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Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa

Published online by Cambridge University Press:  29 October 2012

Alexander V. Makarevich ,
Eliska Spalekova ,
Lucia Olexikova ,
Elena Kubovicova  and
Zdenka Hegedusova
Alexander V. Makarevich*
Affiliation:
Animal Production Research Centre Nitra, Hlohovecká 2, 951 41 Lužianky near Nitra, Slovak Republic.
Eliska Spalekova
Affiliation:
Slovak Agricultural University, 94901 Nitra, Slovak Republic.
Lucia Olexikova
Affiliation:
Animal Production Research Centre Nitra, 951 41 Lužianky near Nitra, Slovak Republic.
Elena Kubovicova
Affiliation:
Animal Production Research Centre Nitra, 951 41 Lužianky near Nitra, Slovak Republic.
Zdenka Hegedusova
Affiliation:
Research Institute for Cattle Breeding Ltd. Rapotin, Vyzkumniku 267, 788 13 Vikyrovice, Czech Republic.
*
All correspondence to: Alexander V. Makarevich, Animal Production Research Centre Nitra, Hlohovecká 2, 951 41 Lužianky near Nitra, Slovak Republic. e-mail: makarevic@cvzv.sk
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Summary

The aim of the study was to examine the effects of insulin-like growth factor I (IGF-I) on ram sperm traits after hypothermic storage. Sperm ejaculates from Lacaune rams were diluted in a Tris extender, pooled, divided into groups of IGF-I doses tested (0, 10, 100 or 200 ng.ml−1) and stored (0–5°C) for 96 h. IGF-I elevated whole sperm motility as measured by a Computer-assisted Sperm Analyser (CASA) system, by 24 h (10 ng.ml−1) and 48 h (200 ng.ml−1) of storage, and by progressive movement on each day of storage. After 72 h the sperm samples were analysed for plasma membrane integrity (peanut agglutinin–fluorescein isothiocyanate), membrane stability (annexin V–Fluos) and apoptosis (Yo-Pro®-1) using fluorescence microscopy. The addition of IGF-I (at 100 or 200 ng.ml−1) reduced the ratio of sperm with disrupted membranes and the ratio of annexin V-labelled sperm. The ratio of apoptotic sperm was reduced by IGF-I given at 10 or 100 ng.ml−1 compared with control. Sperm fertilizing ability, determined at 48 h by an in vitro fertilization (IVF) test on bovine oocytes, was increased by IGF-I given at 100 ng.ml−1 from 47.0 to 67.7%. In conclusion, IGF-I maintained ram sperm functions following cooling storage and its effects were reflected in sperm fertilizing ability in vitro.

Keywords

Fertilizing abilityIGF-IMotilitySpermViability
Type
Research Article
Information
Zygote , Volume 22 , Issue 3 , August 2014 , pp. 305 - 313
Copyright
Copyright © Cambridge University Press 2012 

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References

Anonymous. Statistix for Windows. 2001. Version 8.0. User's Manual. Analytical Software. P.O. Box. 12185, Tallahassee, FL 32317–2185, USA.Google Scholar
Anzar, M., He, L., Buhr, M.M., Kroetsch, T.G. & Pauls, K.P. (2002). Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility. Biol. Reprod. 66, 354–60.CrossRefGoogle ScholarPubMed
Champion, Z.J., Vickers, M.H., Gravance, C.G., Breier, N.H. & Casey, P.J. (2002). Growth hormone or insulin-like growth factor-I extends longevity of equine spermatozoa in vitro . Theriogenology 57, 1793–800.Google Scholar
Garcia-Alvarez, O., Maroto-Morales, A., Martinez-Pastor, F., Fernandez-Santos, M.R., Esteso, M.C., Perez-Guzman, M.D. & Soler, A.J. (2009). Heterologous in vitro fertilization is a good procedure to assess the fertility of thawed ram spermatozoa. Theriogenology 71, 643–50.Google Scholar
Henrick, D.M., Kouba, A.J., Lackey, B.R., Boone, W.R. & Gray, S.L. (1998). Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biol. Reprod. 59, 330–37.CrossRefGoogle Scholar
Januskauskas, A., Johannisson, A. & Rodriguez-Martinez, H. (2003). Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology 60, 743–58.Google Scholar
Jones, J.I. & Clemmons, D.R. (1995). Insulin-like growth factors and their binding proteins: biological actions. Endocr. Rev. 16, 3–34.Google Scholar
Kurz, A., Viertel, D., Herrmann, A. & Muller, K. (2005). Localization of phosphatidylserine in boar sperm cell membranes during capacitation and acrosome reaction. Reproduction 130, 615–26.CrossRefGoogle ScholarPubMed
Lackey, B.R., Boone, W.R., Gray, S.L.L. & Henrick, D.M. (1998). Computer-assisted sperm motion analysis of bovine sperm treated with insulin-like growth factor I and II: implications as motility regulators and chemokinetic factors. System Biol. Reprod. Med. 41, 115–25.Google Scholar
Macpherson, M.L., Simmen, R.C.M., Simmen, F.A., Hernandez, J., Sheerin, B.R., Varner, D.D., Loomis, P., Cadario, M.E., Miller, C.D., Brinsko, S.P., Rigby, S. & Blanchard, T.L. (2002). Insulin-like growth factor-I and insulin-like growth factor binding protein-2 and -5 in equine seminal plasma: association with sperm characteristics and fertility. Biol. Reprod. 67, 648–54.Google Scholar
Makarevich, A.V. & Markkula, M. (2002). Apoptosis and proliferation potential of bovine embryos stimulated with insulin-like growth factor-I during in vitro maturation and culture. Biol. Reprod. 66, 386–92.Google Scholar
Marchetti, C., Obert, G., Deffosez, A., Formstecher, P. & Marchetti, P. (2002). Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm. Hum. Reprod. 17, 1257–65.CrossRefGoogle ScholarPubMed
Martin, G., Sabido, O., Durand, P. & Levy, R. (2004). Cryopreservation induces an apoptosis-like mechanism in bull sperm. Biol. Reprod. 71, 28–37.CrossRefGoogle ScholarPubMed
Maxwell, W.M. & Salamon, S. (1993). Liquid storage of ram semen: a review. Reprod. Fert. Dev. 6, 613–38.Google Scholar
Miah, A.G., Salma, U., Takagi, Y., Kohsaka, T., Hamano, K.-I. & Tsujii, H. (2008). Effect of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa. Reprod. Med. Biol. 7, 29–36.Google Scholar
Minelli, A., Liguori, L., Collodel, G., Lattaioli, P. & Castellini, C. (2001a). Effects of the purified IGF-I complex on the capacitation and acrosome reaction of rabbit spermatozoa. J. Exp. Zool. 290, 311–7.Google Scholar
Minelli, A., Moroni, M. & Castellini, C. (2001b). Isolation and purification of the IGF-I protein complex from rabbit seminal plasma: effects on sperm motility and viability. J. Exp. Zool. 290, 279–90.CrossRefGoogle ScholarPubMed
Naz, R,K, & Minhas, B.S. (1995). Enhancement of sperm function for treatment of male infertility. J. Androl. 16, 384–8.CrossRefGoogle ScholarPubMed
Naz, R.K. & Padman, P. (1999). Identification of insulin-like growth factor (IGF)-1 receptor in human sperm cell. Arch. Androl. 43, 153–9.Google Scholar
Park, E.K., Chu, J.P., Parkinson, T.J., Cockrem, J.F., Han, K.S., Blair, H.T., Kim, T.Y., Yoon, J.T. & Lee, Y.S. (2010). Rams genetically superior for IGF-I do not exhibit improved male reproductive traits. Anim. Reprod. Sci. 118, 223–30.Google Scholar
Peňa, F. J., Johannisson, A., Wallgren, M. & Rodriguez-Martinez, H. (2003). Assessment of fresh and frozen-thawed boar semen using an annexin-V assay: a new method of evaluating sperm membrane integrity. Theriogenology 60, 677–89.CrossRefGoogle ScholarPubMed
Peňa, F.J., Saravia, F., Johannisson, A., Walgren, M. & Rodrigues-Martinez, H. (2005). A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa. Int. J. Androl. 28, 107–14.Google Scholar
Salamon, S. & Maxwell, W.M.C. (2000). Storage of ram semen. Anim. Reprod. Sci. 62, 77–111.Google Scholar
Sanchez-Luengo, S., Fernandez, P.J. & Romeu, A. (2005). Insulin growth factors may be implicated in human sperm capacitation. Fertil. Steril. 83, 1064–6.Google Scholar
Sauerwein, H., Breier, B.H., Gallaher, B.W., Gotz, C., Kufner, G., Montag, T., Vickers, M.H. & Schallenberger, E. (2000). Growth hormone treatment of breeding bulls used for artificial insemination improves fertilization rates. Domest. Anim. Endocrinol. 18, 145–58.Google Scholar
Selvaraju, S., Reddy, I.J., Nandi, S., Rao, S.B.N. & Ravindra, J.P. (2009). Influence of IGF-I on buffalo (Bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro . Anim. Reprod. Sci. 113, 60–70.Google Scholar
Selvaraju, S., Nandi, S., Siva Subramani, T., Raghavendra, B.S., Rao, S.B.N. & Ravindra, J.P. (2010). Improvement in buffalo (Bubalus bubalis) spermatozoa functional parameters and fertility in vitro: effect of insulin-like growth factor-I. Theriogenology. 73, 1–10.Google Scholar
Trzcinska, M., Bryla, M. & Smorag, Z. (2008). Effect of liquid storage on membrane integrity and mitochondrial activity: a new diagnostic method of evaluating boar sperm quality. J. Anim. Feed Sci. 17, 372–80.Google Scholar
Vickers, M.H., Casey, P.J., Champion, Z.J., Gravance, C.G. & Breier, B.H. (1999). IGF-I treatment increases motility and improves morphology of immature spermatozoa in the GH-deficient dwarf (dw /dw) rat. Growth Horm. & IGF-I Res. 9, 236–40.Google Scholar
Waterhouse, K.E., De Angelis, P.M., Haugan, T., Paulenz, H., Hofmo, P.O. & Farstad, W. (2004). Effect of in vitro storage time and semen-extender on membrane quality of boar sperm assessed by flow cytometry. Theriogenology 62, 1638–51.Google Scholar