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Expression of apoptotic genes in immature and in vitro matured equine oocytes and cumulus cells

Published online by Cambridge University Press:  21 September 2011

P.M.M. Leon ,
V.F. Campos ,
C. Kaefer ,
K.R. Begnini ,
A.J.A. McBride ,
O.A. Dellagostin ,
F.K Seixas ,
J.C. Deschamps  and
T. Collares
P.M.M. Leon
Affiliation:
Laboratório de Embriologia Molecular e Transgênese, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
V.F. Campos
Affiliation:
Laboratório de Embriologia Molecular e Transgênese, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
C. Kaefer
Affiliation:
Laboratório de Embriologia Molecular e Transgênese, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
K.R. Begnini
Affiliation:
Laboratório de Embriologia Molecular e Transgênese, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
A.J.A. McBride
Affiliation:
Laboratório de Biologia Molecular, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
O.A. Dellagostin
Affiliation:
Laboratório de Biologia Molecular, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
F.K Seixas
Affiliation:
Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
J.C. Deschamps
Affiliation:
Laboratório de Embriologia Molecular e Transgênese, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
T. Collares*
Affiliation:
Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, RS, Brazil.
*
All correspondence to: Tiago Collares. Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, RS, Brazil. Tel: +55 53 3275 7588. e-mail: collares.t@gmail.com
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Summary

The gene expression of Bax, Bcl-2, survivin and p53, following in vitro maturation of equine oocytes, was compared in morphologically distinct oocytes and cumulus cells. Cumulus–oocyte complexes (COC) were harvested and divided into two groups: G1 – morphologically healthy cells; and G2 – less viable cells or cells with some degree of atresia. Total RNA was isolated from both immature and in vitro matured COC and real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to quantify gene expression. Our results showed there was significantly higher expression of survivin (P < 0.05) and lower expression of p53 (P < 0.01) in oocytes compared with cumulus cells in G1. No significant difference in gene expression was observed following in vitro maturation or in COC derived from G1 and G2. However, expression of the Bax gene was significantly higher in cumulus cells from G1 (P < 0.02).

Keywords

ApoptosisGene expressionIn vitro maturationqRT-PCR
Type
Research Article
Information
Zygote , Volume 21 , Issue 3 , August 2013 , pp. 279 - 285
Copyright
Copyright © Cambridge University Press 2011 

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