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Monitoring mosquito richness in an understudied area: can environmental DNA metabarcoding be a complementary approach to adult trapping?

Published online by Cambridge University Press:  15 May 2023

Rafael Gutiérrez-López Open the ORCID record for Rafael Gutiérrez-López [Opens in a new window] ,
Bastian Egeter ,
Christophe Paupy ,
Nil Rahola ,
Boris Makanga ,
Davy Jiolle ,
Vincent Bourret ,
Martim Melo  and
Claire Loiseau
Rafael Gutiérrez-López*
Affiliation:
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBio, Laboratório Associado, University of Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal Animal Health Research Center, National Food and Agriculture Research and Technology Institute (INIA-CISA-CSIC), Valdeolmos, Spain
Bastian Egeter
Affiliation:
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBio, Laboratório Associado, University of Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal
Christophe Paupy
Affiliation:
MIVEGEC, Univ. Montpellier, CNRS, IRD, Montpellier 34394, France
Nil Rahola
Affiliation:
MIVEGEC, Univ. Montpellier, CNRS, IRD, Montpellier 34394, France
Boris Makanga
Affiliation:
Institut de Recherche en Écologie Tropicale/CENAREST, BP 13354 Libreville, Gabon
Davy Jiolle
Affiliation:
MIVEGEC, Univ. Montpellier, CNRS, IRD, Montpellier 34394, France
Vincent Bourret
Affiliation:
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBio, Laboratório Associado, University of Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal INRAE – Université de Toulouse UR 0035 CEFS, 31326 Castanet Tolosan, France
Martim Melo
Affiliation:
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBio, Laboratório Associado, University of Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal MHNC-UP – Natural History and Science Museum of the University of Porto, Porto, Portugal FitzPatrick Institute of African Ornithology, University of Cape Town, Cape Town, South Africa
Claire Loiseau
Affiliation:
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBio, Laboratório Associado, University of Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal CEFE, Université de Montpellier, CNRS, Montpellier, France
*
Corresponding author: Rafael Gutierrez Lopez, Email: rafael.gutierrez@inia.csic.es
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Abstract

Mosquito surveillance programmes are essential to assess the risks of local vector-borne disease outbreaks as well as for early detection of mosquito invasion events. Surveys are usually performed with traditional sampling tools (i.e., ovitraps and dipping method for immature stages or light or decoy traps for adults). Over the past decade, numerous studies have highlighted that environmental DNA (eDNA) sampling can enhance invertebrate species detection and provide community composition metrics. However, the usefulness of eDNA for detection of mosquito species has, to date, been largely neglected. Here, we sampled water from potential larval breeding sites along a gradient of anthropogenic perturbations, from the core of an oil palm plantation to the rainforest on São Tomé Island (Gulf of Guinea, Africa). We showed that (i) species of mosquitoes could be detected via metabarcoding mostly when larvae were visible, (ii) larvae species richness was greater using eDNA than visual identification and (iii) new mosquito species were also detected by the eDNA approach. We provide a critical discussion of the pros and cons of eDNA metabarcoding for monitoring mosquito species diversity and recommendations for future research directions that could facilitate the adoption of eDNA as a tool for assessing insect vector communities.

Keywords

Invasive speciesmetabarcodingoil palm plantationrainforestvectors

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Type
Research Paper
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Bulletin of Entomological Research , Volume 113 , Issue 4 , August 2023 , pp. 456 - 468
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Copyright © The Author(s), 2023. Published by Cambridge University Press

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