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Comparison of two culture media on morphokinetics and ploidy status of sibling embryos

Published online by Cambridge University Press:  09 December 2021

Michael Urich ,
Muhammet Rasit Ugur ,
Fang Li ,
F. Nicholas Shamma ,
Ahmad Hammoud ,
Hanh N. Cottrell  and
Sule Dogan Open the ORCID record for Sule Dogan [Opens in a new window]
Michael Urich
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Muhammet Rasit Ugur
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Fang Li
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
F. Nicholas Shamma
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Ahmad Hammoud
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Hanh N. Cottrell
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Sule Dogan*
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
*
Author for correspondence: Sule Dogan. IVF Michigan, 37000 Woodward Ave #350 Bloomfield Hills, Michigan48304, USA. E-mail: suledogann@gmail.com
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Summary

To investigate the effects of culture media with different lactate concentrations on early embryonic development, data collected from our patients undergoing preimplantation genetic testing (PGT) were assessed using the EmbryoScope™ time-lapse culturing system. After intracytoplasmic sperm injection (ICSI), sibling oocytes were cultured in the same EmbryoScope (Vitrolife) slides including two different commercially available media. The patients with fewer than five mature oocytes were not included in the analyses. All embryos were hatched on day 3, and trophectoderm biopsies (n = 212) were performed accordingly. PGT for aneuploidy (PGT-A) on biopsied materials was carried out using next generation sequencing. Morphokinetic parameters, fertilization, irregular division, degeneration, blastulation, euploidy, and pregnancy rates of embryos cultured in LifeGlobal Global Total medium (LGGT) and Continuous Single Culture-NX Complete medium (CSCM-NXC) were compared. There were no differences observed in time to pronuclear fade, or in time spent as 2-cell (cc2) and 3-cell (s2), to 4-cell, 5-cell, morula and blastocyst stages (P > 0.05). Embryos reached the 2-cell (t2) and 3-cell (t3) stages significantly faster in LGGT (P < 0.05), whereas embryos grown in CSCM-NXC with lower lactate reached starting blastulation significantly sooner (P = 0.026). However, there were no statistical differences observed in fertilization, blastulation, degeneration, irregular division euploidy, and pregnancy rates between the two groups (P > 0.05). Even though pregnancy and fertilization rates did not indicate statistical differences, results are significant to provide better insight on potential roles of lactate in embryo development. These finding will advance the fundamental knowledge of human embryo development and assisted reproductive technologies.

Keywords

EuploidyLow lactate mediaMorphokineticsPreimplantation genetic testingTime-lapse system
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 410 - 415
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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