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Effects of bone morphogenetic protein 4 (BMP4) on in vitro development and survival of bovine preantral follicles enclosed in fragments ovarian tissue

Published online by Cambridge University Press:  16 March 2017

Ellen de Vasconcelos da Cunha ,
Glaucinete Borges de Souza ,
José Renato de Sousa Passos ,
Anderson Weiny Barbalho Silva ,
Andressa Minussi Dau ,
Márcia Viviane Alves Saraiva ,
Raimundo Nonato Braga Lobo  and
José Roberto Viana Silva
Ellen de Vasconcelos da Cunha
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil.
Glaucinete Borges de Souza
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil.
José Renato de Sousa Passos
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil.
Anderson Weiny Barbalho Silva
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil.
Andressa Minussi Dau
Affiliation:
Laboratory of Biotechnology and Animal Reproduction (BioRep), Federal University of Santa Maria (UFSM) Santa Maria, RS, Brazil.
Márcia Viviane Alves Saraiva
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil.
Raimundo Nonato Braga Lobo
Affiliation:
Brazilian Agricultural Research Corporation (EMBRAPA) Goats and Sheep, Sobral, CE, Brazil.
José Roberto Viana Silva*
Affiliation:
Biotechnology Nucleus of Sobral − NUBIS, Federal University of Ceara, Av. Comandante Maurocélio Rocha Ponte 100, CEP 62041–040, Sobral, CE, Brazil.
*
All correspondence to José Roberto Viana Silva. Biotechnology Nucleus of Sobral − NUBIS, Federal University of Ceara, Av. Comandante Maurocélio Rocha Ponte 100, CEP 62041–040, Sobral, CE, Brazil. Tel:/Fax: +55 88 36118000. E-mail: jrvsilva@ufc.br
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Summary

The aim of this study was to evaluate the effects of different concentrations of BMP4 on activation, development and mRNA expression of GDF9, BMP15, PCNA, Bax and Bcl2 in cultured bovine follicles enclosed in ovarian tissues. Ovarian tissue fragments were cultured for 6 days in α-MEM+ alone or supplemented with different concentrations of BMP4 (10, 50 or 100 ng/ml). Classical histology was performed to analyze follicle growth and morphology, while real-time PCR was used to analyze mRNA levels in fresh and cultured tissues. After 6 days, the culture of ovarian tissue in α-MEM+ alone or supplemented with 10, 50 or 100 ng/ml BMP4 promoted follicular activation. The different concentrations of BMP4 maintained the percentage of normal follicles similar to results of the control. The presence of 100 ng/ml BMP-4 in culture medium increased oocyte and follicular diameters of primary and secondary follicles when compared with those follicles from uncultured control or cultured in α-MEM+ alone (P < 0.05). The tissues cultured in the presence of increasing concentrations of BMP4 had an increase in mRNA expression of the tested genes, but despite this the differences were not statistically significant. In conclusion, 100 ng/ml BMP4 promotes an increase in diameters of follicles and oocytes of primary and secondary follicles after 6 days of in vitro culture.

Keywords

ActivationCowCultureOvaryPrimordial follicle
Type
Research Article
Information
Zygote , Volume 25 , Issue 3 , June 2017 , pp. 256 - 264
Copyright
Copyright © Cambridge University Press 2017 

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