LiDAR coverage of a large contiguous area within the Mirador-Calakmul Karst Basin (MCKB) of northern Guatemala has identified a concentration of Preclassic Maya sites (ca. 1000 b.c.–a.d. 150) connected by causeways, forming a web of implied social, political, and economic interactions. This article is an introduction to one of the largest, contiguous, regional LiDAR studies published to date in the Maya Lowlands. More than 775 ancient Maya settlements are identified within the MCKB, and 189 more in the surrounding karstic ridge, which we condensed into 417 ancient cities, towns, and villages of at least six preliminary tiers based on surface area, volumetrics, and architectural configurations. Many tiered sites date to the Middle and Late Preclassic periods, as determined by archaeological testing, and volumetrics of contemporaneously constructed and/or occupied architecture with similar morphological characteristics. Monumental architecture, consistent architectural formats, specific site boundaries, water management/collection facilities, and 177 km of elevated Preclassic causeways suggest labor investments that defy organizational capabilities of lesser polities and potentially portray the strategies of governance in the Preclassic period. Settlement distributions, architectural continuities, chronological contemporaneity, and volumetric considerations of sites provide evidence for early centralized administrative and socio-economic strategies within a defined geographical region.