Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-21T12:21:44.288Z Has data issue: false hasContentIssue false
  • English
  • Français

Supplementation with the histone deacetylase inhibitor trichostatin A during in vitro culture of bovine embryos

Published online by Cambridge University Press:  26 August 2011

Clara Slade Oliveira ,
Naiara Zoccal Saraiva ,
Marcela Maria de Souza ,
Tatiane de Almeida Drummond Tetzner ,
Marina Ragagnin de Lima  and
Joaquim Mansano Garcia
Clara Slade Oliveira*
Affiliation:
UNESP–Via de Acesso Professor Paulo Donato Castellane, CEP 14884–900, Jaboticabal, São Paulo, Brazil.
Naiara Zoccal Saraiva
Affiliation:
Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, Access Road Professor Paulo Donato Castellane, zip code 14884–900, Jaboticabal, Brazil.
Marcela Maria de Souza
Affiliation:
Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, Access Road Professor Paulo Donato Castellane, zip code 14884–900, Jaboticabal, Brazil.
Tatiane de Almeida Drummond Tetzner
Affiliation:
Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, Access Road Professor Paulo Donato Castellane, zip code 14884–900, Jaboticabal, Brazil.
Marina Ragagnin de Lima
Affiliation:
Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, Access Road Professor Paulo Donato Castellane, zip code 14884–900, Jaboticabal, Brazil.
Joaquim Mansano Garcia
Affiliation:
Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, Access Road Professor Paulo Donato Castellane, zip code 14884–900, Jaboticabal, Brazil.
*
All correspondence to: Clara Slade Oliveira. UNESP–Via de Acesso Professor Paulo Donato Castellane, CEP 14884–900, Jaboticabal, São Paulo, Brazil. Tel: +55 16 32092633/+55 16 97242262. Fax: +55 21 25258804. E-mail: claraslade@gmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

Trichostatin A (TSA) is a histone deacetylase inhibitor that induces histone hyperacetylation and increases gene expression levels. The aim of the present study was to establish a suitable condition for the use of TSA in in vitro cultures of bovine embryos, and to determine whether TSA would increase blastocyst rates by improvement of chromatin remodelling during embryonic genome activation and by increasing the expression of crucial genes during early development. To test this hypothesis, 8-cell embryos were exposed to four concentrations of TSA for different periods of time to establish adequate protocols. In a second experiment, three experimental groups were selected for the evaluation of embryo quality based on the following parameters: apoptosis, total cell number and blastocyst hatching. TSA promoted embryonic arrest and degeneration at concentrations of 15, 25 and 50 nM. All treated groups presented lower blastocyst rates. Exposure of embryos to 5 nM for 144 h and to 15 nM for 48 h decreased blastocyst hatching. However, the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL) assay revealed similar apoptosis rates and total cell numbers in all groups studied. Although, in the present study, TSA treatment did not improve the parameters studied, the results provided background information on TSA supplementation during in vitro culture of bovine embryos and showed that embryo quality was apparently not affected, despite a decrease in blastocyst rate after exposure to TSA.

Keywords

CattleEmbryonic developmentHistone deacetylase inhibitionHistone hyperacetylationTrichostatin A
Type
Research Article
Information
Zygote , Volume 21 , Issue 1 , February 2013 , pp. 59 - 63
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aoki, F., Worrad, D.M. & Schultz, R.M. (1997). Regulation of transcriptional activity during the first and second cell cycles in the preimplantation mouse embryo. Dev. Biol. 181, 296–307.CrossRefGoogle ScholarPubMed
Avery, B., Jorgensen, C.B., Madison, V. & Greve, T. (1992). Morphological development and sex of bovine in vitro-fertilized embryos. Mol. Reprod. Dev. 32, 265–70.CrossRefGoogle ScholarPubMed
Betts, D.H. & King, W.A. (2001). Genetic regulation of embryo death and senescence. Theriogenology 55, 171–91.CrossRefGoogle ScholarPubMed
Brackett, B.G., Bousquet, D., Boice, M.L., Donawick, W.J., Evans, J.F. & Dressel, M.A. (1982). Normal development following in vitro fertilization in the cow. Biol. Reprod. 27, 147–58.CrossRefGoogle ScholarPubMed
Dean, W., Santos, F., Stojkovic, M., Zakhartchenko, V., Walter, J., Wolf, E. & Reik, W. (2001). Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos. Proc. Natl. Acad. Sci. USA 98, 13734–38.CrossRefGoogle ScholarPubMed
Ding, X., Wang, Y., Zhang, D., Wang, Y., Guo, Z. & Zhang, Y. (2008). Increased pre-implantation development of cloned bovine embryos treated with 5-aza-2’-deoxycytidine and trichostatin A. Theriogenology 70, 622–30.CrossRefGoogle ScholarPubMed
Enright, B.P., Kubota, C., Yang, X. & Tian, X.C. (2003). Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin A or 5-aza-2′-deoxycytidine. Biol. Reprod. 69, 896–901.CrossRefGoogle ScholarPubMed
Iager, A.E., Ragina, N.P., Ross, P.J., Beyhan, Z., Cunniff, K., Rodriguez, R.M. & Cibelli, J.B. (2008). Trichostatin A improves histone acetylation in bovine somatic cell nuclear transfer early embryos. Cloning Stem Cells 10, 371–79.CrossRefGoogle ScholarPubMed
Ikeda, S., Tatemizo, A., Iwamoto, D., Taniguchi, S. & Hoshino, Y. (2009). Enhancement of histone acetylation by trichostatin A during in vitro fertilization of bovine oocytes affects cell number of the inner cell mass of the resulting blastocysts. Zygote 17, 209–15.CrossRefGoogle ScholarPubMed
Johnstone, R.W. (2002). Histone-deacetylase inhibitors: novel drugs for the treatment of cancer. Nat. Rev. Drug. Discov. 1, 287–99.CrossRefGoogle ScholarPubMed
Lequarre, A.S., Marchandise, J., Moreau, B., Massip, A. & Donnay, I. (2003). Cell cycle duration at the time of maternal zygotic transition for in vitro produced bovine embryos: effect of oxygen tension and transcription inhibition. Biol. Reprod. 69, 1707–13.CrossRefGoogle ScholarPubMed
Maalouf, W.E., Alberio, R. & Campbell, K.H.S. (2008). Differential acetylation of histone H4 lysine during development of in vitro fertilized, cloned and parthenogenetically activated bovine embryos. Epigenetics 3, 199–209.CrossRefGoogle ScholarPubMed
Ma, P. & Schultz, R.M. (2008). Histone deacetylase 1 (HDAC1) regulates histone acetylation, development, and gene expression in preimplantation mouse embryos. Dev. Biol. 319, 110–20.CrossRefGoogle ScholarPubMed
Meirelles, F.V., Caetano, A.R., Watanabe, Y.F., Ripamonte, P., Carambula, S.F., Merighe, G.K. & Garcia, S.M. (2004). Genome activation and developmental block in bovine embryos. Ann. Reprod. Sci. 82–83, 13–20.CrossRefGoogle ScholarPubMed
Oliveira, C.S., Saraiva, N.Z., Souza, M.M., Tetzner, T.A.D., Lima, M.R. & Garcia, J.M. (2010). Effects of histone hyperacetylation on the preimplantation development of male and female bovine embryos. Reprod. Fertil. Dev. 22, 1041–8.CrossRefGoogle ScholarPubMed
Paula-Lopes, F.F. & Hansen, P.J. (2002). Apoptosis is an adaptive response in bovine preimplantation embryos that facilitates survival after heat shock. Biochem. Biophys. Res. Commun. 295, 37–42.CrossRefGoogle ScholarPubMed
Schubeler, D., Macalpine, D.M., Scalzo, D., Wirbelauer, C., Kooperberg, C., Van Leeuwen, F., Gottschling, D.E., O'Neil, L.P., Turner, B.M., Delrow, J., Bell, S.P. & Groudine, M. (2004). The histone modification pattern of genes revealed through genome-wide chromatin analysis of a higher eukaryote. Genes Dev. 18, 1263–71.CrossRefGoogle ScholarPubMed
Wee, G., Shim, J.J., Koo, D.B., Chae, J.I. & Lee, K.K. (2007). Epigenetic alteration of the donor cells does not recapitulate the reprogramming of DNA methylation in cloned embryos. Reproduction 134, 781–87.CrossRefGoogle Scholar
Xu, K.P., Yadav, B.R., King, W.A. & Betteridge, K.J. (1992). Sex-related differences in developmental rates of bovine embryos produced and cultured in vitro. Mol. Reprod. Dev. 31, 249–52.CrossRefGoogle ScholarPubMed