Book contents
- Frontmatter
- Contents
- Preface to the second English edition
- Preface to the first edition
- Introduction
- 1 Abbe's sine condition
- 2 Fourier optics
- 3 Effect of polarization on diffraction in systems of high numerical aperture
- 4 Gaussian beam optics
- 5 Coherent and incoherent imaging
- 6 First-order temporal coherence in classical optics
- 7 The van Cittert–Zernike theorem
- 8 Partial polarization, Stokes parameters, and the Poincaré sphere
- 9 Second-order coherence and the Hanbury Brown–Twiss experiment
- 10 What in the world are surface plasmons?
- 11 Surface plasmon polaritons on metallic surfaces
- 12 The Faraday effect
- 13 The magneto-optical Kerr effect
- 14 The Sagnac interferometer
- 15 Fabry–Pérot etalons in polarized light
- 16 The Ewald–Oseen extinction theorem
- 17 Reciprocity in classical linear optics
- 18 Optical pulse compression
- 19 The uncertainty principle in classical optics
- 20 Omni-directional dielectric mirrors
- 21 Linear optical vortices
- 22 Geometric-optical rays, Poynting's vector, and the field momenta
- 23 Doppler shift, stellar aberration, and convection of light by moving media
- 24 Diffraction gratings
- 25 Diffractive optical elements
- 26 The Talbot effect
- 27 Some quirks of total internal reflection
- 28 Evanescent coupling
- 29 Internal and external conical refraction
- 30 Transmission of light through small elliptical apertures
- 31 The method of Fox and Li
- 32 The beam propagation method
- 33 Launching light into a fiber
- 34 The optics of semiconductor diode lasers
- 35 Michelson's stellar interferometer
- 36 Bracewell's interferometric telescope
- 37 Scanning optical microscopy
- 38 Zernike's method of phase contrast
- 39 Polarization microscopy
- 40 Nomarski's differential interference contrast microscope
- 41 The van Leeuwenhoek microscope
- 42 Projection photolithography
- 43 Interaction of light with subwavelength structures
- 44 The Ronchi test
- 45 The Shack–Hartmann wavefront sensor
- 46 Ellipsometry
- 47 Holography and holographic interferometry
- 48 Self-focusing in nonlinear optical media
- 49 Spatial optical solitons
- 50 Laser heating of multilayer stacks
- Index
- References
38 - Zernike's method of phase contrast
Published online by Cambridge University Press: 31 January 2011
- Frontmatter
- Contents
- Preface to the second English edition
- Preface to the first edition
- Introduction
- 1 Abbe's sine condition
- 2 Fourier optics
- 3 Effect of polarization on diffraction in systems of high numerical aperture
- 4 Gaussian beam optics
- 5 Coherent and incoherent imaging
- 6 First-order temporal coherence in classical optics
- 7 The van Cittert–Zernike theorem
- 8 Partial polarization, Stokes parameters, and the Poincaré sphere
- 9 Second-order coherence and the Hanbury Brown–Twiss experiment
- 10 What in the world are surface plasmons?
- 11 Surface plasmon polaritons on metallic surfaces
- 12 The Faraday effect
- 13 The magneto-optical Kerr effect
- 14 The Sagnac interferometer
- 15 Fabry–Pérot etalons in polarized light
- 16 The Ewald–Oseen extinction theorem
- 17 Reciprocity in classical linear optics
- 18 Optical pulse compression
- 19 The uncertainty principle in classical optics
- 20 Omni-directional dielectric mirrors
- 21 Linear optical vortices
- 22 Geometric-optical rays, Poynting's vector, and the field momenta
- 23 Doppler shift, stellar aberration, and convection of light by moving media
- 24 Diffraction gratings
- 25 Diffractive optical elements
- 26 The Talbot effect
- 27 Some quirks of total internal reflection
- 28 Evanescent coupling
- 29 Internal and external conical refraction
- 30 Transmission of light through small elliptical apertures
- 31 The method of Fox and Li
- 32 The beam propagation method
- 33 Launching light into a fiber
- 34 The optics of semiconductor diode lasers
- 35 Michelson's stellar interferometer
- 36 Bracewell's interferometric telescope
- 37 Scanning optical microscopy
- 38 Zernike's method of phase contrast
- 39 Polarization microscopy
- 40 Nomarski's differential interference contrast microscope
- 41 The van Leeuwenhoek microscope
- 42 Projection photolithography
- 43 Interaction of light with subwavelength structures
- 44 The Ronchi test
- 45 The Shack–Hartmann wavefront sensor
- 46 Ellipsometry
- 47 Holography and holographic interferometry
- 48 Self-focusing in nonlinear optical media
- 49 Spatial optical solitons
- 50 Laser heating of multilayer stacks
- Index
- References
Summary
Zernike invented the phase-contrast microscope in 1935, and was awarded the 1953 Nobel prize in physics for this achievement. In an ordinary optical microscope, an object that imparts a phase modulation to the incident light will produce only a faint image. This faint image may be attributed to the diffraction of a small amount of the light out of the entrance pupil of the objective lens. To improve this image, Zernike in effect extracted a reference beam from the light collected by the objective lens and produced an interferogram of the object at the image plane of the microscope, thus converting phase information into amplitude (or intensity) modulation.
The principles of operation of the phase-contrast microscope are by now fully understood. Both spatially coherent and spatially incoherent light may be used in this type of microscopy. For best results, a quasi-monochromatic light source with a reasonable coherence time must be employed. Our goal in the present chapter is to give a simple explanation of the main ideas behind the method and to provide a pictorial survey of this important branch of modern optical microscopy.
The phase-contrast microscope
The diagram in Figure 38.1 shows the main elements of a phase-contrast microscope. The light source may be a coherent source (e.g., a laser) or an incoherent one (e.g., a tungsten lamp or an arc lamp); monochromaticity may be achieved by means of a colored glass filter. The condenser lens projects the source onto the object, whose image is formed by the objective lens.
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- Information
- Classical Optics and its Applications , pp. 545 - 553Publisher: Cambridge University PressPrint publication year: 2009