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Human papilloma viruses: a study of their prevalence in the normal larynx

Published online by Cambridge University Press:  29 June 2007

Desmond A. Nunez ,
Siân M. Astley ,
Fraser A. Lewis  and
Michael Wells
Desmond A. Nunez*
Affiliation:
Department of Otolaryngology, Leicester Royal Infirmary, Department of Clinical Medicine, University of Leeds.
Siân M. Astley
Affiliation:
Department of Otolaryngology, Academic Unit of Pathology, Department of Clinical Medicine, University of Leeds.
Fraser A. Lewis
Affiliation:
Department of Otolaryngology, Academic Unit of Pathology, Department of Clinical Medicine, University of Leeds.
Michael Wells
Affiliation:
Department of Otolaryngology, Academic Unit of Pathology, Department of Clinical Medicine, University of Leeds.
*
D. A. Nunez, F.R.C.S., ENT Department, Royal Infirmary, Leicester LE1 5WW. Fax: 0533 586082.
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Abstract

The association of human papilloma viruses (HPV) with laryngopharyngeal squamous cell carcinoma is under investigation. The suitability of control tissue in the reported series, invariably obtained from histologically normal tissue adjacent to a squamous cell carcinoma or from patients with benign laryngopharyngeal disease, is questionable. The present study determined the prevalence of HPV in a series of normal larynges.

Twelve autopsy larynges were collected. DNA was obtained by SDS proteinase K digestion. Evidence of HPV infection was documented by the polymerase chain reaction using oligonulceotide primers complementary to sequences in the E6 region of HPV types 11, 16 and 18.

Four female and eight male larynges, mean age 65 years (sd=16 years) were collected 72 hours postmortem (median value). HPV type 11 was isolated from three specimens. A 25 per cent prevalence rate for HPV 11 was found. No other HPV types were isolated.

Keywords

DNALarynxPolymerase chain reactionViruses
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 108 , Issue 4 , April 1994 , pp. 319 - 320
Copyright
Copyright © JLO (1984) Limited 1994

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Footnotes

Presented at the Otolaryngology Research Society Meeting, London, September 1992.

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