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Chapter 8 - Current Status of Immunohistochemistry

Published online by Cambridge University Press:  16 June 2022

By
Trung Nguyen
Edited by
Trung Nguyen
Trung Nguyen
Affiliation:
Peter MacCallum Cancer Centre, Australia
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Summary

The current utilization of immunohistochemistry (IHC) in a diagnostic context is discussed. The modern facility requirements, the various roles IHC is tasked with and the key concept of standardization are covered. Common terminologies are addressed and explained within an IHC context. The terms 'validation' and 'verification' provide one example of words which may cause confusion. The present status in terms of protocol set-up, antibody clones and epitope retrieval are offered to emphasize current best practice. A treatise is given concerning IHC’s special relationship with emerging molecular technologies and how these two analytical devices are shaping diagnoses and treatment strategies for patients. Specific examples are taken from melanoma, breast, lung and bowel cancers. The reader should be able to ascertain the role of IHC in today’s pathology laboratories.

Keywords

personalized medicinemolecular techniquesin situ hybridizationcompanion diagnosticscheckpoint inhibitorsmutationbiomarkerstandardizationhistopathology
Type
Chapter
Information
Immunohistochemistry
A Technical Guide to Current Practices
 , pp. 210 - 252
Publisher: Cambridge University Press
Print publication year: 2022

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