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Exposure to follicular fluid during oocyte maturation and oviductal fluid during post-maturation does not improve in vitro embryo production in the horse

Published online by Cambridge University Press:  20 September 2017

Cécile Douet ,
Olivia Parodi ,
Nicola Antonio Martino ,
Giovanni Michele Lacalandra ,
Michele Nicassio ,
Fabrice Reigner ,
Stefan Deleuze ,
Maria Elena Dell'Aquila  and
Ghylène Goudet
Cécile Douet
Affiliation:
PRC, INRA, CNRS, IFCE, Université de Tours, 37380 Nouzilly, France.
Olivia Parodi
Affiliation:
PRC, INRA, CNRS, IFCE, Université de Tours, 37380 Nouzilly, France.
Nicola Antonio Martino
Affiliation:
Istituto Zooprofilattico Sperimentale per la Puglia e la Basilicata, Foggia, Italy. Università degli Studi di Bari Aldo Moro, Dipartimento di Bioscienze, Biotecnologie e Biofarmaceutica, 70010, Polo di Valenzano, Bari, Italy.
Giovanni Michele Lacalandra
Affiliation:
Università degli Studi di Bari Aldo Moro, Dipartimento dell'Emergenza e Trapianti d'Organo (DETO), 70010 Polo di Valenzano, BariItaly.
Michele Nicassio
Affiliation:
Università degli Studi di Bari Aldo Moro, Dipartimento dell'Emergenza e Trapianti d'Organo (DETO), 70010 Polo di Valenzano, BariItaly.
Fabrice Reigner
Affiliation:
PAO, INRA, 37380, Nouzilly, France.
Stefan Deleuze
Affiliation:
Faculté de Médecine vétérinaire, Département des Sciences Cliniques-Clinique Equine, Université de Liège, B-4000 Liège, Belgium.
Maria Elena Dell'Aquila
Affiliation:
Università degli Studi di Bari Aldo Moro, Dipartimento di Bioscienze, Biotecnologie e Biofarmaceutica, 70010, Polo di Valenzano, Bari, Italy.
Ghylène Goudet*
Affiliation:
INRA, UMR 85, Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
*
All correspondence to: Ghylène Goudet, INRA, UMR 85, Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France. Tel:+33 2 47 42 79 41. Fax: +33 2 47 42 77 43. E-mail: ghylene.goudet@inra.fr
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Summary

Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low. No conventional in vitro fertilization (IVF) technique for equine embryo production is available. The development of culture conditions able to mimic the maturation of the oocyte in preovulatory follicular fluid (pFF) and the post-maturation in oviductal fluid (OF) may improve embryo production in vitro. Our aim was to analyse the effect of in vitro maturation in pFF and incubation in OF on in vitro maturation of equine oocytes, fertilization using conventional IVF or ICSI, and embryo development after culture in synthetic oviductal fluid (SOF) or DMEM-F12. Oocytes collected from slaughtered mares or by ovum pick up were matured in vitro in pFF or semi-synthetic maturation medium (MM). The in vitro maturation, fertilization and development rates were not statistically different between pFF and MM. After in vitro maturation, oocytes were incubated with or without OF. Post-maturation in OF did not significantly improve the fertilization and development rates. Thus, in our study, exposure to physiological fluids for oocyte maturation and post-maturation does not improve in vitro embryo production in the horse.

Keywords

EquineIVMIVFOocyteSpermatozoa
Type
Research Article
Information
Zygote , Volume 25 , Issue 5 , October 2017 , pp. 612 - 630
Copyright
Copyright © Cambridge University Press 2017 

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