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Influence of hyaluronan accumulation during cumulus expansion on in vitro porcine oocyte maturation

Published online by Cambridge University Press:  01 November 2008

Masaki Yokoo ,
Naoko Kimura ,
Hiroyuki Abe  and
Eimei Sato
Masaki Yokoo*
Affiliation:
Innovation of New Biomedical Engineering Centre, Tohoku University, 1–1 Seiryou-machi, Aoba-ku, Sendai 980–8575, Japan. Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981–8555, Japan. Division of Biofunctional Science, Tohoku University Biomedical Engineering Research Organization (TUBERO), 2–1 Seiryou-machi, Aoba-ku, Sendai 980–8575, Japan.
Naoko Kimura
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981–8555, Japan.
Hiroyuki Abe
Affiliation:
Division of Biofunctional Science, Tohoku University Biomedical Engineering Research Organization (TUBERO), 2–1 Seiryou-machi, Aoba-ku, Sendai 980–8575, Japan.
Eimei Sato
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981–8555, Japan.
*
All correspondence to: Masaki Yokoo. Innovation of New Biomedical Engineering Centre, Tohoku University, 1–1 Seiryou-machi, Aoba-ku, Sendai 980–8575, Japan. Tel: +81 22 717 7122. Fax: +81 22 717 7104. e-mail: myokoo@m.tains.tohoku.ac.jp
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Summary

During oocyte maturation, the cumulus–oocyte complexes (COCs) expand dramatically. This phenomenon, which is known as cumulus expansion, is the result of the synthesis and accumulation of hyaluronan in the extracellular space between cumulus cells. The purpose of this study was to investigate the effect of 6-diazo-5-oxo-l-norleucine (DON), an inhibitor of hyaluronan synthesis, on cumulus expansion during in vitro porcine oocyte maturation and hyaluronan accumulation within COCs. Further, this study aimed to examine the influence of hyaluronan accumulation within COCs on the rate of oocyte maturation. Cumulus expansion was observed during in vitro maturation. However, the addition of DON to the maturation medium significantly inhibited cumulus expansion. The total inhibition of hyaluronan accumulation within COCs was observed with the use of confocal microscopy. Moreover, a positive correlation between the area of cumulus expansion and the rate of oocyte maturation was observed. These results demonstrate that the hyaluronan accumulation within the COCs during oocyte maturation affects oocyte maturation. On the basis of these results, we propose that hyaluronan accumulation within the COCs during cumulus expansion is a necessary step in the porcine oocyte maturation process.

Keywords

Cumulus expansionHyaluronanOocyteOocyte maturationPig
Type
Research Article
Information
Zygote , Volume 16 , Issue 4 , November 2008 , pp. 309 - 314
Copyright
Copyright © Cambridge University Press 2008

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