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Combination of spindle and first polar body chromosome images for the enhanced prediction of developmental potency of mouse metaphase II oocytes

Published online by Cambridge University Press:  13 October 2016

Yukou Sugano ,
Manami Yazawa ,
Sachio Takino ,
Sueo Niimura  and
Hideaki Yamashiro
Yukou Sugano
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Manami Yazawa
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Sachio Takino
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Sueo Niimura
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Hideaki Yamashiro*
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
*
All correspondence to: Hideaki Yamashiro. Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan. Tel:/Fax: +81 25 262 6596. E-mail: hyamashiro@agr.niigata-u.ac.jp
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Summary

The objective of this study was to classify spindle and first polar body (PB1) chromosome images in ovulated mouse oocytes over time to predict the developmental competence of metaphase II (MII) oocytes. Oocytes were collected at 12, 15, 20, and 25 h after human chorionic gonadotropin (hCG) injection, and stained for spindle tubulin, chromosomes, and PB1 chromosomes. MII spindle morphology was classified as tapered type or barrel type and PB1 chromosomes were categorized as aggregated, separated, dot, or collapsed. To determine whether differences in spindle and PB1 images in MII oocytes are associated with fertilization success, we performed in vitro fertilization (IVF) at various times after hCG injection. Barrel-type spindles and aggregate-type PB1 were dominant at 12 h after hCG injection. Oocyte spindles collected 1 h after injection were tapered, and PB1 chromosomes were separated. At 20 and 25 h after treatment, spindle and PB1 images were classified as collapsed. The rate of development to 2-cell embryos after IVF did not differ between the 12 h and 15 h treatments; however, it was significantly lower for the 25 h treatment than for other treatments. The rates of development to blastocysts at 12, 15, 20, and 25 h after hCG injection were 61, 46, 42, and 9%, respectively. MII oocytes with barrel-type spindles and aggregate-type PB1 had high rates of fertilization and blastocyst development, and spindle and PB1 characteristics were correlated with the outcomes of IVF and embryo culture. These results suggested that images of spindles combined with those of PB1 chromosomes enable the prediction of oocytic and/or embryonic quality.

Keywords

Developmental rateFirst polar bodyIVFMetaphase IISpindle
Type
Research Article
Information
Zygote , Volume 24 , Issue 6 , December 2016 , pp. 900 - 908
Copyright
Copyright © Cambridge University Press 2016 

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