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A joint cyst nematode/late blight test for early generation screening of potato clones

Published online by Cambridge University Press:  05 March 2012

C.N.D. Lacey
Affiliation:
Plant Breeding Institute
G.J. Jellis
Affiliation:
Plant Breeding Institute
N.C. Starling
Affiliation:
Plant Breeding Institute
S.B. Currell
Affiliation:
Plant Breeding Institute
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Summary

This paper describes a technique for screening breeding material in the first clonal year for resistance to Globodera rostochiensis, G. pallida and Phytophthora infestans. Seedlings were grown in pots in an aphid-proof glasshouse and after harvest, when tubers with poor colours, extremely long stolons or very poor shape were rejected, tubers were stored at 5°C until dormancy had broken. Tubers were removed from store at weekly intervals and kept at room temperature to induce sprouting. One small tuber was planted on the surface of 100g of compost at 30 % water content in each closed transparent plastic container, as described by Phillips et al.(1980). The inoculum used was a suspension of equal numbers of eggs/larvae of both G. rostochiensis Rol and G. pallida Pa2–3 collected from beds where they had been maintained on the appropriate differential host variety. The containers were kept in a dark store room (15 to 20°C) for 6 to 7 weeks while the cysts were forming. Recording commenced when the majority of the cysts of G. rostochiensis were yellow and G. pallida were white. A few mature cysts were brown and hence not assignable to species, while some G. rostochiensis cysts were still white and wrongly attributed to G. pallida, but as only clones with dual resistance were selected this was not of practical importance.

Type
Chapter
Information
The Production of New Potato Varieties
Technological Advances
, pp. 81 - 83
Publisher: Cambridge University Press
Print publication year: 1987

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