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Amitrole Interference with Adenine-2-14C Metabolism by Saccharomyces cerevisiae

Published online by Cambridge University Press:  12 June 2017

J. L. Hilton  and
P. C. Kearney
J. L. Hilton
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, Maryland
P. C. Kearney
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, Maryland
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Abstract

Metabolic incorporation of adenine-2-14C by Saccharomyces cerevisiae was examined for alterations produced by 3-amino-1,2,4-triazole (amitrole) and histidine. The radioactivity recovered from cells after a 4-hour incubation was found predominantly in ethanol-insoluble components (nucleic acids and proteins) of untreated cultures. Amitrole inhibited incorporation of 14C into insoluble components. Carbon-labeled imidazoleglycerol phosphate, a precursor of histidine, and labeled imidazoleglycerol accumulated in the ethanol-soluble fraction of amitrole treated cells. Imidazoleglycerol accumulated in the external medium. Histidine prevented 14C incorporation into the hot trichloroacetic acid-insoluble residue (protein), prevented amitrole-induced imidazoleglycerol and imidazoleglycerol phosphate accumulation, and circumvented amitrole inhibition of 14C incorporation into the hot trichloroacetic acid-soluble (nucleic acids) fraction.

Type
Research Article
Information
Weeds , Volume 13 , Issue 1 , January 1965 , pp. 22 - 25
Copyright
Copyright © 1965 Weed Science Society of America 

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References

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