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Histone hyperacetylation and DNA methylation interplay during murine spermatogenesis

Published online by Cambridge University Press:  15 August 2019

Liliana Burlibaşa Open the ORCID record for Liliana Burlibaşa [Opens in a new window] ,
Andreea Carmen Ionescu  and
Delia-Mihaela Dragusanu
Liliana Burlibaşa*
Affiliation:
University of Bucharest, Faculty of Biology, Genetics Department, Bucharest, Romania
Andreea Carmen Ionescu
Affiliation:
University of Bucharest, Faculty of Biology, Genetics Department, Bucharest, Romania
Delia-Mihaela Dragusanu
Affiliation:
University of Bucharest, Faculty of Biology, Genetics Department, Bucharest, Romania
*
Address for correspondence: Liliana Burlibaşa. Nos. 1–3 Aleea Portocalilor, Bucharest, Romania. Tel:/Fax: +40 213181565. E-mails: liliana_burlibasa@yahoo.com.au; liliana.burlibasa@bio.unibuc.ro
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Summary

Male germ cell development is a critical period during which epigenetic patterns are established and maintained. The progression from diploid spermatogonia to haploid spermatozoa involves the incorporation of testis-specific histone variants, mitotic and meiotic divisions, haploid gene expression, histone–protamine transitions and massive epigenetic reprogramming. Understanding the protein players and the epigenetic mark network involved in the setting of the epigenetic programme in spermatogenesis is an exciting new clue in the field of reproductive biology with translational outcomes. As information in the existing literature regarding cross-talk between DNA methylation and histone hyperacetylation in the advanced stages of murine spermatogenesis is still scarce and controversial we have investigated the effect of a DNA-methyltransferase inhibitor, 5-aza-2′-deoxycytidine, at the cytological and molecular level (by transmission electron microscopy, immunocytochemistry and immunoprecipitation methods). Our results revealed an important role for regulation of DNA methylation in controlling histone hyperacetylation and chromatin remodelling during spermatogenesis.

Keywords

5-Aza-2′-deoxycytidineDNA methylationEpigenetic marksH4 hyperacetylationSpermatogenesis
Type
Research Article
Information
Zygote , Volume 27 , Issue 5 , October 2019 , pp. 305 - 314
Copyright
© Cambridge University Press 2019 

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Footnotes

*

These authors made the same contribution to this work.

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