Book contents
- Frontmatter
- Contents
- Preface
- Acknowledgments
- List of abbreviations
- 1 Bringing muscles into focus; the first two millennia
- 2 Muscle metabolism after the Chemical Revolution; lactic acid takes the stage
- 3 The relationship between mechanical events, heat production and metabolism; studies between 1840 and 1930
- 4 The influence of brewing science on the study of muscle glycolysis; adenylic acid and the ammonia controversy
- 5 The discovery of phosphagen and adenosinetriphosphate; contraction without lactic acid
- 6 Adenosinetriphosphate as fuel and as phosphate-carrier
- 7 Early studies of muscle structure and theories of contraction, 1870–1939
- 8 Interaction of actomyosin and ATP
- 9 Some theories of contraction mechanism, 1939 to 1956
- 10 On myosin, actin and tropomyosin
- 11 The sliding mechanism
- 12 How does the sliding mechanism work?
- 13 Excitation, excitation-contraction coupling and relaxation
- 14 Happenings in intact muscle: the challenge of adenosinetriphosphate breakdown
- 15 Rigor and the chemical changes responsible for its onset
- 16 Respiration
- 17 Oxidative phosphorylation
- 18 The regulation of carbohydrate metabolism for energy supply to the muscle machine
- 19 A comparative study of the striated muscle of vertebrates
- 20 Enzymic and other effects of denervation, cross-innervation and repeated stimulation
- 21 Some aspects of muscle disease
- 22 Contraction in muscles of invertebrates
- 23 Vertebrate smooth muscle
- 24 Energy provision and contractile proteins in non-muscular functions
- The perspective surveyed
- References
- Author index
- Subject index
5 - The discovery of phosphagen and adenosinetriphosphate; contraction without lactic acid
Published online by Cambridge University Press: 04 August 2010
- Frontmatter
- Contents
- Preface
- Acknowledgments
- List of abbreviations
- 1 Bringing muscles into focus; the first two millennia
- 2 Muscle metabolism after the Chemical Revolution; lactic acid takes the stage
- 3 The relationship between mechanical events, heat production and metabolism; studies between 1840 and 1930
- 4 The influence of brewing science on the study of muscle glycolysis; adenylic acid and the ammonia controversy
- 5 The discovery of phosphagen and adenosinetriphosphate; contraction without lactic acid
- 6 Adenosinetriphosphate as fuel and as phosphate-carrier
- 7 Early studies of muscle structure and theories of contraction, 1870–1939
- 8 Interaction of actomyosin and ATP
- 9 Some theories of contraction mechanism, 1939 to 1956
- 10 On myosin, actin and tropomyosin
- 11 The sliding mechanism
- 12 How does the sliding mechanism work?
- 13 Excitation, excitation-contraction coupling and relaxation
- 14 Happenings in intact muscle: the challenge of adenosinetriphosphate breakdown
- 15 Rigor and the chemical changes responsible for its onset
- 16 Respiration
- 17 Oxidative phosphorylation
- 18 The regulation of carbohydrate metabolism for energy supply to the muscle machine
- 19 A comparative study of the striated muscle of vertebrates
- 20 Enzymic and other effects of denervation, cross-innervation and repeated stimulation
- 21 Some aspects of muscle disease
- 22 Contraction in muscles of invertebrates
- 23 Vertebrate smooth muscle
- 24 Energy provision and contractile proteins in non-muscular functions
- The perspective surveyed
- References
- Author index
- Subject index
Summary
THE DISCOVERY OF PHOSPHAGEN AND EARLY IDEAS OF ITS FUNCTION
In 1927 P. Eggleton & G. P. Eggleton (1), and independently Fiske & Subbarow (2), reported the existence in muscle extracts of a phosphorus compound, very labile especially in acid solution; the figures for inorganic P content of muscle found by earlier workers, using methods involving acid treatment of the extracts, were therefore open to grave doubt.
Eggleton & Eggleton, using the Briggs method (1), in which the colour due to reduced phosphomolybdate is allowed to develop during 30 min in acid solution, found that the increase in colour during this time with inorganic phosphate solutions was only some 5 %; but with extracts from resting frog's muscle the increase was several 100%. They proposed the name ‘phosphagen’ for the labile substance. The value for the true inorganic P of resting muscle, found by extrapolation back to zero time when the rate of colour development was followed, amounted to about 25 mg/100 g muscle; the phosphagen P content to about 60 mg/100 g. Estimations made in neutral or slightly alkaline solution (as in the Bell-Doisy (1) method or by precipitation with magnesia mixture) gave results approximating to the extrapolated values of the Briggs method. In rapidly induced fatigue the true inorganic phosphate increased at the expense of phosphagen P, though not all the phosphate of the disappearing phosphagen was found as inorganic P. In aerobic recovery, phosphagen quickly reappeared at the expense of inorganic P, during a time when little lactic acid removal had yet taken place.
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- Machina CarnisThe Biochemistry of Muscular Contraction in its Historical Development, pp. 77 - 97Publisher: Cambridge University PressPrint publication year: 1971