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A Rapid Method for Enumeration of Viable Seeds in Soil

Published online by Cambridge University Press:  12 June 2017

Charles R. Malone
Charles R. Malone*
Affiliation:
Department of Zoology, Rutgers-The State University, New Brunswick, New Jersey
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Abstract

Soil dispersion in an aqueous solution of sodium hexametaphosphate and sodium bicarbonate facilitates extraction of seeds by flotation with megnesium sulphate. The percentage viability of seeds collected in this manner subsequently can be determined with 2,3,5-triphenyl-tetrazolium chloride. Extraction of seeds usually is 100% efficient and enumeration of viable seeds via the tetrazolium test is accomplished much more rapidly than with conventional methods.

Type
Brief Papers
Information
Weeds , Volume 15 , Issue 4 , October 1967 , pp. 381 - 382
Copyright
Copyright © 1967 Weed Science Society of America 

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References

Literature Cited

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