Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-21T17:18:13.784Z Has data issue: false hasContentIssue false
  • English
  • Français

Prognostic factors in carcinoma of the larynx: relevance of DNA ploidy, S-fractions and localization of the tumour

Published online by Cambridge University Press:  29 June 2007

Davorin Đanić ,
Damir Miličić ,
Drago Prgomet  and
Dinko Leović
Davorin Đanić*
Affiliation:
Department of Otolaryngology, General Hospital ‘Dr. Josip Benčević, 35 000 Slavonski Brod, Croatia.
Damir Miličić
Affiliation:
Department of Otolaryngology, General Hospital ‘Dr. Josip Benčević, 35 000 Slavonski Brod, Croatia.
Drago Prgomet
Affiliation:
Department of Otolaryngology, General Hospital ‘Dr. Josip Benčević, 35 000 Slavonski Brod, Croatia.
Dinko Leović
Affiliation:
Department of Otolaryngology, General Hospital ‘Dr. Josip Benčević, 35 000 Slavonski Brod, Croatia.
*
Address for correspondence: Davorin Đanić, ENT Department, General Hospital ‘Dr Josip Benčević’, 35 000 Slavonski Brod, Croatia. Fax: 00385-35-446121 e-mail: opbolsb@alf.tel.hr
Get access Rights & Permissions [Opens in a new window]

Abstract

The influence of the cell cycle profile and the site of the primary tumour on the overall survival were examined in 36 patients with squamous cell carcinoma of the larynx. DNA ploidy (p = 0.0091), the overall proliferative activity (p = 0.0001), the overall proliferative activity of diploid tumour cells (p = 0.0017) and primary tumour site (p = 0.0008) were found to be significant single prognostic factors of the overall survival. Multivariate analysis showed that only the overall proliferative activity was prognostically significant (p = 0.013). The results of the study show that the supraglottic site of the tumours correlates significantly with DNA ploidy (p = 0.0334) and the overall proliferative activity of tumour cells (p = 0.0159), whereas the correlation with proliferative activity of diploid tumour cells (p = 0.1416) has not been confirmed by this study. Glottic tumours showed a prognostically significant correlation with the overall proliferative activity (p = 0.0037) and proliferative activity of diploid tumour (p = 0.0054). Such a prognostic correlation was not found for DNA ploidy (p = 0.6542).

Keywords

LarynxCarcinoma, squamous cellDNA, neoplasmPrognosis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 113 , Issue 6 , June 1999 , pp. 538 - 541
Copyright
Copyright © JLO (1984) Limited 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akervall, J., Jin, Y., Baldetrop, B., Mertens, F., Wennerberg, J. (1998) Complex karyotypes in flow cytometrically DNA diploid squamous carcinomas of the head and neck. British Journal of Cancer 77(7): 1082–1088.CrossRefGoogle Scholar
Barona-de-Guzman, R., Martorell, M. A., Basterra, J., Armengot, M., Montoro, A., Montoro, J. (1993) Analysis of DNA content in supraglottic epidermoid carcinoma. Otolaryngology – Head and Neck Surgery 108(6): 706–710.CrossRefGoogle Scholar
Cooke, L., Cooke, T., Forster, G., Helliwell, T., Steel, P. (1991) Cellular DNA content and prognosis in surgically treated squamous carcinoma of the larynx. British Journal of Cancer 63: 1018–1020.CrossRefGoogle Scholar
Del Valle-Zapico, A., Fernandez, F. F., Suarez, A. R., Angulo, C. M., Quintela, J. R. (1998) Prognostic value of histopathologic parameters and DNA flow-cytometry in squamous cell carcinoma of the pyriform sinus. Laryngoscope 108: 269–272.CrossRefGoogle Scholar
El-Naggar, A. K., Lopez-Varela, V., Luna, M. A., Weber, R., Batsakis, J. G. (1992) Intratumoral DNA content heterogeneity in laryngeal squamous cell carcinoma. Archives of Otolaryngology – Head and Neck Surgery 118: 169–173.Google Scholar
Hedley, D., Friedlander, M., Taylor, I., Rugg, C., Musgrove, C. (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. Journal of Histochemistry and Cytochemistry 31: 1333–1335.CrossRefGoogle Scholar
Milroy, C. M., Devaney, K. O., Ferlito, A., Rinaldo, A. (1997) Role of DNA measurements of head and neck tumours. Annals of Otology, Rhinology and Laryngology 106: 801–804.CrossRefGoogle Scholar
Resnik, M. J. M., Uhlman, D., Niehaus, G. A., Gapany, M., Adams, G., Knapp, D., Jaszacs, W. (1995) Cervical lymph node status and survival in laryngeal carcinoma: prognostic factors. Annals of Otology, Rhinology and Laryngology 104: 685–694.Google Scholar
Sakr, W., Hussan, M., Zarbo, R., Enesly, J., Crissman, J. (1989) DNA quantitation and histologic characteristic of squamous cell carcinoma of the upper aerodigestive tract. Archives of Pathology and Laboratory Medicine 113: 1009–1014.Google Scholar
Snow, G. B., Patel, P., Leemans, C. R., Tiwari, R. (1992) Management of cervical lymph nodes in patients with head and neck cancer. European Archives of Otorhinolaryngology 249: 187–194.CrossRefGoogle Scholar
Tennvall, J., Wennerberg, J., Willem, R., Ask, A., Baldetorp, B., Ferno, M. (1993) DNA S-phase as a predictor of the outcome after radiotherapy. Acta Otolaryngologica (Stockholm) 113: 220–224.CrossRefGoogle ScholarPubMed
Toffoli, G., Franchin, G., Barzan, L., Cernigoi, C., Carbone, A., Sulfaro, S., Franceschi, S., Biocchi, M. (1995) Brief report: Prognostic importance of cellular DNA content in T1–2 No laryngeal squamous cell carcinoma treated with radiotherapy. Laryngoscope 105: 649–652.Google Scholar
Užarević, B., Batinić, D., Petrovečki, M., Marušić, M. (1992) Leukocyte membrane markers on cell populations defined by six flow cytometric bitmaps. Journal of Clinical Laboratory Analysis 6: 17–23.CrossRefGoogle Scholar
Vindelov, L.L., Christensen, I. J., Nissen, N. I. (1983) A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 3: 323–327.CrossRefGoogle Scholar
Welkoborsky, H. J., Dienes, H. P., Hini, M., Mann, W. J. (1995) Predicting recurrence and survival in patients with laryngeal cancer by means of DNA cytometry, tumor front grading and proliferation markers. Annals of Otology, Rhinology and Laryngology 104: 503–510.Google Scholar
Westerbeek, H. A., Mooi, W. J., Begg, C., Dessing, M., Balm, A. J. M. (1992) DNA flow cytometry of the head and neck carcinoma: the importance of uniform tissue sampling and tumour sites. European Archives of Otorhinolaryngology 249: 140–143.CrossRefGoogle Scholar
Wolf, G. T., Fisher, S. G., Truelsom, J. M., Beals, T. F. (1994) DNA content and regional metastases in patients with advanced laryngeal squamous cell carcinoma. Laryngoscope 104: 479–483.CrossRefGoogle Scholar