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Maternal ageing causes changes in DNA methylation and gene expression profiles in mouse oocytes

Published online by Cambridge University Press:  08 July 2020

Guanmei Hou Open the ORCID record for Guanmei Hou [Opens in a new window]  and
Qing-Yuan Sun
Guanmei Hou
Affiliation:
College of Life Sciences, Qingdao Agricultural University, Qingdao266109, China State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing100101, China
Qing-Yuan Sun*
Affiliation:
College of Life Sciences, Qingdao Agricultural University, Qingdao266109, China State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing100101, China
*
Author for correspondence: Qing-Yuan Sun. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing100101, China. E-mail: sunqy@ioz.ac.cn
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Summary

Although it is well known that maternal ageing causes reduced oocyte quality and fertility, little information is known about its effect on germ cell epigenetics. In the present study, we compared the gene expression and DNA methylation profiles in germinal vesicle oocytes from young (8-week-old) and aged (18-month-old) mice using single-cell RNA-sequencing and single-cell whole-genome DNA methylation sequencing. We found significant differences in the data from the two groups. Oocytes from aged mice showed significant changes in the expression of some metabolism-related genes, such as mitochondria-associated genes, that was in line with our expectations. Expression of some genes associated with reproduction also showed significant differences. DNA methylation levels were also changed in oocytes from aged mice. The two groups had significant gaps in hypermethylation and hypomethylation levels on each chromosome. These data provide useful information for further understanding the mechanisms of oocyte ageing.

Keywords

Aged mouseDNA methylationGerminal vesicle prior oocytesSingle-cell sequencing
Type
Research Article
Information
Zygote , Volume 28 , Issue 5 , October 2020 , pp. 360 - 366
Copyright
© Cambridge University Press 2020

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