Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-19T15:49:17.574Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of 2,4-Dichlorophenoxyacetic Acid on the Nucleic Acid and Protein Content of Seedling Tissue

Published online by Cambridge University Press:  12 June 2017

S. H. West ,
J. B. Hanson  and
J. L. Key
S. H. West
Affiliation:
Agricultural Research Service, U.S.D.A., Univ. of Florida, Gainesville
J. B. Hanson
Affiliation:
Agricultural Research Service, U.S.D.A., Univ. of Florida, Gainesville
J. L. Key
Affiliation:
Dept. or Agronomy, Univ. of Illinois, Urbana
Get access

Extract

One of the most marked effects of herbicidal concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) is the swelling and proliferation of basal stem tissues. Sell et al. reported a large increase in the protein content of stems of bean plants treated with 2,4-D. Shaw, et al. observed an increase of total protein in plants treated with 2,4-D and Rebstock, et al. found the nucleic acid content to double in the stems of bean plants treated with 2,4-D. Rebstock et al. postulated that nucleic acid was involved in the unusual growth and development of the plant. Skoog has presented an attractive hypothesis linking auxin action with nucleic acid metabolism. Current biochemical investigations leave little doubt that RNA (ribonucleic acid), particularly of the microsome fraction of the cytoplasm, is involved in protein synthesis. Furthermore, RNA appears to be implicated in oxidative phosphorylation and ion absorption, two processes known to be affected by 2,4-D.

Type
Research Article
Information
Weeds , Volume 8 , Issue 3 , July 1960 , pp. 333 - 340
Copyright
Copyright © 1960 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Bonner, J. Protein synthesis and the control of plant processes. Amer. Jour. Bot. 46:58–62. 1959.CrossRefGoogle Scholar
2. Christiansen, G. S., and Thimann, K. V. The metabolism of stem tissue during growth and its inhibition. III. Nitrogen metabolism. Arch. Biochem. 28:117–129. 1950.Google Scholar
3. Clark, J. M. Jr. Amino acid activation in plant tissues. Jour. Biol. Chem. 233:421–424. 1958.Google Scholar
4. Cooke, A. R. Influence of 2,4-D on the uptake of minerals from the soil. Weeds 5:25–28. 1957.CrossRefGoogle Scholar
5. Ginsburg, V., Stumpf, P. K., and Hassid, W. Z. The isolation of uridine diphosphate derivatives of D-glucose, D-galactose, D-xylose, and L-arabinose from young bean seedlings. Jour. Biol. Chem. 223:977–983. 1956.Google Scholar
6. Hanson, J. B. The effect of ribonuclease on oxidative phosphorylation by mitochondria. Jour. Biol. Chem. 234:1303–1306. 1959.CrossRefGoogle ScholarPubMed
7. Kennedy, E. P. Metabolism of Lipides. Ann. Rev. Biochem. 26:119–148. 1957.CrossRefGoogle ScholarPubMed
8. Lansing, A. I., and Rosenthal, T. B. The relation between ribonucleic acid and ionic transport across the cell surface. Jour. Cell. Comp. Physiol. 40:337–340. 1953.Google Scholar
9. Laties, G. G. Respiration and cellular work and the regulation of the respiration rate in plants. Survey Biol. Progress 3:215–299. 1957.Google Scholar
10. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. Protein measurement with the Folin phenol reagent. Jour. Biol. Chem. 193:265–275. 1951.Google Scholar
11. Lund, H. A., Vatter, A. E., and Hanson, J. B. Biochemical and cytological changes accompanying growth and differentiation in the roots of Zea mays. Jour. Biophys. Biochem. Cytol. 4:87–98. 1958.CrossRefGoogle ScholarPubMed
12. Nance, J. F. Inhibition of salt accumulation in excised wheat roots by 2,4-dichlorophenoxyacetic acid. Science 109:174–176. 1949.Google Scholar
13. Ogur, M., and Rosen, G. The extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Arch. Biochem. 25:262–276. 1950.Google Scholar
14. Rebstock, T. L., Hamner, C. L., and Sell, H. M. The influence of 2,4-dichlorophenoxyacetic acid on the phosphorus metabolism of cranberry bean plants (Phaseolus vulgaris). Plant Physiol. 29:490–491. 1954.Google Scholar
15. Sell, H. M., Luecke, R. W., Taylor, B. M., and Hamner, C. L. Changes in chemical composition of the stems of red kidney bean plants treated with 2,4-dichlorophenoxyacetic acid. Plant Physiol. 24:295–299. 1949.CrossRefGoogle Scholar
16. Shaw, W. C., Willard, C. J., and Bernard, R. L. The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on wheat, oats, barley and the legumes under-seeded in these crops. Ohio Agr. Exp. Sta. Res. Bul. 761. 1955.Google Scholar
17. Skoog, F. Substances involved in normal growth and differentiation of plants. Brookhaven Symposia in Biology 6 (BNL 258): 1–21. 1954.Google Scholar
18. Stumpf, P. K. A colorimetric method of determination of desoxyribonucleic acid. Jour. Biol. Chem. 169:367–371. 1947.Google Scholar
19. Sunderland, N., Heyes, J. K., and Brown, R. Protein and respiration in the apical region of the shoot of Lupinus albus. Jour. Exp. Bot. 8:55–70. 1957.Google Scholar
20. Switzer, C. M. Effects of herbicides and related chemicals on oxidation and phosphorylation by isolated soybean mitochondria. Plant Physiol. 32:42–44. 1957.CrossRefGoogle ScholarPubMed
21. Tanada, T. Effect of ribonuclease on salt absorption by excised mung bean roots. Plant Physiol. 31:251–253. 1956.Google Scholar
22. Thimann, K. V., and Loos, G. M. Protein synthesis during water uptake by potato tissue. Plant Physiol. 32:274–279. 1957.Google Scholar
23. Umbreit, W. W., Burris, R. H., and Stauffer, J. F. Manometric Techniques (3rd Ed.) Burgess Publ. Co., Minneapolis. 1957.Google Scholar