666@163.com)
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Abstract

Redroot pigweed (Amaranthus retroflexus L.) is a troublesome dicot weed species widely distributed across China. A population of A. retroflexus that survived the recommended label rate of thifensulfuron-methyl was collected from the main soybean [Glycine max (L.) Merr.] production area in China. Whole-plant dose–response assays indicated that the resistant (R) population was highly resistant (61.80-fold) to thifensulfuron-methyl compared with the susceptible (S1 and S2) populations. In vitro acetolactate synthase (ALS) activity experiments showed that the thifensulfuron-methyl I50 value for the R population was 40.17 times higher than that for the S1 population. A preliminary malathion treatment study indicated that the R population might have cytochrome P450–mediated metabolic resistance. The R population exhibited a high level of cross-resistance to representative ALS herbicides (imazethapyr, flumetsulam, and bispyribac-sodium) and multiple resistance to the commonly used protoporphyrinogen oxidase (PPO)-inhibiting herbicides lactofen and fomesafen. Two common mutations, Trp-574-Leu in ALS and Arg-128-Gly in PPO2, were identified within the R population. This study identified possible enhanced metabolism of thifensulfuron-methyl coexisting with target-site mutations in both ALS and PPO2 in a multiple-resistant A. retroflexus population.

Keywords

ALS activitymetabolismmultiple resistanceredroot amaranth
Type
Research Article
Information
Weed Science , Volume 69 , Issue 2 , March 2021 , pp. 161 - 166
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of Weed Science Society of America

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Footnotes

Associate Editor: Christopher Preston, University of Adelaide

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