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Aurora kinase A is essential for correct chromosome segregation in mouse zygote

Published online by Cambridge University Press:  15 July 2015

Veronika Kovarikova ,
Jan Burkus ,
Pavol Rehak ,
Adela Brzakova ,
Petr Solc  and
Vladimir Baran
Veronika Kovarikova
Affiliation:
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Jan Burkus
Affiliation:
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Pavol Rehak
Affiliation:
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Adela Brzakova
Affiliation:
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
Petr Solc
Affiliation:
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
Vladimir Baran*
Affiliation:
Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4, 040 01 Kosice, Slovakia.
*
All correspondence to: Vladimir Baran. Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4, 040 01 Kosice, Slovakia. E-mail: baran@saske.sk
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Summary

Aurora-A kinase (AURKA), a member of the serine/threonine protein kinase family, is involved in multiple steps of mitotic progression. It regulates centrosome maturation, mitotic spindle formation, and cytokinesis. While studied extensively in somatic cells, little information is known about AURKA in the early cleavage mouse embryo with respect to acentrosomal spindle assembly. In vitro experiments in which AURKA was inactivated with specific inhibitor MLN8237 during the early stages of embryogenesis documented gradual arrest in the cleavage ability of the mouse embryo. In the AURKA-inhibited 1-cell embryos, spindle formation and anaphase onset were delayed and chromosome segregation was defective. AURKA inhibition increased apoptosis during early embryonic development. In conclusion these data suggest that AURKA is essential for the correct chromosome segregation in the first mitosis as a prerequisite for normal later development after first cleavage.

Keywords

ApoptosisAurora AMLN8237Mouse zygoteSpindle
Type
Research Article
Information
Zygote , Volume 24 , Issue 3 , June 2016 , pp. 326 - 337
Copyright
Copyright © Cambridge University Press 2015 

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